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...102. Joanna Bereta Sybody Generation Toolbox for making synthetic nanobodies (sybodies) against membrane...

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...experiments. Shipping 2–7 days We will email you with tracking information as soon as your order ships. Shipping...

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... the archaeal origins of Eukaryotic Membrane-Trafficking System building blocks. Molecular Biology and...

...Browse Plasmids Plasmid Tools for Use in Zebrafish Looking for plasmids containing your zebrafish gene of ...

...1464-1478. PMID: 32304666 Don’t See What You’re Looking For? Our Packaged on Request service offers you...

...information to help plan your next FRET experiment. Looking for FRET-based biosensors? Check out our Biosensors...

... You can search the table for features you are looking for, such as the promoter or cloning method of ...

...Retrograde AAV Inventory Page . Don’t See What You’re Looking For? Our Packaged on Request service offers you...

...protocol pU6-BbsI-chiRNA ; phsp70-Cas9 PDF, 109 KB Orkin and Bauer Protocol for Genomic Deletions in Mammalian...

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...of California, Berkeley). Don’t See What You’re Looking For? Our Packaged on Request service offers you...

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...mutations to alter the absorption spectrum or adding trafficking signals to localize opsins to the cell membrane...research, helping scientists to better understand Parkinson's disease, depression, drug addiction, and more...), C1V1(t/t), ChIEF Increased channel (on/off) kinetics: Examples: ChETA, C1V1(t/t), ChrimsonR Red-shifted...CrChR2 450 ChETA E123T mutation; creates faster kinetics but reduces photocurrent amplitude 490 ChR/T159C...activity 589 eNpHR 3.0 NpHR with addition of trafficking signal from Kir2.1 and ER export signal provide...sodomense 566 eArch 3.0 Arch with addition of trafficking signal from Kir2.1 566 ArchT Light-activated ... Arch. 566 eArchT 3.0 ArchT with addition of trafficking signal from Kir2.1 566 Leptosphaeria rhodopsins...

...Molecular Genetics The Genetic Code Recombinant DNA Working with Plasmids Plasmid Elements Types of Plasmids... genes. Figure 1: Creation of recombinant DNA. Working with Plasmids Today, scientists can easily study...to break chromosomes down to study their genes, marking the birth of molecular genetics. To learn more ... number of E. coli strains below and recommend checking out Addgene’s blog posts about common E. coli ...and their recommended concentrations. We suggest checking your plasmid's datasheet or the plasmid map to...Antibiotic Recommended Stock Concentration Recommended Working Concentration Ampicillin 100 mg/mL 100 µg/mL Carbenicillin... to the sequence of the complementary strand. Mimicking the DNA replication process requires the four ...

...dynamic tracking of repetitive and non-repetitive genomic loci, chromosome painting, and RNA tracking in living... the most scalable genome editing technologies, making CRISPR perfect for genome-wide screens. This guide...very close proximity to the region to be edited, making the PAM sequence (NGG) limiting. This PAM is abundant...inhibit CRISPR by a variety of mechanisms, including blocking crRNA loading or PAM recognition, inhibiting Cas9...sites is sufficient to repress transcription by blocking initiation. More robust transcriptional repression...repression through cytosine methylation with improved kinetics LSD1 — repression through targeted removal of ...proteins that target RNA instead of DNA enables tracking of RNA in live cells, including orthogonal RNA...

...exhibited high levels of constitutive activity, making them less than ideal. DREADDs Designer Receptors... previously used in human populations in Japan, making it an attractive option for translational studies...engineered to respond specifically to the anti-smoking drug varenicline. This PSAM carries mutations at... workflows (such as laser or LED illumination), making LMOs a versatile option. Coupling brighter bioluminescent...antipsychotic drugs: is M1 agonism a pre-requisite for mimicking clozapine's actions? Psychopharmacology, 178 (... PMID: 26889809 Slaviero, A. N., Gorantla, N., Simkins, J., Crespo, E. L., Ikefuama, E. C., Tree, M. O...

...necessary. For more information on cloning and working with plasmids, visit Addgene’s Molecular Biology...response in different ways, such as expressing cytokines and other immune-modulatory molecules. Another...occur with contact to mucous membranes or broken skin. The risk of exposure and reaction severity is increased... He, T. C., Zhou, S., da Costa, L. T., Yu, J., Kinzler, K. W., & Vogelstein, B. (1998). A simplified system... J., Sharff, K. A., Luu, H. H., Haydon, R. C., Kinzler, K. W., Vogelstein, B., & He, T. C. (2007). A protocol...