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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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In Category

We narrowed to 1,036 results for: STI

Showing: 121 - 140 of 1036 results

  1. Adenovirus Plasmids

    Type
    Collection
    ...Adenoviral plasmid construction Frequently asked questions about adenovirus Glossary of commonly used adenovirus...adenovirus genomes Bunz Return to Top Do you have suggestions for other plasmids that should be added to this...

  2. Retrovirus Plasmids

    Type
    Collection
    ...γ-Retroviral packaging systems Frequently asked questions (FAQ) about retrovirus Popular γ-Retrovirus Envelope...mammalian cells Wong Return to Top Do you have suggestions for other plasmids that should be added to this...

  3. Lentivirus ddPCR Titration

    Type
    Protocol
    ...ensure that you are in compliance with your institution’s biosafety regulations. Last Update: July 7, ... µg/mL. Mix well and incubate 72 h. Benzonase Digestion and Cell Harvest Gently aspirate media from the... When analyzing data there should be a clear distinction between negative droplets (black) and positive...

  4. CRISPR Plasmids - Prime Edit

    Type
    Collection
    ...U6 pegRNA + nicking sgRNA BbsI for sgRNAs; BbsI + PstI for pegRNAs No Xiao Wang 170132 pOsU3 Plant OsU3...Esp31 for PBS-RT No BFP Ervin Welker Do you have suggestions for other plasmids that should be added to this...

  5. Plant Plasmids and Resources

    Type
    Collection
    ... simplifies gene silencing and genome editing logistics - Jose-Antonio Daros. Return to top Empty Backbones... RNA-seq analysis. Return to top Do you have suggestions for other plasmids that should be added to this...

  6. CRISPR References and Information

    Type
    Collection
    ...Frederick National Laboratory at the National Cancer Institute. Cas-OFFinder (Link opens in a new window) Identifies... , pAC1 , pAC147 , pAC148 , pAC149 ; Gateway destination: pAC90 PDF, 1.1 MB Katic Nematode: Cas9 and gRNA...

  7. Protocol - How to Purify DNA from an Agarose Gel

    Type
    Protocol
    ...for your eyes!), remove it from any gel tray as plastic will block much of the UV and with a clean, sterile... gel on a glass plate if available. Unlike the plastic tray, this will not significantly reduce the UV...

  8. Protocol - How to Perform Sequence Analysis

    Type
    Protocol
    ...Plasmid You may also like... How to Design a Primer Diagnostic Restriction Digest Introduction Sequence verification...the peaks in the area and make sure they are justifiable peaks. For instance, in the trace file below...

  9. Ligation Independent Cloning

    Type
    Protocol
    ...recognition sequence. This can create multiple distinct overhangs with a single enzyme, and remove the...now look like this: Note: Elute your purified digestion and PCR products with sterile water (instead of...

  10. Centrifugation

    Type
    Protocol
    ...been placed in the centrifuge before you and may still be on the equipment. Before using the centrifuge...centrifuge. Immediately stop the centrifuge and investigate the issue. When you are finished spinning your...

  11. Lab Safety for Biosafety Levels One and Two

    Type
    Protocol
    ...BSL-2). The purpose of the four levels is to distinguish between different types of hazards and to provide...with additional precautions to prevent injuries, ingestion, and exposures to hazardous materials. Video Watch...

  12. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...Parkinson's Christien Merrifield 27700 Hip1R-tDimerRFP-N1 Hip1R RFP CMV Parkinson's Christien Merrifield...ClinVar) and is meant to be representative but not exhaustive. If you do not see a particular gene of interest...114410 AICSDP-64: FUS-mEGFP FUS GFP ALS Allen Institute for Cell Science 114601 pTWIN1-His6-Ssp-Htt18-...Ward 178171 SGP11_Halo_N_allele SGP11 Halo ALS, Spastic paraplegia Michael Ward 178172 TBK1_Halo_C_allele...Browse all Neurodegeneration Plasmids Do you have suggestions for other plasmids that should be added to this...

  13. AAV Titration by qPCR Using SYBR Green Technology

    Type
    Protocol
    ...titers were similar to those reported by these institutions. This protocol is for a 96-well plate with 20...reaction volume. Last Update: February 13, 2019 Estimate of time required: ~3 h Workflow Timeline Plate...

  15. AAV ddPCR Titration

    Type
    Protocol
    ...ensure that you are in compliance with your institution’s biosafety regulations. Equipment Class II, Type... When analyzing data there should be a clear distinction between negative droplets (black) and positive...

  16. Kit Free RNA Extraction

    Type
    Protocol
    ...entire tube is dried but while the white pellet is still visible. Resuspend RNA pellet in RNase-free water...

  17. Synthetic Biology - Algal

    Type
    Collection
    ...Insert Vector Type PI Publication Do you have suggestions for other plasmids that should be added to this...

  18. Synthetic Biology - Bacterial

    Type
    Collection
    ...Insert Vector Type PI Publication Do you have suggestions for other plasmids that should be added to this...

  19. Synthetic Biology - Fungal

    Type
    Collection
    ...Insert Vector Type PI Publication Do you have suggestions for other plasmids that should be added to this...

  20. Synthetic Biology - Mammalian

    Type
    Collection
    ...Insert Vector Type PI Publication Do you have suggestions for other plasmids that should be added to this...
Showing: 121 - 140 of 1036 results