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We narrowed to 130 results for: yeast expression

Showing: 101 - 120 of 130 results

  1. Depositor Collections

    Type
    Collection
    ...Plasmid Collection Botman-Teusink Yeast FP Collection Protein Expression Cell Migration Consortium Malate...to drive selective expression in the brain - Simpson Reagents for improved expression of recombinant phosphoproteins...CRISPR, disease research, visualization, protein expression, synthetic biology, and viral vector plasmids...Open Enzyme Collection Screening Multiplexed Overexpression of Regulatory Factors (MORF) Collection Synthetic...

  2. Ginkgo Bioworks COVID-19 Collection

    Type
    Collection
    ... enable researchers to express SARS-CoV-2 viral proteins in several expression systems. Addgene has partnered...nsp3, OC43, NL63, HKU1, and 229E) Expression type (e.g. bacterial, yeast, or mammalian) Tags (e.g. 6xHis...to Industry page. These plasmids have not been expressed or functionally validated by Ginkgo or Addgene... V5) Mutations Wild type (wt) Codon-optimized expression (recode) Truncated for making pseudovirus (Ctrunc...Shared by Addgene: SARS-CoV-2 Plasmids for Many Expression Systems for a summary of the Ginkgo collection...

  3. Plasmids 101: Using Transposons in the Lab

    Type
    Blog Post
    ...Non-autonomous transposons, however, need another TE to express a transposase or reverse transcriptase so they ...size and is active in vitro as well as in vivo in yeast, plant, insect, and mammalian cells, including human...

  4. CRISPR Plasmids - Xenopus

    Type
    Collection
    ...Promoter PI Publication Empty gRNA Expression Vectors Select a gRNA expression plasmid based on factors such... Mammalian Bacteria Drosophila Plant C. elegans Yeast Zebrafish Xenopus CRISPR Resources CRISPR Guide ...break (DSBs) at a specific location based on a co-expressed gRNA-defined target sequence. DSBs are preferentially...cloning method. When using CRISPR, you will need to express both a Cas protein and a target-specific gRNA in...On the other hand, gRNA plasmids that do not co-express a Cas protein can be paired with a wide variety...Promoter Cloning Enzyme(s) Delivery Resistance Co-expressed Cas9 Depositing lab Cas9 species = S. pyogenes...

  5. CRISPR Plasmids - Zebrafish

    Type
    Collection
    ...Promoter PI Publication Empty gRNA Expression Vectors Select a gRNA expression plasmid based on factors such... Mammalian Bacteria Drosophila Plant C. elegans Yeast Zebrafish Xenopus CRISPR Resources CRISPR Guide ...cloning method. When using CRISPR, you will need to express both a Cas protein and a target-specific gRNA in...On the other hand, gRNA plasmids that do not co-express a Cas protein can be paired with a wide variety...Promoter Cloning Enzyme(s) Delivery Resistance Co-expressed Cas9 Depositing lab Cas9 species = S. pyogenes...

  6. 22 Hot Plasmid Technologies from 2014

    Type
    Blog Post
    ...Cre-based Brainbow-expressing cells. Autobow plasmids are all-in-one versions which express self-excising ...multiple host cells in order to optimize expression. The expression plasmids for a given species are based...pMitoTimer from Yan’s lab, which is constitutively expressed, reports on the balance of biogenesis and mitochondrial...channel fluorescence images of C2C12 cells stably expressing pTRE-Tight-MitoTimer and rtTA. The cells were...down your endogenous protein of interest and co-expressing its replacement recombinant protein with a FKBP...deposited to Addgene, including lentiviral and AAV expression vectors for certain variants. Klapoetke et ... relatively long life-cycle of a protein from expression to degradation. Chemical approaches may require...

