...Stable RNA <8 Kb 80-130 Dividing Yes Moderate * Recombinant AAV has a low frequency of target cell genome... Lab Lentivector Toolbox Guide to Generating Recombinant Adenoviruses Eric Campeau's Viral Vector Guide...Toth K. Adenovirus vectors for gene therapy, vaccination and cancer gene therapy. Curr Gene Ther. 2013...

...9N7polA (CT) 9 degrees N-7 DNA Polymerase chain terminating 165511 pOpen-K12polLF DNA Polymerase I, Large...methyltransferase 165520 pOpen-T4 PNK T4 PNK polynucleotide kinase 165521 pOpen-lambda red operon lambda red operon...pOpen-BovDNTT Bovine DNTT 165555 pOpen-ALPI CIP (calf intestinal phosphatase) Return to top Additional Resources...

... found on this page: SARS-CoV-2 Antibodies : Recombinant monoclonal antibodies that target SARS-CoV-2 ... Nucleocapsid Protein [mBG17] - Clone mBG17, recombinant mouse monoclonal antibody targeting the SARS-... Nucleocapsid Protein [mBG86] - Clone mBG86, recombinant mouse monoclonal antibody targeting the SARS-...

...guanosine. RNA editors : Cas13 fused to the ADAR2 deaminase convert adenosine to inosine on RNA. Inosine is..., Davidson AR. 2013. Bacteriophage genes that inactivate the CRISPR/Cas bacterial immune system. Nature...Cell . 154(2):442-51. PMID: 23849981 Ishino Y, Shinagawa H, Makino K, Amemura M, Nakata A. 1987. Nucleotide...

...Overview of Addgene's ready-to-use monoclonal recombinant antibody collection. These affinity reagents ... Antibodies Addgene distributes ready-to-use recombinant antibodies to help facilitate scientific research...

...assembly are different among the three methods, the final products are the same). All expression vectors listed...Sander et al., Nat Biotechnol. 2011 ): ID Plasmid C-terminal 0.5 TALE repeat Binds 32285 JDS70 NI RVD A 32287...

...Catalytically inactive CRISPR plasmids for purification of target loci.... molecule-mediated ChIP) system, catalytically inactive dCas9 is used to purify genomic DNA bound by the...

...Expression vector for secreted proteins with N-terminal hexahistidine tag and TEV cleavage, Bac-to-Bac...Expression vector for secreted proteins with C-terminal hexahistidine tag and TEV cleavage, Bac-to-Bac...

...a ntiviral de fense) complex, comprised of a combination of Cas5, Cas6, Cas7, Cas8, and Cas11. Cascade...non-specifically along the way. Cas3 initiates a final double-strand break, either due to a boundary defined...

...partnership of 13 members from industry and academia financed by the Innovative Medicine Initiative (IMI) of...Figure 1: Representation of RESOLUTE mission. The combination of metabolomics and genome engineering forms ...

...Information Protocols & Resources Contents of Kit Original Publication Oligomerized pool engineering (OPEN...tools for using the bacterial two-hybrid system originally described by Hochschild and colleagues ( Dove...

...institutions. With these plasmids, faculty can begin a sustainable MDH-related CURE focusing on protein expression...experiences (CURE) impacts student learning and attitudinal outcomes: A study of the Malate dehydrogenase...

...the most common of which involves homologous recombination of adenovirus plasmids in either mammalian cells...Strain Description PI 16398 BJ5183 Strain for recombination between shuttle plasmid and adenoviral plasmid...

... Catalytically dead dCas9 fused to a cytidine deaminase protein becomes a specific cytosine base editor...Selectable Marker PI Publication Purify A catalytically inactive Cas9 (dCas9) can be used to purify a region of...

...and Dutzler Labs EasyClone 2.0 Yeast Toolkit - Borodina Lab Golden GATEway cloning system - Wittbrodt ...MoClo : Modular cloning for applications like recombinant protein purification and cell-free circuit design...

.... elegans by combining expression of the FLP recombinase from either a heat-shock promoter or a tissue-specific...WormBook - A comprehensive, open-access collection of original, peer-reviewed chapters covering topics related...

...carryover from the HV in final viral preparations. Several methods to avoid HV contamination have been developed...in a higher background of non-recombinant adenoviral plasmids. Recombinant adenoviral plasmids are then... other patterns of recombination have not occurred. Once verified, the recombinant plasmid is linearized...vectors for recombinant adenoviral vector production that do not contain GFP. Recombinant adenoviral vector...heavily-spliced transcripts flanked by two inverted terminal repeats (ITRs). Adenoviral genes are divided into...Parvoviridae family. AAVs were discovered as a contaminant of adenovirus preparations, which is how they...information about AAVs, read our AAV guide . Recombinant Adenoviral Vectors Wild-type adenoviruses have...

... Catalytically dead dCas9 fused to a cytidine deaminase protein becomes a specific cytosine base editor...Promoter PI Publication Purify A catalytically inactive Cas9 (dCas9) can be used to purify a region of...

...TALEN Kits CRISPR Plasmids Zinc Finger Consortium Originally published in Addgene's December 2011 Newsletter...Bogdanove Golden Gate TALEN kit. In this example, the final array is composed of 18 TAL effector repeats. The...

... catalyze cytidine deamination instead of (or in addition to) adenosine deamination, producing cytosine...Plasmids: CRISPR transposases CRISPR Recombinases and Integrases Recombinases, and their subfamily integrases...interesting adaptive immune response that detects and eliminates foreign DNA, which scientists have modified for...target sequence present in the gRNA. CRISPR was originally employed to knock out target genes in various...SpCas9, has one active nuclease domain and one inactivated one, allowing it to generate DNA nicks by cutting...cutting only one strand. Catalytically inactive “dead” Cas9 (dCas9) contains mutations in both nuclease...backbone HypaCas9 - increase Cas9 proofreading and discrimination evoCas9 - decrease off-target effects xCas9...