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We narrowed to 965 results for: REP

Showing: 121 - 140 of 965 results

  1. Caltech Systemic Capsids

    Type
    Collection
    ..., Addgene is distributing ready-to-use viral preparations in engineered systemic serotypes from the Viviana...and full sequencing of the final viral vector preparation. Systemic Delivery AAV Capsid Choice Need help...Addgene’s Data Hub and the Caltech CLOVER Center’s repository of published capsid use (Link opens in a new ...: Systemic Capsids PHP.eB These viral vector preparations were produced with the pUCmini-iCAP-PHP.eB plasmid...tropism exhibited by the PHP.eB serotype has been reported to occur by using the cellular receptor LY6A (...expression iCre AIBS , Ting PHP.S These viral vector preparations were produced with the pUCmini-iCAP-PHP.S plasmid...tdTomato Control Boyden PHP.V1 These viral vector preparations were produced with the pUCmini-iCAP-PHP.V1 plasmid...

  2. Plan Your Experiment

    Type
    Collection
    ...genome editing. Scientific Reports , 5 , 11315. https://doi.org/10.1038/srep11315 PMID: 26066060 Zukher, ...experimentally verified Cut Nick Homology Directed Repair (HDR) Generate specific genomic edits, like point...fusions with proteins that promote HDR Requires a repair template Lower editing efficiency compared to NHEJ...pegRNA optimization can be challenging Prime Edit Repress or Interfere (CRISPRi) Reduce gene expression without...sequence dCas9 or dCas9 fusion with transcriptional repressor (such as dCas9-KRAB) dCas9-KRAB can be more effective...requires donor DNA to serve as template for the repair. Once you settle on your gRNA, you can design a...target in your samples to ensure there are no discrepancies with the reference sequence you used for gRNA...

  3. Brain Initiative Collection

    Type
    Collection
    ...Plasmid Description PI Viral Preps Addgene distributes ready-to-use viral preps made from plasmids in our... BRAIN Initiative collection of plasmids, viral preps, and antibodies here. Collections...the research community. Browse plasmids , viral preps , and antibodies created with support from the BRAIN...use. Learn more about our viral service ID Viral Prep Description PI 65417-AAV8 pAAV-hSyn-dF-HA-KORD-IRES-mCitrine...plasmid encoding for Archon1 fluorescent voltage reporter, Cre-dependent expression Edward Boyden 108912...Expresses the genetically-encoded fluorescent norepinephrine(NE) sensor GRAB_NE1m in neurons Yulong Li 123308...Expresses the genetically-encoded fluorescent norepinephrine(NE) sensor GRAB_NE1m in neurons Yulong Li 123309...

  4. Genetic Code Expansion

    Type
    Collection
    ...tyrS gene knocked out, tyrTV gene replaced by tolC, and tyrU gene replaced by GentR). Expresses archaeal ...tyrTV gene replaced by tRNA variant that suppress the TAG nonsense codon, and tyrU gene replaced by tRNA ...tyrS gene knocked out, tyrTV gene replaced by tolC, and tyrU gene replaced by GentR) and release factor 1...biopolymers. Tips for Success Before beginning to reprogram the genetic code, there are several things to ... are true. Always express first with a control reporter gene: GFP for E. coli or mCherry-GFP for mammalian...RF1 function removed, with Ubiquitin-UAG-sfGFP reporter George Church 98565 C321.ΔClpS.Ub-UAG-sfGFP all...removed, ClpS inactivated, with Ubiquitin-UAG-sfGFP reporter George Church 174513 Syn61 No TCG, TCA, or TAG...

  5. TALEN Guide

    Type
    Collection
    ...amino acid repeats. These repeats only differ from each other by two amino acids, their repeat-variable ... of 18 TAL effector repeats. The kit can be used to make arrays from 12-31 repeats. Perhaps one of the... to this DNA targeting ideal. Figure 1: (A) Representation of a zinc finger (ZF) nuclease. Zinc fingers...if not slightly lower. Figure 2: Simplified representation of the Voytas/Bogdanove Golden Gate TALEN kit... would expect that assembling relatively small, repeat-laden DNA regions into a single construct would...from Addgene , custom arrays consisting of 12-31 repeats can be assembled and inserted into a variety of...

