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We narrowed to 1,074 results for: CAN

Showing: 101 - 120 of 1074 results

  1. Adenovirus Guide

    Type
    Guide
    ... Adenoviridae family and can infect dividing and non-dividing cells. AdVs can cause a range of mild diseases...system recognizes adenoviral elements rapidly and can mount a strong immune response quickly, which has... of interest. The size of the genetic cargo that can be included between the ITRs is limited by the physical..., or shRNA of interest into the transfer plasmid can be done with most standard cloning methods, including...transgene into the adenoviral backbone plasmid. One can also co-transform standard BJ5183 with supercoiled... with the linearized construct, and rAdV vectors can be harvested about 7–10 days later. Vogelstein designed... transgene of interest. With these plasmids, one can track the infection of HEK293/HER911 cells throughout...

  2. Adeno-associated virus (AAV) Guide

    Type
    Guide
    ...genome can have on transgene expression and the expression of neighboring genes. rAAV episomes can develop... plasmids can be used to create viral vectors capable of infecting target cells, but cannot produce any...genus, included within the Parvoviridae family. They can infect dividing and non-dividing cells; however, ... of interest. The size of the genetic cargo that can be included between the ITRs is limited by the physical..., or shRNA of interest into the transfer plasmid can be done with most standard cloning methods, including...integration is mediated by proteins Rep78 and Rep68, which can bind to specific Rep-binding elements present in ...processed into a double-stranded circular episome which can concatemerize, producing high molecular weight structures...

  3. Guide to Using Pooled Libraries

    Type
    Guide
    ... use a pooled library; and what types of screens can be done with a pooled library....plasmids with unique, semi-random sequences that can be used for applications like lineage tracing or ...expressing multiple genes at once. A well-designed screen can help you begin to understand what genes are important...population of plasmids in a single tube. Pooled libraries can be small, if they are designed to cover only a subset...the best results. For some libraries, plasmid DNA can be delivered directly to the cells of interest. With...positives or false negatives in later experiments, and can also negatively affect data reproducibility. Types...biological replicate for that plasmid. Library screens can be divided into two types: positive screens and negative...

  5. Chemogenetics Guide

    Type
    Guide
    ...channels (LGICs) that can either excite or inhibit neuronal signaling. PSAM-based LGICs can be classified by...specific cell populations (most often neurons) that can be turned on or off by the application of a small...neurotransmission. This, and the fact that GPCR ligands can have high potency, which is potentially important...There are several different types of DREADDs that can be broadly classified by the signaling protein that...clozapine and other clozapine metabolites and these can have off-target effects. More recent studies have.... Scientists have shown that both KORD and hM3Dq can be used in the same organism to allow bidirectional...in our Optogenetics Guide . Figure 4: Luminopsins can be activated through chemogenetic or optogenetic ...

  6. Synthetic Biology - Overview

    Type
    Collection
    ...facilitate the sharing of parts and information. Addgene can help you to share your synthetic biology plasmids...

  7. Promoters

    Type
    Guide
    ...activator can bind, and the structural genes that are transcribed together. Operon regulation can be either... stably and transcription of the template strand can initiate. The TATA box is a DNA sequence (5'-TATAAA...general transcription factor proteins and histones can bind. Histones are proteins found in eukaryotic cells...explain how many physically distant DNA sequences can affect transcription of a given gene. The TATA-binding...in the absense of lac repressor (lacI or lacIq). Can be induced by IPTG or lactose Promoter from Lac operon...

  8. Recombinases AAV Preps

    Type
    Collection
    ...preps that encode site-specific recombinases, which can be used to control gene expression. See our Cre-lox...

  9. Retrograde AAV viral preps

    Type
    Collection
    ... neurons. Specific classes of projection neurons can be targeted by using this serotype to deliver Cre-dependent...lab. Results for our quality control experiments can be found on the material pages for viral catalog ...

  10. Optogenetics Guide

    Type
    Guide
    ... and the neuron is activated. Optogenetics tools can be broadly classified based on their functions into...Microbial opsins, such as those described below, can be targeted and expressed in specific subsets of ... neuronal activation/inactivation periods. These can range from msec (hChR2) to “long lasting,” e.g. seconds... in the figure above, this light induced binding can be utilized to control the localization of a protein...interest. A genetically-encoded nuclear Cry2 fusion can thus direct a CIB1-protein fusion to the nucleus ...half to CIB1, light-stimulated heterodimerization can reconstitute the protein. This principle has also...applications described above, both LOV and Dronpa can control activity of a fused protein through allosteric...

  12. CRISPR Plasmids - Prime Edit

    Type
    Collection
    ... mutations in Cas9 to enhance nuclease activity; can be used in PE2–PE5 systems PE6 - small prime editors...

  13. AAVED

    Type
    Collection
    ... a general overview of the technology and how it can be used for targeted neuronal manipulation. Topics...

  14. CRISPR Pooled gRNA Libraries

    Type
    Collection
    ...organism. All of our current pooled CRISPR libraries can be found in the following table, which also includes...species. Additional information about each library can be found on the individual library page. Find...Library 138015 Knockout Mouse Pu N/A 6 14,671 Pan-cancer CasRx lncRNA Albarossa Library 212966 Knockout ... D. melanogaster Liu N/A 3 40,279 Pan-Druggable Cancer Library 182133 Knockout Human Mali 3rd 1 74 Perturb-seq...

  15. p53 Pathway

    Type
    Collection
    ... at one of six hotspot residues. These mutations can be inherited or arise through exposure to mutagens... Cancer Research p53 Pathway p53 Pathway You May Also Like: Cancer Pathway ORF Kit mTOR...SDS-PAGE gel. Cancer Impact p53 is the most frequently mutated gene (>50%) in human cancer; most of these...mechanisms by which mutant p53 is known to function in cancer. p53 Pathway Plasmids Click on a name to find available...component 3 Reprimo TP53 dependent G2 arrest mediator candidate Scotin Shisa family member 5 Sestrins SESN1 SESN2...suppression. Bieging KT, Mello SS, Attardi LD. Nat Rev Cancer. 2014 May;14(5):359-70. PubMed PMID: 24739573 ....the mutant p53 field. Brosh R, Rotter V. Nat Rev Cancer. 2009 Oct;9(10):701-13. PubMed PMID: 19693097 ....

  17. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ...biosensors can be introduced into cells, tissues, or organisms for long-term imaging and can be designed...conditions. With proper experimental design, biosensors can enable investigation of a signaling pathway or measurement...an existing construct, as fluorescence properties can be affected by environmental factors and the sensor...1147-1155. Lin Tian Endocannabinoid GRAB_eCB2.0/eCBmut biosensor for endocannabinoids in vivo (Constitutive...peroxide Monitoring the action of redox-directed cancer therapeutics using a human peroxiredoxin-2-based...sensor for spatiotemporally resolved imaging of endocannabinoid dynamics in vivo. Nat Biotechnol. 2021 Nov ...

  18. Control AAV Preps

    Type
    Collection
    ...fluorescent proteins under general promoters. These AAV can be used to compare the activity of different serotypes...

  19. Science Guides

    Type
    Guide
    ...use antibodies conjugated to a signaling molecule, can be used to capture, quantify, and identify proteins...
Showing: 101 - 120 of 1074 results