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We narrowed to 319 results for: virus

Showing: 301 - 319 of 319 results

  4. Plan Your Experiment

    Type
    Collection
    ...lentiviral particles (for details about lentivirus, see our Lentivirus Guide ) Stable, tunable expression ...

  6. Guide to Using Pooled Libraries

    Type
    Guide
    ...the plasmids must first be used to make virus. This pooled virus is subsequently used to deliver the plasmids...electroporation and maxiprep). If delivering as virus, make virus; this creates a pooled lentiviral CRISPR library...

  9. Science Guides

    Type
    Guide
    ...types of viruses that are commonly used in research, including: Lentivirus Adeno-associated Virus (AAV) ...) Adenovirus γ-Retrovirus Read More...

  11. CRISPR Plasmids - Parasites

    Type
    Collection
    ...analyses to pinpoint genes linked to parasite virulence, transmission, and development. This collection...

  13. Educational Resources

    Type
    Guide
    ...applications in molecular biology, plasmid cloning, and virus, including: Gel Electrophoresis Bacterial Transformation...

  15. Chemogenetics Guide

    Type
    Guide
    ...mouse models. For viral injection, Adeno-associated virus (AAV) is a widely-used tool for achieving in vivo... especially useful in AAV experiments where the virus can spread around the injection site and potentially...

  16. Optogenetics Guide

    Type
    Guide
    ...localized expression of the opsin. Depending on the virus and promoter system used, there is an incubation...Sabatini BL 2012. Novel recombinant adeno-associated viruses for Cre activated and inactivated transgene expression...

  17. CRISPR Guide

    Type
    Guide
    ... be efficiently packaged into adeno-associated viruses (AAVs) . Other natural Cas orthologs include Hsp1Cas9...editing method in which Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV RT) is fused to the...H840A nickase and M-MLV RT (Moloney Murine Leukemia Virus Reverse Transcriptase) for "search and replace" ...need to generate lentivirus containing the entire library (Figure 8D). This lentivirus will then be used...delivery to cells that are poorly transduced by lentivirus. Non-mammalian CRISPR libraries are also available...as liquid DNA, or in select cases, as pre-made lentivirus . DNA libraries are shipped at a concentration...need to either co-infect with a Cas9-producing lentivirus or use a Cas9-expressing stable cell line. After...

  18. Plan Your Experiment

    Type
    Guide
    ...lentiviral particles (for details about lentivirus, see our Lentivirus Guide ) Stable, tunable expression ...

  19. Promoters

    Type
    Guide
    ...Constitutive Strong mammalian promoter from human cytomegalovirus EF1a Constituitve Strong mammalian promoter...
Showing: 301 - 319 of 319 results