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Showing: 121 - 140 of 997 results

  1. CRISPR Plasmids - Empty gRNA Vectors

    Type
    Collection
    ...Joung Multiplex gRNA Vectors Several systems have been developed to generate multiplex gRNA vectors. These...entries describing enzyme activity, experimental considerations, and more, created and maintained by the Doudna...Dicot Plants Plant PCR-based procedure to rapidly generate multiple sgRNA expression cassettes, which can...

  2. Validated gRNA Sequences

    Type
    Collection
    ...lC.GA4a B. oleracea GTTGAGAGGGGAGCCGGTGA 68256 cut S. pyogenes 26616834 Patron lC.GA4a B. oleracea GTTTTCACTTGCGGCCGGAG...AGACGACCCTGTCATCCGCA 74188 cut S. pyogenes 26627737 Moffat Luciferase ACAACTTTACCGACCGCGCC 74190 cut S. pyogenes 26627737...

  3. Michael J Fox Foundation (MJFF) Plasmid Collection

    Type
    Collection
    ...research. This collection is part of Addgene's Neurodegeneration Research Collection , which includes other...Research Tools Labs affiliated with MJFF have also generated a number of Shared Research Tools (Link opens ...

  4. Michael Davidson Fluorescent Protein Collection

    Type
    Collection
    ...blog post for more information. Empty Backbones mEmeralds Reds Blues Cyans Oranges Greens Yellows Photoactivatables... Empty Backbones Plasmid Description Industry mEmeralds Plasmid Description Industry Reds Plasmid Description...

  5. Viral Vectors 101: Types of viruses

    Type
    Blog Post
    ...Potential Applications for Gene and Cell-Based Therapies in the New Era of Personalized Medicine. Genes Dis. 2017...is high, it’s generally not as long lasting as AAV (up to several weeks vs. AAV’s several years). But these... To add insult to injury, retroviruses generally have moderate to high immunogenicity, meaning they induce...interchangeable between the two systems, other, more general, viral components such as a heterologous envelope...AAV has many excellent features for human gene therapy and is currently being used in clinical settings...adenovirus have been identified so far! Adenovirus generally has high immunogenicity, but some strains have... fact: All viruses discussed in this blog are generally broadly infective, with one exception. Blood cells...

  6. Ginkgo Bioworks COVID-19 Collection

    Type
    Collection
    ...window) Researchers developing novel diagnostics, therapeutics, and vaccines against COVID-19 all rely on DNA...researchers to express SARS-CoV-2 viral proteins in several expression systems. Addgene has partnered with ...

  7. CRISPR Plasmids - Single-Strand Break (Nick)

    Type
    Collection
    ...CRISPR nickase plasmids for the generation of single-strand breaks....entries describing enzyme activity, experimental considerations, and more, created and maintained by the Doudna...

  8. Cancer Research Plasmids and Resources

    Type
    Collection
    ...and Libraries for Cancer Research Broad Target Accelerator Plasmids: Collection of vectors from the Boehm...mutant alleles described in the initial Target Accelerator publications, focused on somatic variants found...

  9. CRISPR Plasmids - RNA Targeting

    Type
    Collection
    ...mammalian systems, Cas13a does not exhibit the collateral RNA degradation seen in bacteria. Want more information...entries describing enzyme activity, experimental considerations, and more, created and maintained by the Doudna...

  10. Institute for Protein Innovation

    Type
    Collection
    ...sorting process. This combination allows IPI to generate antibodies to highly conserved proteins and other... with a mission to advance protein science to accelerate research and improve human health. As part of...

  11. Plasmid Collections

    Type
    Collection
    ...Zinc Fingers Luminescence Fluorescent Proteins Luciferase Optogenetics Chemogenetics Viral Plasmids Lentivirus... Neuroendocrine Tumor Research Foundation Neurodegeneration Research Collection Rett Syndrome Plasmid ...

  12. CRISPR Plasmids - Cascade-Cas3

    Type
    Collection
    ...deletions generated by Cas9, large deletions created using Cas3 have fewer off-target alterations and require...

  13. Brzezinski Lab CRISPR Collection

    Type
    Collection
    ...reporter) pX458 plasmid family. They have made several modifications to the the pX458 plasmid to expand...Cloning Protocols Single guide cloning: Zhang Lab General Cloning Protocol (PDF, 237 KB) Dual guide cloning...

  14. DNA Service - Cloning Grade DNA

    Type
    Collection
    ...Standard Quality Control Plasmid verified by next generation sequencing Plasmids Available as Cloning Grade...the plasmid sequence has been validated by next generation sequencing. The DNA concentration and 260/280...

  15. Zinc Finger Consortium: OPEN Reagents

    Type
    Collection
    ...which are shipped as bacterial stabs at room temperature. Individual plasmids can be ordered from each...following items will be shipped as stabs at room temperature. ID Name 13421 pAC-Kan-alphaGal4 13422 pBAC-lacZ...

  16. CRISPR Plasmids - Base Edit

    Type
    Collection
    ...and adenine base editors (ABEs) — can be used to generate single base pair edits without double stranded...entries describing enzyme activity, experimental considerations, and more, created and maintained by the Doudna...

  18. Synthetic Biology - Overview

    Type
    Collection
    ... Jeff Hasty Dieter Jahn Alfonso Jaramillo Roman Jerala Jay Keasling Taek Soon Lee Joshua Leonard Han Lim...Architecture Open Plant - Plant SynBio resource EraSynBio - The EU/USA SynBio initiative Recent Synthetic...

  19. Zinc Finger Consortium: Zinc Finger Arrays

    Type
    Collection
    ...pairs of zinc finger arrays that can be used to generate ZFNs targeted to a site within that gene....pairs of zinc finger arrays that can be used to generate ZFNs targeted to a site within that gene. To construct...

  20. Qi Lab CRISPR Page

    Type
    Collection
    ...interfering with transcriptional elongation, RNA polymerase binding, or transcription factor binding. The...region. The uniqueness of CRISPRi, as compared to several recently published works on applying the wild-type...
Showing: 121 - 140 of 997 results