...process and have your plasmids added to the list. You can also email [email protected] for more information ...

...single or multicolor imaging. Photo-activatable iRFPs can be ‘turned on’ by non-phototoxic far-red light and...

...FLASH, including detailed and up-to-date protocols, can be found on the TALengineering website. Users may...

... from Addgene, and by providing this service, we can save you time and help you get results faster. Choose...! Browse AAV | Browse Lenti Packaged on Request Can't find what you need in our in-stock preps? Our Packaged...

... use a pooled library; and what types of screens can be done with a pooled library....plasmids with unique, semi-random sequences that can be used for applications like lineage tracing or ...expressing multiple genes at once. A well-designed screen can help you begin to understand what genes are important...population of plasmids in a single tube. Pooled libraries can be small, if they are designed to cover only a subset...the best results. For some libraries, plasmid DNA can be delivered directly to the cells of interest. With...positives or false negatives in later experiments, and can also negatively affect data reproducibility. Types...biological replicate for that plasmid. Library screens can be divided into two types: positive screens and negative...

... . If you have further questions or comments you can also contact them at [email protected] ....

...provides the widest tropism or range of cells a virus can infect. Overview of Retroviral Plasmid System Note...modify tropism. Briefly, different envelope plasmids can direct the production of virus with various tropisms...HIV for lentivirus). Additionally, γ-retroviruses can only transduce dividing cells because they are only... breakdown of the nuclear envelope. Lentiviruses can transduce both dividing and non-dividing cells because...plasmids or the packaging cell line. γ-retroviruses can transduce target cells, but the transduced target...Are γ-retroviruses self-inactivating (SIN)? They can be. Plasmids that encode SIN γ-retrovirus lack most...widespread use of γ-retroviruses (gamma-retroviruses) in cancer and stem cell research has prompted the development...

...channels (LGICs) that can either excite or inhibit neuronal signaling. PSAM-based LGICs can be classified by...specific cell populations (most often neurons) that can be turned on or off by the application of a small...neurotransmission. This, and the fact that GPCR ligands can have high potency, which is potentially important...There are several different types of DREADDs that can be broadly classified by the signaling protein that...clozapine and other clozapine metabolites and these can have off-target effects. More recent studies have.... Scientists have shown that both KORD and hM3Dq can be used in the same organism to allow bidirectional...in our Optogenetics Guide . Figure 4: Luminopsins can be activated through chemogenetic or optogenetic ...

...activator can bind, and the structural genes that are transcribed together. Operon regulation can be either... stably and transcription of the template strand can initiate. The TATA box is a DNA sequence (5'-TATAAA...general transcription factor proteins and histones can bind. Histones are proteins found in eukaryotic cells...explain how many physically distant DNA sequences can affect transcription of a given gene. The TATA-binding...in the absense of lac repressor (lacI or lacIq). Can be induced by IPTG or lactose Promoter from Lac operon...

...bacterial stabs at room temperature. Individual plasmids can be ordered from each plasmid page and will be shipped...

...chromatin structure at the site of genome integration can affect the expression of transgenes, as well as the...through double stranded synthesis. These episomes can concatemerize, producing high molecular weight structures...particles produced during a lytic cycle. rAAV episomes can develop chromatin-like organization and persist in...from serotype 5. Use of these pseudotyped viruses can improve transduction efficiency, as well as alter... spliced together, and the full-length transgene can then be expressed. This method allows for longer ...efficiency (<1% of wild type). If either of these methods can be made more efficient, the use of AAV viruses would...frequency. As a replication-incompetent virus, AAV cannot enter the lytic cycle without help. Another virus...

...between institutions. With these plasmids, faculty can begin a sustainable MDH-related CURE focusing on ...

...facilitate the sharing of parts and information. Addgene can help you to share your synthetic biology plasmids...

... and the neuron is activated. Optogenetics tools can be broadly classified based on their functions into...Microbial opsins, such as those described below, can be targeted and expressed in specific subsets of ... neuronal activation/inactivation periods. These can range from msec (hChR2) to “long lasting,” e.g. seconds... in the figure above, this light induced binding can be utilized to control the localization of a protein...interest. A genetically-encoded nuclear Cry2 fusion can thus direct a CIB1-protein fusion to the nucleus ...half to CIB1, light-stimulated heterodimerization can reconstitute the protein. This principle has also...applications described above, both LOV and Dronpa can control activity of a fused protein through allosteric...

...preps that encode site-specific recombinases, which can be used to control gene expression. See our Cre-lox...

... neurons. Specific classes of projection neurons can be targeted by using this serotype to deliver Cre-dependent...lab. Results for our quality control experiments can be found on the material pages for viral catalog ...

...with dual labeling when used with two substrates Can be used in live or fixed cells, and for internal ...

... mutations in Cas9 to enhance nuclease activity; can be used in PE2–PE5 systems PE6 - small prime editors...

... a general overview of the technology and how it can be used for targeted neuronal manipulation. Topics...

...organism. All of our current pooled CRISPR libraries can be found in the following table, which also includes...species. Additional information about each library can be found on the individual library page. Find...Library 138015 Knockout Mouse Pu N/A 6 14,671 Pan-cancer CasRx lncRNA Albarossa Library 212966 Knockout ... D. melanogaster Liu N/A 3 40,279 Pan-Druggable Cancer Library 182133 Knockout Human Mali 3rd 1 74 Perturb-seq...