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We narrowed to 124 results for: yeast expression

Showing: 101 - 120 of 124 results

  1. CRISPR Plasmids - C. elegans

    Type
    Collection
    ...choose does not also express a gRNA, you will need to use a separate gRNA expression plasmid to target the...Promoter PI Publication Empty gRNA Expression Vectors Select a gRNA expression plasmid based on factors such... Mammalian Bacteria Drosophila Plant C. elegans Yeast Zebrafish Xenopus CRISPR Resources CRISPR Guide ...cloning method. When using CRISPR, you will need to express both a Cas protein and a target-specific gRNA in...On the other hand, gRNA plasmids that do not co-express a Cas protein can be paired with a wide variety...Promoter Cloning Enzyme(s) Delivery Resistance Co-expressed Cas9 Depositing lab Cas9 species = S. pyogenes...

  3. Cloning

    Type
    Guide
    ...protocol ) Back to Top Yeast-mediated Cloning and Oligonucleotide Stitching Yeast-mediated cloning is very...them into plasmids for replication and protein expression. So how do scientists recombine DNA? There are...majority of plasmids used for DNA cloning and expression contain several popular restriction enzyme sites...advantage of the powerful recombination abilities of yeast. Similar to Gibson, this method can efficiently ...accomplish this, you just need to introduce into the yeast the two (or more) fragments of DNA that you would...be set up to grow, transform and purify DNA from yeast. Back to Top...

  4. Institute for Protein Innovation

    Type
    Collection
    ...tags, as well as antibody plasmids to create or express plasmid-based antibodies. The majority of antibodies... platform (Link opens in a new window) based on yeast display technology (Link opens in a new window) ...The following plasmids can be used to create or express plasmid-based antibodies. ID Plasmid Description...

  5. CRISPR Plasmids - Drosophila

    Type
    Collection
    ...choose does not also express a gRNA, you will need to use a separate gRNA expression plasmid to target the...Promoter PI Publication Empty gRNA Expression Vectors Select a gRNA expression plasmid based on factors such... Mammalian Bacteria Drosophila Plant C. elegans Yeast Zebrafish Xenopus CRISPR Resources CRISPR Guide ...cloning method. When using CRISPR, you will need to express both a Cas protein and a target-specific gRNA in...On the other hand, gRNA plasmids that do not co-express a Cas protein can be paired with a wide variety...Promoter Cloning Enzyme(s) Delivery Resistance Co-expressed Cas9 Depositing lab Cas9 species = S. pyogenes...

  6. Adenovirus Plasmids

    Type
    Collection
    ...kb), high titers, and high levels of transgene expression. Additionally, these viruses are able to target...Disadvantages of adenoviruses include transient transgene expression, as well as the substantial immune response induced...cells or microorganisms, including bacteria and yeast. Two plasmids, termed a shuttle plasmid and an adenoviral...Block 6 with deletion in E3 and insertion of GFP expression cassette. Bunz 179202 pAd5-B6/7 ∆E3-GFP Adenoviral...Block 1 with deletion in E1 and insertion of GFP expression cassette. Bunz 16402 pShuttle Shuttle For production...For production of viruses with multicistronic expression + Venus tag; See plasmid 62622 for plasmid without...

  7. Antibody Plasmid Collection

    Type
    Collection
    ...components into plasmids and expressing these in bacteria, mammalian cells, yeast, plants, or insect cells... a single mammalian expression vector A tool kit for rapid cloning and expression of recombinant antibodies...scFv expression, which circumvents the problem of in-frame amber codons in scFvs A new expression vector... trusted partner labs. Learn More Cloning and Expressing Antibodies Antibody Plasmids Antibodies are large...length recombinant monoclonal antibodies (R-mAbs) expressed in mammalian cells. Monoclonal antibodies are ...applications in our Antibody Guide ! Cloning and Expressing Antibodies The following table highlights plasmid...plasmid collections that can be used to create and express plasmid-based antibodies. Description Article PI...

  8. CRISPR Plasmids - Parasites

    Type
    Collection
    ...CRISPR expression plasmids that have been designed for use in parasites.... Mammalian Bacteria Drosophila Plant C. elegans Yeast Zebrafish Xenopus CRISPR Resources CRISPR Guide ...

