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  1. Retrovirus Guide

    Type
    Guide
    ...transgenes via increased nuclear export. LTR Subcomponents: U3 R U5 in cis LTR; Long terminal repeats; ...bigger than ∼3kb are packaged less efficiently. Subcomponents: U3; Unique 3'; region at the 3' end of viral...

  2. Plan Your Experiment

    Type
    Guide
    ...whether the edit was successful. PCR amplification, subcloning and Sanger sequencing (for HDR or NHEJ): Provides...PCR amplification of targeted region from DNA, subcloning into a plasmid, and screening individual clones...

  3. Lentiviral Guide

    Type
    Guide
    ...possible approaches to using this site include subcloning or appending compatible restriction sites onto... insert of interest using PCR. The process of subcloning consists of digesting the insert of interest ...

  4. Cloning

    Type
    Guide
    ...restriction enzyme sites. You can easily move (subclone) any piece of DNA that already has restriction...

  5. Chemogenetics Guide

    Type
    Guide
    ...targeted to specific tissues, cell types or even subcellular regions of a neuron. Cell-specific expression...

  6. Optogenetics Guide

    Type
    Guide
    ...blue-shifted channelrhodopsin from Platymonas subcordiformis 445 CoChR Channelrhodopsin from Chloromonas...
Showing: 101 - 106 of 106 results