...Michael Davidson’s lab at Florida State University has been the go-to place. In 2012, his National High ...backbones alone,” said Addgene’s Lianna Swanson, who has been working with members of Davidson's lab to coordinate...coordinate the impressively big deposit. “He has every fluorescent protein under the sun, from the standard... easy proposition, explained Michelle Baird, who has created plasmids as a technician in the Davidson ...Connection.  mEmerald plasmids and many more! Baird has a helpful tip for anyone looking to peruse the collection...from the nucleus to cell membrane, this collection has fluorescently tagged versions of many proteins known...his life’s work and the stage IV lung cancer that has now prompted him to deposit the tools he and his ...

...page. You’ve called. You’ve emailed. And Addgene has been there to answer your plasmid questions. But ...Addgene’s request statistics Since 2004, Addgene has shipped more than 75,000 plasmids to more than 1,...alone, the number of plasmids distributed to Europe has doubled going from 9,500 in 2010 to more than 20,...London, Emma Markham. Emma was born in England but has since studied and worked around the world, carrying...California, specialising in Molecular Biology. She has worked in a range of labs - cancer research, microbiology...Imperial College London in Genetics. Since then she has worked in the the UK, California, Africa, and Australia... exchange research materials in an easy way. She has a passion for science, loving nothing more than discussing...

...antibiotic required for effective over-agar selection has been empirically determined. See selection curve ...

...As we mentioned a few months ago, Addgene has been working with Seeding Labs to provide plasmid grants...their research. Louis Bengyella Dr. Bengyella’s work has focused on elucidating the molecular mechanisms behind...potatoes and their microbial pathogens. This work has given Dr. Bengwella expertise in using bioinformatic...Kwabena O. Duedu Dr. Duedu’s molecular biology work has focused on using various cloning methods and tools...Future awardees Now that this first round of awards has successfully concluded, Addgene and Seeding Labs ...

...This competition has gained so much public interest for its stunning images that is has been featured in...Over the past decade there has been a shift in how science is perceived - where once science was only...Queensland, whose outstanding marine photography has attracted much attention over the past few years,... went on to discuss his work: “Macro photography has been my passion for a while - a tool to expand perception...non-scientist alike. “The other thing photography has shown to me - it requires an awful amount of skill...

...Professor of Chemistry at Connecticut College who has been teaching and communicating science for over ... steadily increasing distrust the general public has in science and scientists (as discussed in Chapter...biology also falls within this section. The GFP story has its own Franklin in Doug Prasher, who originally ...advances, and posits the way science is being done has changed significantly over the last decade. He describes... distribution of the newest CRISPR-Cas plasmids, has been a key driver of the technology.” As an Addgene..., I’m very familiar with the role our repository has played in these fields, and seeing names like Feng...have not changed in “COVID times,” but the pandemic has certainly exacerbated them and forced scientists ...

...Repair has been viewed 219,289 times, while Validating Your Genome Edit has 76,011 views.  Has all of ...libraries Addgene has distributed (1C), from 2012 to 2023. Addgene’s collection of CRISPR tools has grown steadily...2023, our CRISPR guide has been viewed 1,904,462 times, and the CRISPR 101 eBook has been downloaded over...globe. Our first story focuses on a technology that has changed virtually all of biology…CRISPR.  Since ...own to share. Over the years, Addgene’s repository has grown to include many CRISPR tools.  Distributing...

...enzymes on the block? The Cas family of proteins has many useful genome engineering enzymes, each with...to generate a blunt DNA double strand break. Cas9 has been studied for genome engineering purposes far ...far more than any other Cas family protein and has thus benefitted from countless rounds of optimization...effective at wiping out large regions of DNA and has anti-viral application potential as a result. Fast... Cas enzymes outlined below as well. Cas12a also has significant therapeutic promise due to the fact that...to package for therapeutic delivery by AAV, which has strict capacity limitations. New efforts have recently...for efficient, multiplexable RNA knockdown. Cas13 has also stirred up interest for its potential as a viral...

...some of the great biological societies that Addgene has had the opportunity to work with at Freshers Fairs...Society, England Since 1996, Cambridge Biosoc’s goal has been to promote interest in the biological sciences...students with their applications. The society currently has 1,283 members, who comprise undergraduates, graduate...who want to study chemistry. The society currently has 352 members, ranging from undergrad all the way up...Society based at the University of Oxford currently has around 700 members and hosts a range of scientific...College London for students in this emerging field. It has gained over 170 members in its first year. The society...

...immunoglobulin intron that has been commonly used in constitutive splicing studies and has been optimized for ...light-activated protein domains to the appropriate locus has been cumbersome. To overcome many of the limitations...effectors or Zinc Finger Nucleases, the Gersbach lab has modified the RNA-guided CRISPR-Cas9 system to create...co-localize the VP64 transactivator with a dCas9 that has been targeted to a very specific site on the genome...intracellular pH regulation is important as pH regulation has roles in many cellular functions, from endosomal ..., this peptide is caged by the asLOV2 domain and has reduced affinity for its binding target, SspB. In...activating endogenous genes Moritoshi Sato's Lab has engineered CRISPR-Cas9 to create a photoactivatable...