  9. Plasmids 101: Using Transposons in the Lab

    Type
    Blog Post
    ...Non-autonomous transposons, however, need another TE to express a transposase or reverse transcriptase so they ...size and is active in vitro as well as in vivo in yeast, plant, insect, and mammalian cells, including human...

  10. CRISPR Plasmids - gRNAs

    Type
    Collection
    ...gRNA expression vectors available from Addgene.... Mammalian Bacteria Drosophila Plant C. elegans Yeast Zebrafish Xenopus CRISPR Resources CRISPR Guide ...

  11. CRISPR References and Information

    Type
    Collection
    ... sgRNA dual expression: pAC2 , pAC152 , pAC153 , pAC154 ; pmax dCas9 activator expression: pAC91 , pAC92... support for bacteria ( E. coli, B. subtilis ), yeast ( S. cerevisiae ), worm ( C. elegans ), fruit fly...genomes are human (hg19 & hg38), mouse (mm10), and yeast (strain w303). Developed by Venetia Pliatsika and...design and cloning All-in-one plasmid containing expression cassette for NmCas9 and BsmBI site for cloning...pSIR-neo) , GFP (pSIR-GFP) , DsRed (pSIR-DsRed-Express2) , human CD2 (pSIR-hCD2) PDF, 108 KB Fujii iChIP...

  12. 22 Hot Plasmid Technologies from 2014

    Type
    Blog Post
    ...Cre-based Brainbow-expressing cells. Autobow plasmids are all-in-one versions which express self-excising ...multiple host cells in order to optimize expression. The expression plasmids for a given species are based...pMitoTimer from Yan’s lab, which is constitutively expressed, reports on the balance of biogenesis and mitochondrial...channel fluorescence images of C2C12 cells stably expressing pTRE-Tight-MitoTimer and rtTA. The cells were...down your endogenous protein of interest and co-expressing its replacement recombinant protein with a FKBP...deposited to Addgene, including lentiviral and AAV expression vectors for certain variants. Klapoetke et ... relatively long life-cycle of a protein from expression to degradation. Chemical approaches may require...

  13. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ...sensor for Yeast (AT1.03 derived) Dynamic analysis of cytosolic glucose and ATP levels in yeast using optical...Osmolarity SED1 osmolarity biosensor, for expression in E. coli , yeast, or plants Intrinsically disordered ...Kim , GENIE Project Calcium Bicistronic vector expressing GCaMP6 and mRuby2 for imaging neural activity... Huebener , Tobias Rose Calcium Soma-targeted expression of jGCaMP8f, GCaMP7f, or GCaMP6f Precision Calcium...6. Edward Boyden Calcium Intersectional viral expression of GCaMP6m/s in cells depdent on Cre, Flp, or...Karl Deisseroth , INTRSECT 2.0 Project Calcium Expression of GCaMP6f in PV cortical interneurons under ... Aug 4;11(1):3881. Adam Cohen Calcium GCaMP6f expression in forebrain GABA-ergic interneurons under the...

  14. CRISPR Plasmids - Bacteria

    Type
    Collection
    ...choose does not also express a gRNA, you will need to use a separate gRNA expression plasmid to target the...using does not also express a gRNA, you will need to use a separate gRNA expression plasmid to target the...j.bbrc.2013.08.013. Empty gRNA Expression Vectors Select a gRNA expression plasmid based on factors such... Mammalian Bacteria Drosophila Plant C. elegans Yeast Zebrafish Xenopus CRISPR Resources CRISPR Guide ...repressor peptide like KRAB, can knock down gene expression by interfering with transcription. Design your...cloning method. When using CRISPR, you will need to express both a Cas protein and a target-specific gRNA in...On the other hand, gRNA plasmids that do not co-express a Cas protein can be paired with a wide variety...

