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Showing: 121 - 140 of 435 results
  1. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    ...substrate, luciferin, which releases energy as a photon. The photon energy is transferred to the acceptor fluorophore...pcDNA3.1+-pHlash, that use Bioluminescence Resonance Energy Transfer (BRET) were developed by the Johnson Lab...fusion protein (referred to as dCas9-VPR) had a synergistic effect on target gene activation. The novel dCas9...rely on the principle of bioluminescence resonance energy transfer (BRET), whereby photons emitted by a Renilla...
  2. Antibodies 101: Flow Cytometry

    Type
    Blog Post
    ...permeabilization with detergents such as triton-x, saponin, or tween. Take care when using strong detergents like triton-x...resources References: Bonner WA, Hulett HR, Sweet RG, Herzenberg LA (1972) Fluorescence Activated Cell Sorting...
  3. Antibody Plasmid Collection

    Type
    Collection
    ...monoclonal antibodies are produced in-house and undergo application-specific validation and quality control...
  4. Distribution to Industry

    Type
    Collection
    ...monoclonal antibodies are produced in-house and undergo application-specific validation and quality control...
  5. Microbiology Resources

    Type
    Collection
    ...Cyanobacteria Cyanobacteria Plasmids for Fungi Species Aspergillus sp. Cryptococcus sp. Plasmids for Prions Prions...
  6. Worm Expression Resources

    Type
    Collection
    ...promoter. cGAL and Split cGAL plasmids - Paul Sternberg Lab. Plasmids for the temperature-robust GAL4-...
  7. TALEN Guide

    Type
    Collection
    ...Jens Boch at the Martin-Luther-University Halle-Wittenberg and Adam Bogdanove at Iowa State University published...
  8. Zebrafish Plasmid Collection

    Type
    Collection
    ...ortholog in zebrafish, this species has more recently emerged as a model system for human disease and translational...
  9. Fueled by Coffee at #SfN14

    Type
    Blog Post
    ...accounts for many, many lattes and espressos. The energy boost is well needed to keep up with all the awesome...great opportunity – so if you’re running out of energy, then it’s time for a coffee run! ...
  10. Save Time and Money by Making Your Own Competent Cells

    Type
    Blog Post
    ...few examples are provided here for reference: Untergasser Lab Preparation of chemically competent cells... subsequent transformation protocols below: Untergasser Lab Transformation of chemically competent cells...
  11. Future of Research Conference - Remarkable Opening Session

    Type
    Blog Post
    ...effort was led by two amazing postdocs, Kristin Krukenberg and Jessica Polka from Harvard Medical School...Bourne charged the audience to use their useful energy and to choose institutions with allies engaged ...
  12. London Calling - Addgene’s New Europe Office in London

    Type
    Blog Post
    ...spondylarthritis, using Bioluminescence Resonance Energy Transfer (BRET) technique, and plasmids to study...and worked around the world, carrying out her undergraduate work at the University of London and the University...
  13. Antibodies 101: Fc Effector Functions

    Type
    Blog Post
    ...being opened in the pathogen’s cell membrane (Dunkelberger & Song, 2010). As cells cannot survive when ...):443-6. doi: 10.1038/74704. PMID: 10742152. Dunkelberger, J., Song, WC. Complement and its role in innate...
  14. Sensing Neuronal Dopamine

    Type
    Blog Post
    ...understand about Dopamine? DA is produced by specific dopaminergic neurons in a brain region called the substantia... Report endogenous DA release from a single dopaminergic fiber in mouse brain slices Demonstrate DA detection...
Showing: 121 - 140 of 435 results