We narrowed to 1,002 results for: Eras

...reporter) pX458 plasmid family. They have made several modifications to the the pX458 plasmid to expand...Cloning Protocols Single guide cloning: Zhang Lab General Cloning Protocol (PDF, 237 KB) Dual guide cloning...

...which are shipped as bacterial stabs at room temperature. Individual plasmids can be ordered from each...following items will be shipped as stabs at room temperature. ID Name 13421 pAC-Kan-alphaGal4 13422 pBAC-lacZ...

...pathways and protein interactions. Plasmids A diverse set of wild-type MDH genes from several species and mutant...

...pairs of zinc finger arrays that can be used to generate ZFNs targeted to a site within that gene....pairs of zinc finger arrays that can be used to generate ZFNs targeted to a site within that gene. To construct...

... 50 × 100 µL aliquots 5.0 mL *AAV2 yields are generally lower, with a minimum titer of 2 × 10 12 GC/mL... Packaged on Request preps is all-inclusive of several important steps: MTA facilitation for the transfer...

...perform genome edits, Cas9 nuclease is used to generate double-stranded breaks ( DSBs or cuts ) or single-stranded...dCAS-VP64_Blast dCAS9 (D10A, N863A) Blasticidin 3rd generation lenti vector encoding dCAS9-VP64 with 2A Blast...

...an indel or inversion mutation [3]. In the CRISPR era, the genomic landscape may be surveyed in unprecedented...present a video reprint of the CRISPR article "Generation of Genomic Deletions in Mammalian Cell Lines ...between deletion size and frequency [3]. There are several applications in which the production of deletions...within the deletion boundaries may be produced to generate clonal allelic series. Single DSBs resolved by...

...colleagues can be more difficult during the pandemic era, with fewer opportunities for spontaneous conversations...creative interaction During Zoom meetings, different tools can be used to promote creative interaction. A great...here, and only general chatter will be allowed with a different host each time that moderates conversations...An example could be making lab meetings more interactive and creating space for discussion at the end ...end of each talk - time for free interaction and brainstorming. This could be achieved by asking each presenter...also make the screen-sharing experience more interactive. The button is on the right of the Share Screen...writing publications/grants too. As described by several works (Wuchty et al., 2007, Vicens et al., 2007...

...methods for C. elegans were released, signaling a new era in C. elegans research. Homology directed repair ...CRISPR in C. elegans even easier - now, one can generate a fluorescent protein fusion, transcriptional ...efficient selection-based CRISPR/Cas9 method to generate knock-ins on any genetic background. Their new...flanking loxP sites and a heat-shock inducible Cre to generate a self-excising cassette (SEC). To make it possible...

...of drug targets in Alzheimer’s disease (AD) by generating validated resources for emerging targets in AD...

...fused to FokI nuclease. When FokI dimerizes, it generates a double-strand break (DSB) at a specific sequence...

...Browse plasmid tools generated by IGVF Consortium investigators to examine how genomic variation affects...

...with diverse roles in processes such as cell proliferation, differentiation, inflammation, metabolism, ...

...Michael J. Fox Foundation Plasmid Resource iPSC Neurodegenerative Disease Initiative (iNDI) Collection Rett ...

...for "Parasite". The content for this page was generated with the help of Scott Lindner . Cut ID Plasmid...

...CRISPR nickase plasmids for the generation of single-strand breaks....

...mammalian systems, Cas13a does not exhibit the collateral RNA degradation seen in bacteria. Figure 1: Overview...

...Alternatively, gRNAs can be fused to protein-interacting RNA aptamers, which recruit specific RNA-binding...

...systems use a type I Cas effector, composed of several separate Cas enzymes that form a complex called...

...Fluorescent Proteins: Subcellular Localization Luciferase Plasmid Collection Viral Vector Packaging Service...