  15. CRISPR Plasmids - Mammalian Expression

    Type
    Collection
    ... CRISPR Mammalian Expression CRISPR Plasmids: Mammalian Expression The following CRISPR plasmids...choose does not also express a gRNA, you will need to use a separate gRNA expression plasmid to target the...using does not also express a gRNA, you will need to use a separate gRNA expression plasmid to target the...plasmids have been designed for use in mammalian expression systems. Cut Fully functional CRISPR/Cas enzymes... Mammalian Bacteria Drosophila Plant C. elegans Yeast Zebrafish Xenopus CRISPR Resources CRISPR Guide ...repressor peptide like KRAB, can knock down gene expression by interfering with transcription. Design your... is tagged with a biotin acceptor site and co-expressed with BirA biotin ligase. The locus is isolated...

  16. CRISPR Plasmids - Plants

    Type
    Collection
    ...choose does not also express a gRNA, you will need to use a separate gRNA expression plasmid to target the...using does not also express a gRNA, you will need to use a separate gRNA expression plasmid to target the...Marker PI Publication Empty gRNA Expression Vectors Select a gRNA expression plasmid based on factors such...CRISPR expression plasmids that have been designed for use in plants. CRISPR... Mammalian Bacteria Drosophila Plant C. elegans Yeast Zebrafish Xenopus CRISPR Resources CRISPR Guide ...repressor peptide like KRAB, can knock down gene expression by interfering with transcription. Design your...cloning method. When using CRISPR, you will need to express both a Cas protein and a target-specific gRNA in...

  17. TALEN Plasmids and Kits

    Type
    Collection
    ... that can be used to custom repress gene expression in yeast. TALORs consist of the DNA-binding domain...-inducible expression. pTAL6-BB contains the TEF1 promoter, giving constitutive expression of TALORs built...Golden Gate cloning method. Optimized for human expression. Feng Zhang LIC TAL Effector Assembly Kit Assembly...than the parental vectors. pC-GoldyTALEN directs expression of TALENs from a truncated CAGs promoter. RCIscript-GoldyTALEN...β-globin UTRs are included in the vector to enhance expression of the message. Both vectors utilize homodimeric...positions 1214–2210 of pTAL3 were cloned into a pCS2 expression vector resulting in shorter N- and C-terminal...constructs were designed for optimal mammalian expression of Voytas Golden Gate-assembled TALEN, both in...

  18. CRISPR Plasmids - Tagging

    Type
    Collection
    ...Plasmids: pX330S-2-PITCh - expresses Cas9 & PITCh-gRNA pX330A-FBL/PITCh - expresses Cas9, PITCh-gRNA, & FBL-specific... Mammalian Bacteria Drosophila Plant C. elegans Yeast Zebrafish Xenopus CRISPR Resources CRISPR Guide ...has been adapted to allow tagging of proteins expressed from their natural chromosomal context. These ....1)_No_FLAG_AAVS1_T2 (Addgene #79888) , which expresses an untagged Cas9 AND a gRNA targeting the AAVS1... and donor vectors as well as pX330S-2-PITCh, expressing a generic gRNA targeting the PITCh donor plasmid... the remaining 5 plasmids include Cre and FLP expression vectors, a general cloning plasmid, and a prebuilt...Drosophila cells that uses PCR to generate both an expression cassette for the Cas9-programming sgRNA and HR...

  19. COVID-19 Resources

    Type
    Collection
    ... SARS-CoV-2 plasmids for E. coli , yeast, and mammalian expression, and spike protein...Browse the tables below to find popular plasmids expressing genes encoding SARS-CoV-2 proteins, (spike protein...and codon-optimized versions designed for your expression system. Some of the following plasmids are available...Collection – This page includes a table of all spike expression plasmids in our collection that are suitable ...Neutralization Response Against Pseudoviruses Expressing SARS-CoV-2 Spike Protein Variants Return to top...

  20. Genetic Code Expansion

    Type
    Collection
    ...ttRNA pair (knocked out trpS and trpT genes). Expresses yeast TrpRS/tRNA pair instead. For directed evolution...plasmids, as depicted in the figure below: A plasmid expressing the tRNA and its cognate aminoacyl-tRNA-synthetase...codon is often used. The gene of interest can be expressed from a plasmid containing a UAG codon at the place...with a different amino acid. They are typically expressed from a single plasmid, with multiple copies of...make sure that these conditions are true. Always express first with a control reporter gene – GFP for E....mCherry-GFP for mammalian cells. You should also express the protein with and without the ncAA in the media...
Showing: 101 - 120 of 124 results