We narrowed to 435 results for: erg
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TypeBlog Post...substrate, luciferin, which releases energy as a photon. The photon energy is transferred to the acceptor fluorophore...pcDNA3.1+-pHlash, that use Bioluminescence Resonance Energy Transfer (BRET) were developed by the Johnson Lab...fusion protein (referred to as dCas9-VPR) had a synergistic effect on target gene activation. The novel dCas9...rely on the principle of bioluminescence resonance energy transfer (BRET), whereby photons emitted by a Renilla...
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New England Biolabs Cell-Imaging Plasmid Collection
TypeCollection...8896-7. PMID:15264811 Stachelhaus T, Mootz HD, Bergendahl V, Marahiel MA.1998. Peptide bond formation in... -
Antibodies 101: Flow Cytometry
TypeBlog Post...permeabilization with detergents such as triton-x, saponin, or tween. Take care when using strong detergents like triton-x...resources References: Bonner WA, Hulett HR, Sweet RG, Herzenberg LA (1972) Fluorescence Activated Cell Sorting... -
Fluorescent Proteins 101: Monitoring Cell Mobility Using Fluorescent Proteins
TypeBlog Post.... Two low-energy photons (typically from the same laser) cooperate to cause a higher-energy electronic...photoconvertible Keade proteins (protein that undergoes light-induced irreversible photoconversion from... -
Antibody Plasmid Collection
TypeCollection...monoclonal antibodies are produced in-house and undergo application-specific validation and quality control... -
Distribution to Industry
TypeCollection...monoclonal antibodies are produced in-house and undergo application-specific validation and quality control... -
Microbiology Resources
TypeCollection...Cyanobacteria Cyanobacteria Plasmids for Fungi Species Aspergillus sp. Cryptococcus sp. Plasmids for Prions Prions... -
Worm Expression Resources
TypeCollection...promoter. cGAL and Split cGAL plasmids - Paul Sternberg Lab. Plasmids for the temperature-robust GAL4-... -
TALEN Guide
TypeCollection...Jens Boch at the Martin-Luther-University Halle-Wittenberg and Adam Bogdanove at Iowa State University published... -
Zebrafish Plasmid Collection
TypeCollection...ortholog in zebrafish, this species has more recently emerged as a model system for human disease and translational... -
Bringing Sustainable Practices to the Lab: Easy Action Items
TypeBlog Post...aware of our own consumption. Quick ways to reduce energy consumption in the lab Here are a few easy and ...autoclaved together. The autoclave uses a lot of energy, so even one less run a week makes a big difference... -
Fueled by Coffee at #SfN14
TypeBlog Post...accounts for many, many lattes and espressos. The energy boost is well needed to keep up with all the awesome...great opportunity – so if you’re running out of energy, then it’s time for a coffee run! ... -
Save Time and Money by Making Your Own Competent Cells
TypeBlog Post...few examples are provided here for reference: Untergasser Lab Preparation of chemically competent cells... subsequent transformation protocols below: Untergasser Lab Transformation of chemically competent cells... -
IBEX Knowledge-Base: A Data Resource for Multiplex Tissue Imaging
TypeBlog Post...inactivation details, tissue preservation method, and detergents that they used in their experiments. Researchers..., method, tissue preservation, target tissue, detergent, antigen retrieval conditions, and more. Users... -
Future of Research Conference - Remarkable Opening Session
TypeBlog Post...effort was led by two amazing postdocs, Kristin Krukenberg and Jessica Polka from Harvard Medical School...Bourne charged the audience to use their useful energy and to choose institutions with allies engaged ... -
London Calling - Addgene’s New Europe Office in London
TypeBlog Post...spondylarthritis, using Bioluminescence Resonance Energy Transfer (BRET) technique, and plasmids to study...and worked around the world, carrying out her undergraduate work at the University of London and the University... -
Antibodies 101: Fc Effector Functions
TypeBlog Post...being opened in the pathogen’s cell membrane (Dunkelberger & Song, 2010). As cells cannot survive when ...):443-6. doi: 10.1038/74704. PMID: 10742152. Dunkelberger, J., Song, WC. Complement and its role in innate... -
Sensing Neuronal Dopamine
TypeBlog Post...understand about Dopamine? DA is produced by specific dopaminergic neurons in a brain region called the substantia... Report endogenous DA release from a single dopaminergic fiber in mouse brain slices Demonstrate DA detection... -
Why Add Sucrose? Improved Yields for Adeno-associated Virus Preparation
TypeBlog Post...proteoglycans on the surface of the packaging cells (Vandenberghe, 2010). Rather than being released into the ...22915587. PubMed Central PMCID: PMC3476254. 7. Vandenberghe, Luk H., et al. "Efficient serotype-dependent... -
Safe Port in a Storm...How Addgene is Weathering the Pandemic
TypeBlog Post...need, we're incredibly grateful that the Chan Zuckerberg Initiative was able to step in to be our “port...laboratories all around the world." We thank the Chan Zuckerberg Initiative for supporting Addgene by providing...