...protein–protein interactions. The Roux lab has deposited HA tagged BioID2 for N-terminal fusions and Myc... activation of these receptors. Bryan Roth's lab has a newly available PRESTO-TANGO kit which allows researchers...the NCI RAS Initiative, led by Dominic Esposito, has recently released a collection of 360 Ras pathway...24395366 Oxford Drosophila CRISPR library Addgene has had the privilege of distributing several human and...this 3-day goal is is now attainable. The Jacks lab has recently introduced a new assembly cloning platform...Addgene to help expand the collection! The Jacks lab has made several GMAP-compatible plasmids available to...laboratory of Jason Moffat at the University of Toronto has created the Toronto KnockOut (TKO) CRISPR Library...

...Eric Perkins to discuss the various positions he has held in his career, his favorite company tradition...

...wildtype Cas9 with optimized chimeric gRNA has high efficiency but has been shown to have off-target effects... the Genome Engineering Using CRISPR/Cas Systems has expanded out of control. To further consolidate these... a new system, developed by the Zhang lab, which has comparable efficiency to the optimized chimeric design...recombination? A3: The most we've tried to insert so far has been 1kb. We used homology arms that were 800bp long...for positive ones.  -- OR -- If your HR template has a selection marker such as puromycin, you can (also...

... requested ubiquitin plasmids were pRK5-HA-Ubiquitin-WT and HA-Ubiquitin, and we now have 19 different...founded 10 years ago today. In that time, Addgene has shipped over 350,000 individual plasmids to 5,000...5,000 different research institutions. This has given us a unique window into technology trends in the life...the CRISPR." The newest genome editing technology has been used and modified by so many labs and for so...

...and divide along with the endogenous DNA. AAV also has low immunogenicity, meaning it does not induce the...conducive to targeting all cell types. Altogether, AAV has many excellent features for human gene therapy and...administration, such as in mouse experiments. AAV also has multiple serotypes (12 naturally occurring and more...constraints are an issue. Adenovirus Adenovirus has a linear, double stranded DNA genome which is non-enveloped...cancer in hamsters, although no cancer-connection has been identified in humans to date. Over fifty serotypes...have been identified so far! Adenovirus generally has high immunogenicity, but some strains have been engineered...other viruses discussed in this article, adenovirus has the highest maximum titer, a plus for production ...

...Resource Center has various plasmids for overexpression and knockdown. Addgene also has a number of insect...locus your transgene ends up (1): Random insertion has the advantage that you can get varying expression... to determine in which chromosome your transgene has integrated. This can be accomplished with a few matings... embryos and pick out flies where your transgene has been incorporated into the germline. Outsourcing ... have to map into which chromosome the transgene has integrated and make genetic crosses with balancer...finishing his immunology PhD at Harvard University. He has had nearly a decade of experience working with Drosophila...

...lab at the Massachusetts Institute of Technology has been “working on new experimental and theoretical...and medicine. As they’ve gone along, Voigt’s group has also been assembling the toolbox needed for anyone...CRISPR circuitry, it can slow their growth. Something has to be done about that.  The second problem is fundamental...this now, we have ideas, but so far nothing that has worked. I don’t have the solution. Our paper shows...transcriptional and translational activity a cell has and control how much it taxes the resources of a ...antibodies. But we are really just using what biology has given us. Wood is a complicated material and we can...can access it, but we are stuck with what biology has given us. Over the next 100 years, we are really ...

...CRISPR has greatly enhanced the ability of scientists to make genomic alterations, bringing about a revolution...inserting a custom gRNA.  While this methodology has proven to be incredibly valuable to scientists, there... used to deliver a Cas9-gRNA RNP. This advantage has allowed scientists to perform in vitro and in vivo...to the use of this technique in cell culture, it has also been applied to genome editing of mouse zygotes...the early stages of development. David Liu’s Lab has demonstrated the use of cationic lipid-mediated methods...et al. 2015). A more targeted approach using RNPs has recently been described in vitro, with the hope of..., as an alternative to plasmid based techniques, has been an area of intense study. Two methods to accomplish...

...CRISPR/Cas9 has made it easier than ever to make precise, targeted genome modifications. Cas9 has been modified...which one of Cas9’s two critical enzymatic residues has been converted to an alanine (D10A or H840A), changing... for your experiment, ensure your sgRNA sequence has little homology to other regions of the genome and...looser PAM requirements compared to SpCas9. xCas9 has a PAM requirement of NG, GAA, or GAT, making it a...SpG variant, discovered by the Kleinstiver group has a PAM of NGN (Walton et al., 2020). Further, this...reverse transcriptase, and a nCas9. While this system has been shown to be less efficient than base editing...directly fusing the cargo to dCas9.  This technique has been exploited to localize factors such as transcriptional...

...give me a brief history of optogenetics? How long has this idea been around? Boyden: Optogenetics is a ... silencers of neural activity. Since then, there has been a steady stream of papers describing new photosynthetic...against each other. Getting two-color stimulation has been a holy grail of the field. The problem is that...is fundamental understanding of the brain, which has humanistic and philosophical implications. As an ...Addgene over the years, many of them very popular. Why has it been so important to you to distribute these tools...as possible? Boyden: I think because neuroscience has so many different goals: to understand how a mouse...