... shRNA under the mouse U6 promoter; A CMV-EGFP reporter cassette is included in the vector to monitor ...

...promoter’ in the tables for species-specific tools). Support open science in plant biology - deposit plant plasmids...

... of p53’s pro-apoptotic effects is especially important to tumorigenesis. Inheriting only one functional...

...on each item's material page. Actual titers are reported with each shipment. For more details about our...

...for long term studies) while other viruses are temporary in the host. Use this guide to learn more about...

...function. Browse plasmids or viral preps created with support from the BRAIN Armamentarium Project below. Plasmids...

...with the following article: Natsume, et al. Cell Reports 2016 Förstemann Drosophila Cell Tagging System ...

...TALENs for Endogenous Zebrafish Gene Targets Recent reports have shown that engineered TALENs can be used to...

...Cong et al. , Science 2013 for more details. One important note is that this ‘split RNA’ design is not as...

...tropism exhibited by the PHP.eB serotype has been reported to occur by using the cellular receptor LY6A (...

...libraries can be used to identify genes that are important to a given phenotype. Current libraries are available...

...nucleolytically inactive Cas9-VP64 fusion A sgRNA incorporating two MS2 RNA aptamers at the tetraloop and stemloop...

...pyogenes 23929339 Sheen pGL3-Basic-8x-gRNA-eGFP reporter synthetic AAAGGTCGAGAAACTGCAAA 60719 activate ...

...be copied (amplified) by bacteria, which is an important characteristic of why plasmids are convenient ...would not survive antibiotic treatment. It is important to distinguish that the antibiotic resistance ...promoter). The strength of the promoter is also important for controlling the level of insert expression...information on CRISPR check out Addgene’s CRISPR guide . Reporter Plasmids - Used for studying the function of genetic... genetic elements. These plasmids contain a reporter gene (for example, luciferase or GFP) that offers...certain diseases. Sequencing DNA is extremely important when verifying plasmids to ensure each plasmid...vitro DNA replication but relies on the random incorporation of modified, fluorescently tagged bases onto...

...and systems. When planning your experiment, the important component to consider and optimize is the transfer...Cre-based regulation and fluorescent fusions or reporters. Browse lentivirus plasmids available from Addgene...order to create functional lentiviral particles. IMPORTANT : A 3rd generation transfer plasmid can be used...cells, they kill off any cells that have not incorporated the lentiviral genome and those cells that survive...expanded to create stable cell lines, which have incorporated the lentiviral genome and harbor the genetic...interest and contains the sequences that will incorporate into the host cell genome, but cannot produce...transcription of the full-length virus after it has incorporated into a host cell. The potential for oncogenesis...

...neural populations at distinct times. Temporal considerations. Temporal precision is key in optogenetic experiments... exposed to light of the correct wavelength, the pore opens, cations flow into the cell (yellow dots),...optical switches Sensors are genetically-encoded reporters of molecular signals; e.g., calcium indicators...specific subsets of neurons, allowing precise spatiotemporal control of these neurons by turning on and ...addition of trafficking signal from Kir2.1 and ER export signal provide improved membrane targeting 589 ...the vesicular gamma aminobutyric acid (GABA) transporter (VGAT) and ChR2 is thus expressed in all GABAergic...photoswitchable” proteins offer exquisite spatial and temporal control of protein activity. Phytochrome, cryptochrome...

...and a ssDNA-fluorescently quenched reporter is added. As this reporter is degraded by Cas12a, it will release... using a fluorescent reporter. In SHERLOCK, a quenched ssRNA fluorescent reporter is added to the reaction...insertions. When designing the donor template, incorporating mutations that prevent further Cas9 cleavage... HDR efficiency. In most applications, a large portion of the Cas9-induced DSBs will still be repaired...the desired HDR-edited allele. Therefore, it is important to confirm the presence of the desired edit experimentally...pairs to the 3’ flap, and the desired edit is incorporated into the DNA by reverse transcription. The edited...applications where orientation of the insert is important. Type V-K CASTs appear to exhibit almost unidirectional...

...promoter is typically used for gRNA May contain reporter gene (e.g. GFP) to identify and enrich positive...vector or separate transfer vectors May contain reporter gene (e.g. GFP) or selection marker to identify... to target cells through microinjection or electroporation Transient expression of CRISPR components Expression...may alter protein function when they occur in important protein domains. For gene editing experiments ... addition to off-target activity , it is also important to consider factors that maximize cleavage of ...25408407 Hashimoto, M., & Takemoto, T. (2015). Electroporation enables the efficient mRNA delivery into the...facilitates CRISPR/Cas9-based genome editing. Scientific Reports . 5 , 11315. PMID: 26066060 Zuris, J. A., Thompson...

...necessary in cases where extreme specificity is important. Their epitope specificity allows binding to a...antibodies only bind a single epitope, so it is important to choose a monoclonal antibody that will recognize...using a secondary against a tag, such as His, incorporated in the sdAb. sdAbs can have weak signals due...with conjugating reactions are possible. It is important to research your reaction and buffer before conjugating... the basic steps needed for each application, important controls, and any special considerations to think...which are bacterial proteins that bind to the Fc portion of an antibody. However, they are not suitable ... assay, selection of the fluorophores used is important. It is best to inversely pair the brightness of...

...control expression of a chemogenetic reporter ensures that the reporter remains silent until a cell or tissue-specific...ligands can have high potency, which is potentially important for dosing in animal studies, made them attractive...agonists for PSAM4. A given PSAM is coupled with an ion pore domain (IPD) to form ligand-gated ion channels (...signaling. PSAM-based LGICs can be classified by the ion pore domain they are coupled to: PSAM-Gly LGICs pair ... of PSAMs and their activity in neurons PSAM Ion Pore Domain Ligand(s) Effect Outcome (in neurons) Reference...