We narrowed to 916 results for: tro
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TypeCollection...ESRL2, NR3B2 estrogen-related receptor beta ESRRG Plasmids ESRRG, ERR3, ERRgamma, NR3B3 estrogen-related receptor...ESR2, ER-BETA, ESR-BETA,ESRB, ESTRB, Erb, NR3A2 estrogen receptor 2 (ER beta) ESRRA Plasmids ESRRA, ERR1...ERR1, ERRa, ERRalpha, ESRL1, NR3B1 estrogen-related receptor alpha ESRRB Plasmids ESRRB, DFNB35, ERR2, ...
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CRISPR Plasmids - Yeast
TypeCollection... Cut Fully functional CRISPR/Cas enzymes will introduce a double-strand break (DSB) at a specific location...frameshifts, creating loss of function alleles. To introduce specific genomic changes, researchers use ssDNA...PI Publication Nick CRISPR/Cas nickase mutants introduce gRNA-targeted single-strand breaks in DNA instead...mutants can also be used with a repair template to introduce specific edits via homology-directed repair (HDR... -
Chemogenetics AAV Preps
TypeCollection...filtering options. *AAVrg = retrograde serotype, produced with the AAV retro helper plasmid from the Karpova... dependent) Serotype AAV1 AAV2 AAV5 AAV8 AAV9 Retrograde AAV PHPeB Clear Filters ID Name Receptor and ...Karpova and Schaffer labs. See the Retrograde AAV Inventory Page . Browse all Chemogenetics AAV Don’t ... -
Microbiology Resources
TypeCollection... cerevisiae - Dueber Lab Yeast Prototrophy : Restoration of prototrophy in common S. cerevisiae lab strains...Methanococcus maripaludis Methanothermobacter thermautotrophicus Sulfolobus islandicus Plasmids for Bacteria...Resources Read our CRISPR Guide for a general introduction to CRISPR technology. Check out our CRISPR reference... -
Plant Plasmids and Resources
TypeCollection...includes a high efficiency intron-optimized SpCas9-coding gene, zCas9i, and an intronized Cas9 series of vectors...refer to Addgene's CRISPR Guide for a general introduction to CRISPR technology or the Plant CRISPR Resources... and experimental resources that relate to the control of gene expression in the grasses. OpenPlant Synthetic... -
CRISPR Plasmids - Bacteria
TypeCollection... Cut Fully functional CRISPR/Cas enzymes will introduce a double-strand break (DSB) at a specific location...frameshifts, creating loss of function alleles. To introduce specific genomic changes, researchers use ssDNA...PI Publication Nick CRISPR/Cas nickase mutants introduce gRNA-targeted single-strand breaks in DNA instead...mutants can also be used with a repair template to introduce specific edits via homology-directed repair (HDR... -
CRISPR Plasmids - Mammalian Expression
TypeCollection... Cut Fully functional CRISPR/Cas enzymes will introduce a double-strand break (DSB) at a specific location...frameshifts, creating loss of function alleles. To introduce specific genomic changes, researchers use ssDNA...PI Publication Nick CRISPR/Cas nickase mutants introduce gRNA-targeted single-strand breaks in DNA instead...mutants can also be used with a repair template to introduce specific edits via homology-directed repair (HDR... -
CRISPR Plasmids - Plants
TypeCollection... Cut Fully functional CRISPR/Cas enzymes will introduce a double-strand break (DSB) at a specific location...frameshifts, creating loss of function alleles. To introduce specific genomic changes, researchers use ssDNA...PI Publication Nick CRISPR/Cas nickase mutants introduce gRNA-targeted single-strand breaks in DNA instead...mutants can also be used with a repair template to introduce specific edits via homology-directed repair (HDR... -
University of Florida Serotype Testing Panel for the Eye and Brain
TypeCollection... vectors undergo quality control, including AAV titration by ddPCR, in vitro and (when possible) in vivo...chloride, 20 mM Tris at pH 8.0) Suitable for in vitro as well as in vivo delivery where the vector is ...pTR-UF11 chimeric CMV/Chicken Beta actin (CBA) GFP Control Sergei Zolotukhin Citation Information AAV2(Y444F... -
Rinehart Lab Phosphoprotein Reagents
