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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 1,105 results for: Ide

Showing: 121 - 140 of 1105 results

  1. CRISPR Plasmids - Tagging

    Type
    Collection
    ...Yeast Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog...you will need to design and clone in a gRNA(s) to guide the Cas9 protein to your target sequence, as well...plasmid. The Mendenhall and Myers labs have also provided their protocol for homology arm cloning: Mendenhall...single plasmid targeting vector that encodes both a guide RNA transcript and a repair template for an individual...epitope tags. The plasmids in the following articles provide PCR templates for amplification of the tag (eg ...Nucleic Acids Res. 2014 The Förstemann lab has provided detailed protocols for N- or C-terminal tagging...

  2. Antibodies

    Type
    Collection
    ...control methods. Learn More Guide New to using antibodies? In our Antibody Guide, we define key terms, summarize...experts in the field to develop new antibodies and provide experimental data for recommended applications....Interested in our production process? At Addgene, we provide transparency in our production process ...techniques, and explain common applications. View Guide Need Some Help? Browse some of our other resources...

  3. Antibody Production

    Type
    Collection
    ...phosphate-buffered saline containing 1 mM sodium azide. This buffer is not compatible for use in live cells...sample is separated via a denaturing SDS-PAGE gel alongside serial dilutions of a standard antibody of known... undergoes testing, either in-house or through outside labs, to confirm recognition of the expected target...parental plasmid sequence to confirm the antibody’s identity. For more details on protocols see the Addgene...at room temperature. However, Addgene determines ideal shipping conditions for each order based on the ...

  4. CRISPR Plasmids and Resources

    Type
    Collection
    ...activator peptide activates gene expression. Interfere dCas9 alone, or fused to a repressor peptide, inhibits...parasites, and Xenopus. Addgene CRISPR Resources CRISPR Guide : Essential background information on CRISPR and... CRISPR libraries are a powerful tool for genome-wide screening. Purify dCas9 fused to an epitope tag ...

  5. p53 Pathway

    Type
    Collection
    ...subunits, individual links to each gene page are provided below. Color is used for clarity and does not ...subunits, individual links to each gene page are provided below. Symbol Name 14-3-3-σ Stratifin Apaf-1 Apoptotic...apoptosis inducing protein 1 p53R2 p53 inducible, ribonucleotide reductase M2 B (RRM2B) PAG608 Zinc finger, ... PIDD p53-induced death domain protein 1 PIGs Etoposide induced 2.4 PIRH-2 Ring finger and CHY zinc finger...

  6. Viral Production

    Type
    Collection
    ...Sequencing results are analyzed to confirm the identity and integrity of the viral genome and the absence...Samples of viral preparations are subjected to polyacrylamide gel electrophoresis (PAGE) followed by silver...negative staining. Empty vector particles can be identified after negative staining and appear darker than...to the cleared viral preparation pool. Proviral Identity Confirmation and Sterility All of our lentiviral...

  7. Addgene Packaged on Request: Scope of Service

    Type
    Collection
    ...the time of shipment. If any discrepancies are identified during this analysis, you will be notified by... Addgene performs QC measures which verify the identity of the viral vector, but not the function. Upon... years of the order being shipped, Addgene can provide the QC validation data performed on the material...

  8. Plasmids for Stem Cell Research

    Type
    Collection
    ... to decide which method to use. Addgene's blog post Delivery Methods for Generating iPSCs provides an ... also have the unique potential to continuously divide while maintaining an undifferentiated state. Stem...

  9. mTOR Pathway

    Type
    Collection
    ...subunits, individual links to each gene page are provided below. Color is used for clarity and does not ...subunits, individual links to each gene page are provided below. mTORC1 Complex Symbol Name 4E-BP Eukaryotic...

  10. Zinc Finger Consortium Reagents

    Type
    Collection
    ...additional pages describing these reagents are provided below: Zinc Finger Genome Engineering Reagents...

  11. NETRF

    Type
    Collection
    ...Carcinoid Tumors Bradley Bernstein Broad Institute Identifying Altered Epigenetic States and Drivers in Intestinal...

  12. MAPK Plasmids

    Type
    Collection
    ...also like... Human Kinase ORFs Genome Engineering Guide Empty-Backbone Resource Mitogen-activated protein...

  13. AAV Viral Preps

    Type
    Collection
    ...transduce many mammalian cell types. AAV is used in a wide range of experiments, both in vitro and in vivo....

  14. Plasmid Collections

    Type
    Collection
    ...together based on type or application. Browse our guides to find the plasmids and science information you...

  15. Synthetic Biology - Overview

    Type
    Collection
    ... community. To deposit, view our SynBio Deposit Guide or start our online deposit process . Browse SynBio...

  16. Transferable Skills Guide: Identifying Your Transferable Skills

    Type
    Blog Post
    ...more attractive candidate for that ideal job. How can you identify your transferable skills? PLoS One ...collaboration and fail to identify it as a personal strength until it is identified by another person. Similar...when a new venture presents itself even outside of work, consider challenging yourself, take the opportunity...All while still paying bills and having a life outside my job. The same can be said about teamwork, communication...transferable skills so important? It is important to identify which transferable skills you possess because ... and making connections, avoid falling into an identity trap where you assume your technical skills define...wants to hear a specific time that you thought outside the box to solve a problem. When they ask about...

  17. When Fidelity Matters: A frank discussion about ligase fidelity

    Type
    Blog Post
    ...high fidelity ligase—and when is an alternative ligase a better choice? And what is ligase fidelity anyway...are "higher fidelity") better than T4 DNA Ligase (21, 25, 26). Despite this higher fidelity, Taq DNA Ligase...annealed complementary regions. Why use a high fidelity ligase? Numerous applications have been developed...developed to take advantage of the high fidelity of Taq and other thermostable DNA ligases. These applications...applications detect specific nucleotide sequences with high specificity and quantitative accuracy and even include... include profiling single nucleotide polymorphisms (SNPs) (5,12,13). For example, in the Ligase Detection...PCR amplified fragment) and treated with a high fidelity thermostable DNA ligase. If the target sequence...

  19. Unique and persistent IDs for improved reproducibility: Addgene now supports RRIDs and compact identifiers for all plasmids

    Type
    Blog Post
    ... contain the Addgene ID # and the information necessary to indicate that this ID is managed by Addgene...within the Addgene catalog. These IDs allow you to unmistakably identify a plasmid available through Addgene...VQR-Blast, which has the Addgene ID #87155. The resolvable compact identifier format for lenti-Cas9-VQR is...will be assigned an Addgene ID #, RRID, and resolvable compact identifier. Addgene works hard to ensure...including plasmids. All plasmids at Addgene have unique ID numbers associated with them, which serve as unique...about each plasmid item. Given a specific Addgene ID # listed in a publication, you can quickly pull up...the “Articles Citing this Plasmid” section. Having IDs linked to these metadata and publications help to...

  20. CRISPR Guide Update

    Type
    Blog Post
    ...is proud to present our updated CRISPR Guide! The CRISPR Guide helps scientists understand how to use...incredible new CRISPR technologies developed and widely adopted in the past few years, as well as refreshing...refreshing the existing content. Access the CRISPR Guide here! Highlights include a much-anticipated prime... PRIME editing Please check out the updated guide for a great introduction to what CRISPR technologies...
Showing: 121 - 140 of 1105 results