We narrowed to 872 results for: cel

...molecular switches that transmit signals within the cell. GTPases have an active (GTP) and inactive (GDP)...prominent roles in signaling pathways regulating cell migration, survival, adhesion, growth, and differentiation...Cancer Impact Ras is ubiquitously expressed in all cells and all tissues. Ras can activate a variety of ...pathways, which ultimately turn on genes involved in cell growth, differentiation and survival. Ras is essential...essential for regulating the growth of the cell, however when mutations occur in the Ras gene it can be... active form, constantly activating pathways for cell growth and potentially leading to cancer. Constitutively...E2F2 E2F3 E2F transcription factor ECT2 Epithelial cell transforming 2 EGFR Epidermal growth factor receptor...

...Expression of firefly luciferase from a cell-free mammalian cell lysate Marcel Bruchez 108542 pLenti-EF1a-Luciferase-IRES-Blast-WPRE...not secreted, so cells must be lysed or the substrate must be delivered intracellularly to measure luminescence...expression of luciferase in the cytoplasm of mammalian cells Peter Cockerill 212936 pGL3 Basic Vector Firefly... viral infection efficiency, to study changes in cell physiology, or as a reference level of expression...Expression of Gaussia luciferase; GFP is expressed if cells are infected with virus Christopher Buck 98580 pLX304...membrane-anchored version useful for labeling extracellular vesicles. LumiScarlet : A fusion of LumiLuc ...serve as sensors of proteome stress. pHLuc : An extracellular pH sensor consisting of a pH-sensitive GFP (...

...then cell type. For CNS: For infant rhesus macaques: Broad tropism - AAV.MaCPNS1, AAV.MaCPNS2 Cell type-biased...tropism - AAV.CAP-B22 Cell type-biased: Neurons - AAV.CAP-B10 Brain vascular cells - AAV.CAP-Mac For rats...tropism - AAV-PHP.eB Cell type-biased: Neurons - AAV.CAP-Mac Brain vascular cells - AAV9-X1.1, AAV1-X1...including: the delivery method, mouse strain, target cell type or tissue of interest, and model organism under...serotype has been reported to occur by using the cellular receptor LY6A ( Huang et al., 2019 (Link opens... above helps you choose the best capsid for your cell type. First, do you want to target CNS ...Broad tropism - AAV-PHP.eB, AAV-PHP.AX, AAV-PHP.eC Cell type-biased: Astrocytes - AAV-PHP.AX with GRE or...

...proliferating cells. Expression may cause repetitive recombination leading to genomic instability and cell death...and control the expression of specific genes in cells or organisms. Each recombinase enzyme recognizes... the enzyme at specific times or locations using cell-specific promoters or inducible systems, you can...promoters. Expression of both fragments in the same cell reconstitutes a functional protein, allowing recombination...expression. For example, iCre or FLPo in mammalian cells may facilitate higher rates of recombination. Gene...Flp-IN: A single FRT site within the genome of a host cell line serves as the target location for integration...controls such as Cre-only lines to account for cellular toxicity in your system. Using inducible Cre systems...

...adipose tissue-derived mesenchymal stem cells into pluripotent stem cells by a mutant adeno-associated viral...serotype also demonstrates enhanced transduction of cells in culture for ex-vivo conditioning. The AAV2(trpYF...displays enhanced transduction of hematopoietic stem cells and microglia. The AAV6(dbY-F+T-V) serotype was ...AAV-mediated transduction and targeting of retinal bipolar cells with improved mGluR6 promoters in rodents and primates...human gene therapy of glaucoma and their reversed cell entry characteristics. Gene Ther . 2020 Apr;27(3...delivery of a novel AAV vector targets ON bipolar cells and restores visual function in a mouse model of... of Primary Human Hematopoietic Stem/Progenitor Cells by AAV6 Vectors: Strategies for Overcoming Donor-Variation...

...well-defined cellular sub-populations has generally been limited to single-component cellular definitions...to enable three-feature cell targeting. C) Example of viral targeting of cells expressing Cre AND Flp,...parameters. Figure 1: Examples of intersectional cell population definitions using recombinases. A) Cre...adeno-associated virus (AAV)-borne payloads to be expressed in cells based on a doubly-specified combination of genetic...multiple recombinase-expression strategies to define cellular sub-populations of interest, including dual-transgenic...Neve R, Huang ZJ, Deisseroth K. 2014. Targeting cells with single vectors using multiple-feature Boolean...Cortex Reveals Local and Long-Range Circuit Motifs. Cell Rep. 28(13):3450-3461. PubMed (Link opens in a new...