TypeCollection...phosphoserine in >36,000 phosphosites by tandem mass spectrometry (Barber et al, Nat. Biotech. 2018). This phosphosite...supD tRNA ( 68307 ) at the central position. Introducing Hi-P for screening phosphorylation-dependent ...interactions. The phosphosite DNA library can also be introduced into a second expression vector encoding a split...human substrates of kinases of interest by mass spectrometry in a technique called serine-oriented library... -
Zhang Lab CRISPR Page
TypeCollection...recombinase, and Kras G12D HDR template 60225 : control for AAV-KPL; sgRNA targeted to LacZ, plus luciferase...within the mouse genome. This plasmid is used as a control for AAV-KPL. #60226 - AAV:ITR-U6-sgRNA(backbone...targeting the neuronal-specific gene NeuN. As a control they designed an sgRNA targeting LacZ, which is... L, Heidenreich 1, Banerjee A, Habib N, Li Y, Trombetta J, Sur M, Zhang F. Nat Biotechnol . 2015 Jan;33... -
Feng Zhang Multiplexed Overexpression of Regulatory Factors (MORF) Collection
TypeCollection... 1,836 genes encoded by 3,548 isoforms and two control vectors. The individual clones, or the pooled library...Abudayyeh OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F. Cell 2023 Jan 5; 186(1)...reading frames with or without GFP and mCherry controls. Do you have suggestions for other plasmids that... -
CRISPR Plasmids - Double-Strand Break (Cut)
TypeCollection...Posts Fully functional CRISPR/Cas enzymes will introduce a double-strand break (DSB) at a specific location...frameshifts, creating loss of function alleles. To introduce specific genomic changes, researchers use ssDNA... tables below for CRISPR plasmids designed to introduce a DSB. Plasmids are available for expression in... -
DNA Service - Cloning Grade DNA
TypeCollection...find current pricing information Standard Quality Control Plasmid verified by next generation sequencing ...Plasmid Description Industry PI FAQ What Quality Control analyses are performed on my cloning grade DNA ... and 260/280 ratio has been determined by spectrophotometer. Other methods of quantification may yield... -
Bikard Lab - CRISPR Repression Collection
TypeCollection...blocks the progression of the RNA polymerase, thus strongly decreasing the expression level. Mismatches between...target sequence lower the repression level. By controlling the number of mismatches we can obtain a range...reporters, mCherry and sfgfp in this case, can be controlled using a plasmid‐borne CRISPR array coding for... -
Viral Prep Service for Plasmids and Pooled Libraries
TypeCollection...production process and quality control, with examples of quality control data. Help Center Articles Additional...various aspects of viral vectors, including troubleshooting advice and explanations of viral vector technologies... -
Fluorescent Protein Guide: Subcellular Localization
TypeCollection...Filaments Utrophin (aa# 1-261) GFP William Bement 26740 mCherry-UtrCH Actin Filaments Utrophin mCherry ...cycle) Cep170 EGFP Erich Nigg 41163 pEGFP PICH Centromeres (dependent on cell cycle) PICH EGFP Erich Nigg...EGFP Ken-Ichi Takemaru 54234 mEmerald-PLK1-N-16 Centrosome Plk1 mEmerald* Michael Davidson 54491 mCherry-Lifeact... -
Mammalian RNAi Tools
TypeCollection...plasmids are in a lentiviral backbone, with some retroviral and AAV backbone options. Plasmids for constitutive...protocols Lentiviral packaging plasmids Web Resources Trono Lab Lentivectors Toolbox Optimized shRNA design ... in a new window) . Wiznerowicz, M., Szulc, J., Trono, D. (2006). Tuning silence: conditional systems ... -
mTOR Pathway
TypeCollection...apamycin (mTOR) is a key metabolic regulator controlling cell growth and proliferation. mTOR, a serine...in cancer promotes the conditions needed for uncontrolled cell growth and proliferation, including an ...Kris Wood) References mTOR signaling in growth control and disease. Laplante M, Sabatini DM. Cell. 2012... -
Worm Expression Resources
TypeCollection...CRISPR Read Addgene's CRISPR Guide for a general introduction to CRISPR technology. Addgene's worm CRISPR ...silencing in the germline. A "FLP-Out" system for controlled gene expression in Caenorhabditis elegans. - ...Hubbard Lab. Plasmids for the temporal and spatial control of gene expression in C. elegans by combining expression...