  7. CRISPR Plasmids - C. elegans

    Type
    Collection
    ...choose does not also express a gRNA, you will need to use a separate gRNA expression plasmid to target the...Promoter PI Publication Empty gRNA Expression Vectors Select a gRNA expression plasmid based on factors such... Mammalian Bacteria Drosophila Plant C. elegans Yeast Zebrafish Xenopus CRISPR Resources CRISPR Guide ...cloning method. When using CRISPR, you will need to express both a Cas protein and a target-specific gRNA in...On the other hand, gRNA plasmids that do not co-express a Cas protein can be paired with a wide variety...Promoter Cloning Enzyme(s) Delivery Resistance Co-expressed Cas9 Depositing lab Cas9 species = S. pyogenes...

  8. Institute for Protein Innovation

    Type
    Collection
    ...tags, as well as antibody plasmids to create or express plasmid-based antibodies. The majority of antibodies... platform (Link opens in a new window) based on yeast display technology (Link opens in a new window) ...The following plasmids can be used to create or express plasmid-based antibodies. ID Plasmid Description...

  9. CRISPR Plasmids - Cascade-Cas3

    Type
    Collection
    ... Mammalian Bacteria Drosophila Plant C. elegans Yeast Zebrafish Xenopus CRISPR Resources CRISPR Guide ...getting all components of the Cascade complex to express equally. To combat this, some CRISPR-Cas3 plasmids...Cascade-Cas3 plasmids. Plasmids are available for expression in mammalian systems, bacteria, and plants. Mammalian...

  10. CRISPR Plasmids - Drosophila

    Type
    Collection
    ...choose does not also express a gRNA, you will need to use a separate gRNA expression plasmid to target the...Promoter PI Publication Empty gRNA Expression Vectors Select a gRNA expression plasmid based on factors such... Mammalian Bacteria Drosophila Plant C. elegans Yeast Zebrafish Xenopus CRISPR Resources CRISPR Guide ...cloning method. When using CRISPR, you will need to express both a Cas protein and a target-specific gRNA in...On the other hand, gRNA plasmids that do not co-express a Cas protein can be paired with a wide variety...Promoter Cloning Enzyme(s) Delivery Resistance Co-expressed Cas9 Depositing lab Cas9 species = S. pyogenes...

  11. Adenovirus Plasmids

    Type
    Collection
    ...kb), high titers, and high levels of transgene expression. Additionally, these viruses are able to target...Disadvantages of adenoviruses include transient transgene expression, as well as the substantial immune response induced...cells or microorganisms, including bacteria and yeast. Two plasmids, termed a shuttle plasmid and an adenoviral...Block 6 with deletion in E3 and insertion of GFP expression cassette. Bunz 179202 pAd5-B6/7 ∆E3-GFP Adenoviral...Block 1 with deletion in E1 and insertion of GFP expression cassette. Bunz 16402 pShuttle Shuttle For production...For production of viruses with multicistronic expression + Venus tag; See plasmid 62622 for plasmid without...

  12. Antibody Plasmid Collection

    Type
    Collection
    ...components into plasmids and expressing these in bacteria, mammalian cells, yeast, plants, or insect cells... a single mammalian expression vector A tool kit for rapid cloning and expression of recombinant antibodies...scFv expression, which circumvents the problem of in-frame amber codons in scFvs A new expression vector... trusted partner labs. Learn More Cloning and Expressing Antibodies Antibody Plasmids Antibodies are large...length recombinant monoclonal antibodies (R-mAbs) expressed in mammalian cells. Monoclonal antibodies are ...applications in our Antibody Guide ! Cloning and Expressing Antibodies The following table highlights plasmid...plasmid collections that can be used to create and express plasmid-based antibodies. Description Article PI...

  13. CRISPR Plasmids - RNA Targeting

    Type
    Collection
    ... Mammalian Bacteria Drosophila Plant C. elegans Yeast Zebrafish Xenopus CRISPR Resources CRISPR Guide ...targeting plasmids. Plasmids are available for expression in mammalian systems, bacteria, and plants. Mammalian...