  6. SARS-CoV-2 Pseudotyped Virus

    Type
    Collection
    ...Envelope and Packaging Plasmids Reporter Plasmids A few examples of reporter plasmids that can be used for...-CoV-2 spike plasmids, packaging plasmids, and reporter plasmids for viral pseudotyping with SARS-CoV-...vesicular stomatitis virus G) are packaged as replication-defective virus particles with the SARS-CoV-2...44965 NL4-3 mCherry Luciferase Lentiviral dual reporter vector expressing mCherry and firefly luciferase...Greene 105621 Lenti-luciferase-P2A-Neo Lentiviral reporter vector expressing firefly luciferase with neomycin... pHAGE-CMV-Luc2-IRES-ZsGreen-W Lentiviral dual reporter vector expressing firefly luciferase and ZsGreen...vector expressing GFP and luciferase for use as a reporter in pseudovirus production and infection. Dapeng...

  7. Promega Plasmid Collection

    Type
    Collection
    ... and more. Luciferase Reporters Luciferase (Link opens in a new window) reporter assays use light produced...Find luciferase reporter vectors for sensitive gene expression analysis and fusion vectors containing... workflows. The collection contains luciferase reporter vectors for sensitive gene expression analysis...collection. For example, search by response element, reporter, protein, or tag, such as "NFAT", "luciferase"... a new window) is a structural complementation reporter system composed of a Large BiT (LgBiT; 18 kDa)...Addgene Find additional information about luciferase reporter assays (Link opens in a new window) from Promega...blogs for technical tips and tools for genetic reporter assays (Link opens in a new window) and NanoBRET...

  8. Brzezinski Lab CRISPR Collection

    Type
    Collection
    ...modifications: replacement of the Cbh promoter with EF1alpha addition of an mCherry reporter variant nuclear...utilizes a CRISPR/Cas9 based approach to knockout, repress, and activate gene targets. To accomplish this ...advantage of the Zhang lab all-in-one (guide/Cas9/reporter) pX458 plasmid family. They have made several ...nuclear reporters via an H2B fusion shuttle plasmid for shuttling U6-guide cassettes to make dual guide ...fusion for gene and non-coding enhancer sequence repression dCas9-VPR fusion for gene activation CRISPR/Cas9...175573 and 175574 express dCas9-KRAB-MeCP2 for repression. Plasmids 175571 and 175572 express dCas9-VPR...

  9. CRISPR Plasmids - Double-Strand Break (Cut)

    Type
    Collection
    .... When a repair template is present, the cell may repair a DSB using homology-directed repair (HDR) instead...gRNA-defined target sequence. DSBs are preferentially repaired in the cell by non-homologous end joining (NHEJ...introduce specific genomic changes, researchers use DNA repair templates with homology to the DNA flanking the... our CRISPR reference page . CRISPR Guide Viral Preps CRISPR Protocols gRNA Design Tools CRISPR Blogs ...

  10. Antibody Production

    Type
    Collection
    ...to generate high quality preparations. Following production, all antibody preps are titered and undergo...Addgene does not recommend repeated freeze thaw of antibodies and suggests preparing single-use aliquots before...Innovation Antibody Collection webpage. Antibody preparations developed at IPI may differ from Addgene antibodies...Sanger Sequencing Purified recombinant antibody preps contain low levels of co-purified plasmid from transfection...

  11. Lentivirus Plasmids

    Type
    Collection
    ...drives expression of RFP as a reporter. See plasmid 17618 for GFP reporter. Linzhao Cheng 17452 pLenti ...Expresses shRNA under mouse U6 promoter. CMV-EGFP reporter cassette is included to monitor expression. Luk...Expresses shRNA under mouse U6 promoter. CMV-EGFP reporter cassette is included to monitor expression. Stephan...86849 pBOB-EF1-FastFUCCI-Puro 3rd Encodes FUCCI reporters for cell cycle analysis. Puromycin selection. ...Addgene blog: Tips for Titering Your Lentiviral Preps Content last reviewed: 23 October 2025 Do you have...

  12. CRISPR Plasmids - Single-Strand Break (Nick)

    Type
    Collection
    ...be used with a repair template to introduce specific edits via homology-directed repair (HDR). Figure ...nicks create a double-strand break (DSB) that is repaired using error-prone non-homologous end joining (...Figure 1: Overview of Cas9 nickase cleavage and repair. Browse, sort, or search the tables below for CRISPR... our CRISPR reference page . CRISPR Guide Viral Preps CRISPR Protocols gRNA Design Tools CRISPR Blogs ...