... Anti-Pan-Neurofascin (extracellular) [A12/18R] Pan-Neurofascin (extracellular) Rat Mouse IgG2a 177439...Anti-Kv1.4 K+ channel (extracellular) [L71/5R] Kv1.4 K+ channel (extracellular) Rat Mouse IgG2a 177489...Mouse IgG2a 177564 Anti-ADAM22 (extracellular) [N57/2R] ADAM22 (extracellular) Mouse Mouse IgG2a 177565 Anti-GluN2B...Anti-Kv1.4 K+ channel (extracellular) [L71/22] Kv1.4 K+ channel (extracellular) Rat Mouse IgG2a 190292...neo-epitope Human Mouse IgG2a 206584 Anti-Celf4/Brunol4 [N446/80R] Celf4/Brunol4 Human Mouse IgG2a 206585 Anti-Kv11.3...-Neurofascin (extracellular) scFv [A12/18] A12/18 scFv 2t Pan-Neurofascin (extracellular) Rat Mouse 190486...Kv1.4 K+ channel (extracellular) scFv [L71/5] L71/5 scFv Kv1.4 K+ channel (extracellular) Rat Mouse 190518...

...Laplante M, Sabatini DM. Cell. 2012 Apr 13;149(2):274-93. doi: 10.1016/j.cell.2012.03.017. PubMed PMID... (mTOR) is a key metabolic regulator controlling cell growth and proliferation. mTOR, a serine/threonine...signals to determine the nutritional state of the cell and regulates the switch from anabolic to catabolic...growth factors, including insulin. mTORC2 influences cell survival and cytoskeletal organization. Cancer Impact... promotes the conditions needed for uncontrolled cell growth and proliferation, including an abundant ...appears to signal upstream of Akt, also promotes cell survival and proliferation. mTOR Pathway Plasmids... one drug, many effects. Li J, Kim SG, Blenis J. Cell Metab. 2014 Mar 4;19(3):373-9. doi: 10.1016/j.cmet...

...complete plasmid can be transformed into bacterial cells for propagation. The majority of ligation reactions...Interpretation Uncut vector - Checks viability of competent cells and verifies the antibiotic resistance of the plasmid...

...Jinhyun Kim Tools for Cell Ablation These AAV encode tools that can be used for cell ablation. ID Name Expression...Transactivators Affinity Purification Neurophysiology Cell Ablation Genome Engineering Labeling Overexpression...expression of designer pro-taCasp3 and TEVp for studying cell ablation. 1, 5, 8 Nirao Shah , Jim Wells 124364 ...diphtheria toxin receptor fused to GFP for studying cell ablation. 2 Thomas Jessell , Eiman Azim 207997 pAAV...Tools These AAV encode tools that can be used for cell or protein labeling. ID Name Expression System Activity...AAV8-DIO-Reporter viruses infected in postsynaptic cells. 5, 8 Don Arnold 232352 pAAV-DIO-ATLASsnFlp Syn-...-fDIO-Reporter viruses infected in postsynaptic cells. 8 Don Arnold 232353 pAAV-DIO-BACE-HA Syn-driven...

...physiological intracellular processes with genetically encoded fluorescent biosensors. Subcellular Localization...Kits Allen Institute for Cell Science Plasmid Collection New England BioLabs Cell-Imaging Plasmid Collection...Localization Visualize organelles and subcellular structures with well-characterized marker proteins. In Vivo...activate, inhibit, and control molecular pathways or cells. Chemogenetics Guide | Chemogenetics Plasmids Use...activate or inhibit engineered receptors that affect cell signalling pathways. Luciferase Plasmids Use bioluminescent...

... location of your protein of interest within the cell, you will need to tag it with something that makes...fused to FPs and monitor their localization in live cells. ID Plasmid Tags PI Additional Addgene Protein Visualization... and expression species. The New England Biolabs Cell-Imaging Plasmid Collection contains plasmids for... inside living or fixed bacterial and mammalian cells. Addgene Blog A Guide to Selecting Fluorescent Dyes... Siouxsie Wiles 106476 pET28a_T5-ARG1 Bacterial cells Ultrasound (acoustic reporter gene, ARG1) Escherichia...Mikhail Shapiro 106473 pET28a_T7-ARG1 Bacterial cells Ultrasound (acoustic reporter gene, ARG1) Escherichia...Mikhail Shapiro 106474 pET28a_T7-ARG2 Bacterial cells Ultrasound (acoustic reporter gene, ARG2) Escherichia...