  14. COVID-19 Resources

    Type
    Collection
    ...collection contains: Plasmids for E. coli and Yeast expression Spike protein constructs for making VSV pseudo...SARS-CoV-2 infection. General Tools : Plasmids expressing Cas12a/Cas13a, Reverse Transcriptases, etc. that...Collection - This page includes a table of all spike expression plasmids in our collection that are suitable ... Collection page. SARS-CoV-2 Pooled Libraries - Yeast surface display libraries of Spike (S) Ectodomain...Taq polymerase and MMLV reverse transcriptase expression plasmids from the Drew Endy and Philippa Marrack...2020.04.13.039941 (Link opens in a new window) BST-LF expression plasmid from Andrew Ellington's lab. Find plasmid...

  15. CRISPR Plasmids - Parasites

    Type
    Collection
    ...CRISPR expression plasmids that have been designed for use in parasites.... Mammalian Bacteria Drosophila Plant C. elegans Yeast Zebrafish Xenopus CRISPR Resources CRISPR Guide ...

  16. CRISPR Plasmids - gRNAs

    Type
    Collection
    ...gRNA expression vectors available from Addgene.... Mammalian Bacteria Drosophila Plant C. elegans Yeast Zebrafish Xenopus CRISPR Resources CRISPR Guide ...

  17. CRISPR Plasmids - CRISPR Transposases (CAST)

    Type
    Collection
    ... Mammalian Bacteria Drosophila Plant C. elegans Yeast Zebrafish Xenopus CRISPR Resources CRISPR Guide ...Transposase plasmids. Plasmids are available for expression in mammalian systems and bacteria. Mammalian ...

  18. CRISPR References and Information

    Type
    Collection
    ... sgRNA dual expression: pAC2 , pAC152 , pAC153 , pAC154 ; pmax dCas9 activator expression: pAC91 , pAC92... support for bacteria ( E. coli, B. subtilis ), yeast ( S. cerevisiae ), worm ( C. elegans ), fruit fly...genomes are human (hg19 & hg38), mouse (mm10), and yeast (strain w303). Developed by Venetia Pliatsika and...design and cloning All-in-one plasmid containing expression cassette for NmCas9 and BsmBI site for cloning...pSIR-neo) , GFP (pSIR-GFP) , DsRed (pSIR-DsRed-Express2) , human CD2 (pSIR-hCD2) PDF, 108 KB Fujii iChIP...

  19. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ...sensor for Yeast (AT1.03 derived) Dynamic analysis of cytosolic glucose and ATP levels in yeast using optical...Osmolarity SED1 osmolarity biosensor, for expression in E. coli , yeast, or plants Intrinsically disordered ...Kim , GENIE Project Calcium Bicistronic vector expressing GCaMP6 and mRuby2 for imaging neural activity... Huebener , Tobias Rose Calcium Soma-targeted expression of jGCaMP8f, GCaMP7f, or GCaMP6f Precision Calcium...6. Edward Boyden Calcium Intersectional viral expression of GCaMP6m/s in cells depdent on Cre, Flp, or...Karl Deisseroth , INTRSECT 2.0 Project Calcium Expression of GCaMP6f in PV cortical interneurons under ... Aug 4;11(1):3881. Adam Cohen Calcium GCaMP6f expression in forebrain GABA-ergic interneurons under the...

  20. CRISPR Plasmids - Prime Edit

    Type
    Collection
    ...Cas9. ID Plasmid Expression System Promoter Guide RNA Type Cloning Enzyme Co-expressed Cas9 Selection PI... Mammalian Bacteria Drosophila Plant C. elegans Yeast Zebrafish Xenopus CRISPR Resources CRISPR Guide ... editing plasmids. Plasmids are available for expression in mammalian systems, bacteria, plants, and Drosophila...the search bar to find a gRNA vector based on expression system, promoter, the type of gRNA (e.g., pegRNA...
Showing: 101 - 120 of 130 results