  13. CRISPR Plasmids for Genomic Visualization

    Type
    Collection
    ...has been used for dynamic tracking of repetitive and non-repetitive genomic loci, as well as chromosome ...given region. For example, chromosome-specific repetitive loci can be efficiently visualized in living ... targeted sequences in close proximity. A non-repetitive genomic locus can also be labeled by co-delivering... our CRISPR reference page . CRISPR Guide Viral Preps CRISPR Protocols gRNA Design Tools CRISPR Blogs ...

  14. TALEN Engineering

    Type
    Collection
    ...developed three platforms for engineering TAL effector repeat arrays of any desired length: the REAL (Restriction... three of these platforms assemble TAL effector repeat arrays on a single architectural framework and ...methods are identical in sequence. TAL effector repeat arrays produced on this framework has yielded TALENs...Lab REAL Assembly TALEN Kit Individual REAL TALE Repeat Plasmids TALEN Expression Vectors for REAL, REAL-Fast...for Endogenous Human Genes TALENs for the EGFP Reporter Gene Additional Plasmids compatible with the REAL...

  15. Addgene Packaged on Request: Scope of Service

    Type
    Collection
    ...limited use label licenses (LULLs). Titer For AAV preps produced through our Packaged on Request service... we may expect lower titers for a particular AAV prep, mostly likely due to one of the following reasons...moving forward with a helpful solution. You must reply to that email within 10 business days in order to...been completed at the time of shipment. If any discrepancies are identified during this analysis, you will...moving forward with a helpful solution. You must reply to this email within 10 business days in order to...

  16. AAV for Neuronal Tracing

    Type
    Collection
    ...which is not required for the transcription or replication of the genome within infected cells, but is required... cells that lack the G protein, the virus can replicate but the newly formed viruses are unable to infect...starter cell via the EnvA receptor. RABV is able to replicate in the starter cell using the G-protein, which...eligible AAV plasmids and we’ll make the viral vector prep you need. Or fill out our form to let us know what...what you would like to see available in viral prep format. Please note this does not guarantee viral vector...

  17. Zebrafish Plasmid Collection

    Type
    Collection
    ...editing, empty backbones, fluorescent proteins, reporters, biosensors, and Cre expression plasmids. Large...studying diseases, are listed below: Lipid droplet reporters - Richard White Lab. An imaging platform to study...using red light. LipoGlo - Steven Farber Lab. A reporter system that allows direct assessment of lipoprotein...International Resource Center (ZIRC) - A US-based repository for zebrafish strains and materials and information...European Zebrafish Resource Center (EZRC) - A repository at the Karlsruhe Institute of Technology (KIT...

  18. Immunology Research Plasmids and Resources

    Type
    Collection
    ...leucine-rich repeat-containing G protein-coupled receptor 4 GPR48 LGR5 leucine-rich repeat-containing G... to the formation of a high avidity BCR through repeat cycles of expansion. Chemokines Chemokines, or ... to the formation of a high avidity BCR through repeat cycles of expansion. Symbol Name Synonyms AKT1 ...MGC102867 SEMA5A sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain ... semF SEMA5B sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain ...LGR1, MGC141667, MGC141668, ODG1 GAL galanin prepropeptide GALN, GLNN, GMAP, MGC40167 GALP galanin-like...GHRFR, GHRHRpsv, GRFR GHRL ghrelin/obestatin prepropeptide MTLRP, obestatin GHSR growth hormone secretagogue...

  19. CRISPR Plasmids - Epigenetics

    Type
    Collection
    ...demethylation DNMT3A — repression through cytosine methylation MQ1 — repression through cytosine methylation...methylation with improved kinetics LSD1 — repression through targeted removal of H3K4 and H3K9 methylation... our CRISPR reference page . CRISPR Guide Viral Preps CRISPR Protocols gRNA Design Tools CRISPR Blogs ...

  20. Synthetic Biology - Overview

    Type
    Collection
    ... modification, including shuttle, integration, reporter, and tagging vectors. Metabolism Browse plasmids...regulatory elements, including promoters, terminators, repressors, activators, and more. Also contains pre-assembled...BioBrick Box Mukhopadhyay Lab Efflux Pumps Voigt Lab Repressors , Terminators , Light Signaling , Sigma Factors...Foundation Registry of Standard Biological Parts - Repository of community-created BioBricks JBEI - Joint Bio-Energy...
Showing: 121 - 140 of 965 results