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TypeCollection...the location and type of MECP2 mutation can be a strong predictor of symptom severity. Broadly, early gene... in a new window) RSRT iPSC lines and isogenic controls , patient-specific Infinity BiologiX - RSRT cell...refer to Addgene's CRISPR Guide for a general introduction to CRISPR technology or the mammalian CRISPR...
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CRISPR Pooled gRNA Libraries
TypeCollection...mind: CRISPR screening experiments require electroporation to amplify the pooled library and the use of...GBC) 85968 Barcode Human Weissman 3rd N/A N/A Retroviral Mouse Genome-wide CRISPR Knockout Library 104861...104861 Knockout Mouse Teichmann N/A (retroviral) 5 90,230 RNA-Binding Protein Pooled CRISPR Knockout Library... -
Fluorescent Protein Guide: Empty Backbones
TypeCollection...pHIV-Zsgreen - Mammalian Expression (this is a bicistronic vector rather than a fusion protein vector) ZsGreen1...- Mammalian Expression pMXs-mRFP1 - Mammalian Retroviral Expression mCherry 587 610 16 4.5 15 min Monomer...proteins Insect Sutherland Lab Plasmids - For multicistronic expression of GFP and/or mCherry, with neomycin... -
Antibodies
TypeCollection...application-specific validation and consistent quality control measures. We work with trusted partners and experts... by sharing our protocols and quality control methods. Learn More Guide New to using antibodies... -
CRISPR Plasmids - Single-Strand Break (Nick)
TypeCollection... CRISPR Blog Posts CRISPR/Cas nickase mutants introduce gRNA-targeted single-strand breaks in DNA instead...mutants can also be used with a repair template to introduce specific edits via homology-directed repair (HDR... -
CRISPR Plasmids - Parasites
TypeCollection... Cut Fully functional CRISPR/Cas enzymes will introduce a double-strand break (DSB) at a specific location...frameshifts, creating loss of function alleles. To introduce specific genomic changes, researchers use ssDNA... -
AAV Molecular Tools
TypeCollection... These AAV encode tetracycline-inducible tools/controls and tetracycline transactivators that can be used...TEVp EF1a-driven, Cre-dependent Cre-dependent, bicistronic expression of designer pro-taCasp3 and TEVp for... -
Adenovirus Plasmids
TypeCollection...100% efficiency. Unlike lentiviruses or other retroviruses , adenoviruses do not integrate into the host...pShuttle-CMV-F2A-T2A-Venus Shuttle For production of viruses with multicistronic expression + Venus tag; See plasmid 62622 for... -
Distribution to Industry
TypeCollection...undergo application-specific validation and quality control by Addgene as well as by our trusted partner labs...Acknowledgement (RSA). This form can be completed electronically by the scientist who will be using the materials... -
Allen Institute for Cell Science Plasmid Collection
TypeCollection...stress granules 114411 TNNI1-mEGFP AICSDP-34 mEGFP Troponin I, slow skeletal type Sarcomeric thin filaments...Nuclear speckles 159744 DMD-mEGFP AICSDP-55 mEGFP Dystrophin Costameres 159745 TFAM-mEGFP AICSDP-72 mEGFP ... -
Brain Armamentarium
TypeCollection...fluorescent protein) in striatal Pvalb+ neurons. Note strong cortical Pvalb and L5 ET neuron expression. Jonathan...3xCore version of DLX enhancer). AAV vector for strong & rapid SYFP2 expression in forebrain GABAergic... -
Ras Pathway
TypeCollection...construction of RAS pathway gene expression vectors for in vitro and in vivo research. The RAS Initiative - at the...colorectal cancer. Zenonos K, Kyprianou K. World J Gastrointest Oncol. 2013 May 15; 5(5): 97–101. doi: 10.4251... -
COVID-19 Resources
TypeCollection...available and more are currently undergoing quality control analysis in our lab and will be made available ... Plasmid Description Industry PI Due to export control regulations, MERS and SARS-CoV materials are available... -
Tags and Other Markers
TypeCollection...undergo application-specific validation and quality control by Addgene as well as by our trusted partner labs... -
Neuroscience
TypeCollection...undergo application-specific validation and quality control by Addgene as well as by our trusted partner labs... -
Synthetic Biology - Metabolism
TypeCollection...Biofuels Natural Product Pathways Efflux Pumps Flux Control Metabolism Plasmids Search the table by keyword... -
Fluorescent Protein Guide: Activity Regulation
TypeCollection...factors Connie Cepko See Plasmids Dronpa Optical control of protein activity Michael Lin See Plasmids Do... -
NETRF
TypeCollection...treatment of neuroendocrine cancers. As such, NETRF strongly encourages its funded scientists to make their... -
TALEN Expression Vectors
TypeCollection...mammalian cell expression and a T7 promoter for in vitro transcription of RNA. TALENs expressed from these... -
Cancer Research Plasmids and Resources
TypeCollection...construction of RAS pathway gene expression vectors for in vitro and in vivo research. TP53 Mutagenesis Library :...