...integrase to insert the viral genome into a host cell. Scientists can take advantage of this to stably...integrate transgenes of interest into a variety of cell types. Wild-type gamma-retroviruses have been engineered...of Large T antigen for creation of immortalized cells. See the Weinberg Lab page for additional resistance...Expression of hTERT for creation of immortalized cells. See plasmid 1771 for puromycin selection, and plasmid...inducible expression of GFP T2A Cre fusion in mammalian cells. Floris Foijer 47916 pRXTN CMV/MSV A modified version...MoMSV Stable expression of luciferase in mammalian cells. Alice Wong 83356 pMXs-3XHA-EGFP-OMP25 MoMSV For...tagging mitochondria with HA epitopes in mammalian cells. David Sabatini Return to top Popular Gamma-Retroviral...

...applications of genetic code expansion in cell biology . Nat Rev Mol Cell Biol, 13 (3), 168–182. https://doi....expanding the genetic code in bacteria and mammalian cells. Plasmid...which means they work independently of the host cell's natural genetic machinery. The orthogonal aaRS ...while E. coli pairs are orthogonal in eukaryotic cells. Pairs from methanogens like Methanosarcina barkeri... are orthogonal both in bacteria and eukaryotic cells. Many of these sets have been mutated and screened... Once these plasmids have been introduced in the cell, the ncAA provided in the media can be incorporated...match the growth medium, ncAA concentration, and the cell lines used previously. Remember that the orthogonal...

...suitable for virus purification and isolation of cells, organelles, lipoproteins, and macromolecules. Importantly..., iodixanol is inert and non-toxic to mammalian cells. Therefore, and unlike other density gradient media...

...nucleotide variant lines in an isogenic cellular background. All cell lines and phenotypic datasets that result...CRISPR Guide CRISPR Protein Tagging Plasmids for Stem Cell Research Neurodegeneration Research Collection The...Plasmids The following plasmids can be used to create cell lines with endogenously-tagged gene variants or ...or to differentiate human iPSCs into other cell types, as noted in the purpose field: ID Plasmid Description...neurodegenerative diseases with genomic engineering: A new stem cell initiative from the NIH. Neuron . 2021 Apr 7;109...

...of our protocols supports reproducibility and accelerates science. Here, we list the specific equipment...shaker ELISA plate washer (optional) Scale Microsoft Excel or similar software Reagents Purified antigen Purified...

...p53 are in promoting cell cycle arrest, apoptosis, or senescence in damaged cells. The p53 name arose ...transcription factor and tumor suppressor activated by cellular stress. Cancer...transcription factor and tumor suppressor activated by cellular stresses like DNA damage, oxidative stress, and...or E2 Cyclin G CCNG1 CCNG2 Cyclin G1 or G2 CDC25 Cell division cycle 25C Cdk4/6 CDK4 CDK6 Cyclin-dependent...member 10b E2F-1 E2F transcription factor 1 Fas Fas cell surface death receptor Gadd45 GADD45A GADD45B GADD45G...

...collaborative NET research community is pivotal in accelerating progress in the diagnosis and treatment of neuroendocrine...and foundations, who share in their vision to accelerate scientific discovery that will help create new... Modeling Neuroendocrine Tumors Using Adult Stem Cell-Derived Organoids Matthew Meyerson Dana-Farber Cancer...Institute Epigenetic Regulators of Intestinal Endocrine Cells and Carcinoid Tumors Qiao Zhou Harvard University...University Epigenetic Regulators of Intestinal Endocrine Cells and Carcinoid Tumors Bradley Bernstein Broad Institute...

...important to normal cell signaling and physiology, and, when dysregulated, can enable cancer cell survival. Although...that is essential for regulating the growth of the cell. p53 p53, the most frequently mutated gene in human...and Libraries for Cancer Research Broad Target Accelerator Plasmids: Collection of vectors from the Boehm...mutant alleles described in the initial Target Accelerator publications, focused on somatic variants found...