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We narrowed to 976 results for: nin

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Showing: 101 - 120 of 976 results

  1. Synthetic Biology - Assembly Standards Guide

    Type
    Collection
    ...suffix sequences are given for the coding strand , meaning that the suffix will need to be reverse complimented...should be avoided whenever possible. Other popular cloning strategies for the assembly of synthetic parts ...systems such as MoClo . Check out our Molecular Cloning Techniques Guide for an overview of the most common...

  2. Antibody Production

    Type
    Collection
    ...concentration is confirmed by Coomassie staining. For Coomassie staining, the sample is separated via a denaturing...formulation buffer is phosphate-buffered saline containing 1 mM sodium azide. This buffer is not compatible...

  3. Viral Vector Guides and Plasmids

    Type
    Collection
    ...multi-plasmid systems. These systems consist of a plasmid containing the genetic information to be delivered (transfer... or shuttle plasmid) and one or more plasmids containing the necessary components of the viral genome ...Many viral plasmids are also self-inactivating, meaning that they can only complete one round of transcription...

  4. Allen Institute for Cell Science Plasmid Collection

    Type
    Collection
    ...Histones 109119 CTNNB1-mEGFP AICSDP-47 mEGFP Beta-catenin Adherens junctions 107579 RAB5A-mEGFP AICSDP-39...stress granules 114411 TNNI1-mEGFP AICSDP-34 mEGFP Troponin I, slow skeletal type Sarcomeric thin filaments...Factor 124607 ACTN2-mEGFP AICSDP-63 mEGFP Alpha-actinin-2 Sarcomeric z-disks 124608 NPM1-mTagRFP-T AICSDP...

  5. p53 Pathway

    Type
    Collection
    ...activating factor 1 ATM ATM serine/threonine kinase ATR ATR serine/threonine kinase B99 G-2 and S-phase expressed...PIRH-2 Ring finger and CHY zinc finger domain containing 1 (RCHY1); E3 ubiquitin protein ligase PTEN Phosphatase...

  6. Kazuhiro Oka Lentiviral Vectors

    Type
    Collection
    ...suited to simplify the cloning process as it can be used in blue–white screening . Adenoviral Vector ID...promoter. WPRE has been removed to increase the cloning capacity. pCDH-CB-IRES-copGFP-T2A-Puro 72299 Express...

  7. Adeno-associated virus (AAV) Plasmids

    Type
    Collection
    ...plasmids: A transfer plasmid (or cis plasmid) containing the transgene or genetic elements of interest...cells. A packaging plasmid (or Rep/Cap plasmid) containing the AAV replication (Rep) and capsid (Cap) genes...determine the AAV vector serotype. A helper plasmid containing adenoviral or herpesviral helper genes. These...

  8. Open Enzyme Collection

    Type
    Collection
    ...collection include polymerase chain reaction (PCR), cloning, isothermal amplification, reverse transcription...the basis of manufacturing their own enzymes by cloning them into an expression vector of their choice ...Jennifer Molloy Lab Plasmids Blog: Golden Gate Cloning...

  9. Lentivirus Plasmids

    Type
    Collection
    ....1 – TRC cloning plasmid 3rd U6-driven shRNA empty plasmid. Includes a stuffer for easy cloning. David ... the gene of interest. Can also express shRNA (cloning H1-shRNA cassettes into the unique SnaBI site in...

  10. Rinehart Lab Phosphoprotein Reagents

    Type
    Collection
    ...plasmid and is not compatible with other plasmids containing high copy ColE1/PUC-like origins. Therefore, ...at the central position. Introducing Hi-P for screening phosphorylation-dependent protein-protein interactions...domain of NEDD4-2 (Pooled Libraries #111705-8) . Screening kinases The Mode #1 phosphosite library can also...

  11. Cre-Lox and Other Site-Specific Recombinases

    Type
    Collection
    ...mediate the integration of a large DNA payload containing an attB target site into a genomic attP site....the expression of one gene while simultaneously turning on another. The FLEx switch system takes advantage... target location for integration of a plasmid containing a gene of interest and another FRT site. Flp ...

  12. KLF Research Plasmids

    Type
    Collection
    ...fibrosis, atherosclerosis, and carcinogenesis. The defining feature of the KLF family is the presence of three...resource on Addgene. If you have created plasmids containing KLF, we encourage you to contribute to this community...

  13. MAPK Plasmids

    Type
    Collection
    ...extracellular signal-regulated kinases (ERKs), are serine/threonine-specific protein kinases. They regulate diverse...collection for community. If you have created plasmids containing MAPK and would like to add to this collection...

  14. Plasmid Collections

    Type
    Collection
    ... view all kits. Kit Types Fluorescent Proteins Cloning Genome Engineering Pathways & ORFs Synthetic Biology...libraries. Pooled Library Types Barcoding CRISPR Screening Surface Display cDNA Depositor Collections A modular...

  15. CRISPR Plasmids - Cascade-Cas3

    Type
    Collection
    ...directions from the target region, effectively turning Cas3 into a DNA “shredder” system that can cause... a boundary defined by anti-CRISPR proteins or running into other proteins bound to DNA, and the break...

  16. Bikard Lab - CRISPR Repression Collection

    Type
    Collection
    ...Cui, L., Bikard, D., & Van Teeffelen, S. (2018). Tuning dCas9’s ability to block transcription enables ...Dodd, I. B., & Shearwin, K. E. (2013). One-Step cloning and chromosomal integration of DNA. ACS Synthetic...

  17. CRISPR Plasmids and Resources

    Type
    Collection
    ...background information on CRISPR and the basics for planning your first CRISPR experiment. CRISPR 101 eBook...libraries are a powerful tool for genome-wide screening. Purify dCas9 fused to an epitope tag can be used...

  18. Malate Dehydrogenase CUREs Community Collection

    Type
    Collection
    ...window) External Collaboration Results in Student Learning Gains and Positive STEM Attitudes in CUREs. Callahan...undergraduate research experiences (CURE) impacts student learning and attitudinal outcomes: A study of the Malate...

  19. New England Biolabs Cell-Imaging Plasmid Collection

    Type
    Collection
    ...Technology SNAP-tag SNAP-tag is based on the human O6-alkylguanine-DNA-alkyltransferase (hAGT), a DNA repair protein... but reacts with benzylcytosine rather than benzylguanine derivatives to allow for dual labeling and visualization...

  20. Promega Plasmid Collection

    Type
    Collection
    ...sensitive gene expression analysis and fusion vectors containing tags such as NanoLuc, HaloTag, SmBiT, and LgBiT...editing outcomes, and conducting high-throughput screening in drug discovery. NanoLuc Fusions NanoLuc (Link...
Showing: 101 - 120 of 976 results