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We narrowed to 84 results for: pooled crispr library plasmids dna

Showing: 71 - 80 of 84 results

  1. CRISPR Plasmids - Plants

    Type
    Collection
    ... CRISPR Plants CRISPR Plasmids: ...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts The...following CRISPR plasmids have been designed for use in plants. Cut Fully functional CRISPR/Cas enzymes...CRISPR expression plasmids that have been designed for use in plants. CRISPR...: Plants Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional...Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species ...cytosine base editor that can alter DNA bases without inducing a DNA break. Cytosine base editors convert...

  2. Guide to Using Pooled Libraries

    Type
    Guide
    ... Science Guides Guide to Plasmid Pooled Libraries Plasmid Pooled Libraries Jump To...make virus; this creates a pooled lentiviral CRISPR library. Apply pooled library to cells. DIVERGE: Positive... In cDNA libraries, each plasmid contains a unique cDNA. In shRNA or gRNA libraries, each plasmid contains...Overview of what pooled libraries are; how to amplify and use a pooled library; and what types of screens...Introduction to Pooled Libraries Pooled libraries represent a powerful tool for forward genetic screening, or identifying...simplest level, pooled libraries are single preparations of many different plasmids. Plasmids within a given... name, pooled libraries are supplied as a mixed population of plasmids in a single tube. Pooled libraries...

  3. Neurodegeneration Research Collection

    Type
    Collection
    ... Elife. 2023 Oct 24. See More CRISPR Tools Find CRISPR pooled libraries available from Addgene, sortable...find our guide to using plasmid pooled libraries . New and Noteworthy: Use a CRISPRi system to target alpha-synuclein... and plasmid collections that may help with your neurodegeneration research. Disease Info Plasmid Collection...Research Tools Neurodegeneration Plasmid Collection A collection of plasmids expressing wild type or mutant...Subcellular Localization, Optogenetics Plasmids, and Chemogenetics Plasmids. New and Noteworthy: Explore NeuroPAL...more. Plasmids provided by the foundation can be found on our Michael J. Fox Foundation Plasmid Resource...Find out about the plasmid collections available at Addgene that may be useful for neurodegeneration ...

  4. CRISPR History and Development for Genome Engineering

    Type
    Collection
    ...like... CRISPR Guide CRISPR Protocols gRNA Design Tools CRISPR Blog Posts The CRISPR revolution shows no...out Addgene’s CRISPR Guide and CRISPR blog posts , or find CRISPR plasmids for your research. Addgene has... CRISPR CRISPR History CRISPR History and Development for Genome Engineering You may also like...stretches of DNA known as CRISPR arrays. Separate Cas proteins then express and process the CRISPR loci to ...mechanisms to block CRISPR. Some prevent the CRISPR-Cas complex from binding to DNA. Others inhibit the...target CRISPR to a given locus makes it especially amenable to genetic screens. Pooled gRNA libraries can...sequencing, CRISPR is a robust system for genome-wide screening. CRISPR Technological Advances CRISPR technology...

  5. CRISPR Plasmids - Epigenetics

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    Collection
    ... CRISPR Epigenetics CRISPR Plasmids: Epigenetics Browse CRISPR Plasmids By Function Genome...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts To...maintained by the Doudna Lab . Browse, sort, or search the tables below for CRISPR plasmids for epigenetic...CRISPR plasmids for targeted epigenetic modification, including histone acetylation/demethylation, and...Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species ...Mammalian ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Plant ID Plasmid Gene/Insert...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...

  6. CRISPR Plasmids - Repress Gene Expression

    Type
    Collection
    ... CRISPR Interfere CRISPR Plasmids: Interfere...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Catalytically...maintained by the Doudna Lab . Browse, sort, or search the tables below for CRISPR plasmids for transcriptional...CRISPR plasmids for transcriptional repression of target genes. CRISPR...Interfere Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional...Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species ...Mammalian ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Bacteria ID Plasmid Gene/Insert...

  7. CRISPR Plasmids - Activate Gene Expression

    Type
    Collection
    ...Activate CRISPR Plasmids: Activate Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Catalytically...maintained by the Doudna Lab . Browse, sort, or search the tables below for CRISPR plasmids for transcriptional...CRISPR plasmids for activating or upregulating gene expression in various model organisms. CRISPR...Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species ...Mammalian ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Bacteria ID Plasmid Gene/Insert...Publication Drosophila ID Plasmid Gene/Insert Promoter PI Publication Plant ID Plasmid Gene/Insert Promoter...

  8. CRISPR Plasmids - gRNAs

    Type
    Collection
    ... CRISPR gRNAs CRISPR Plasmids: gRNAs Browse CRISPR Plasmids By Function Genome Editing...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts The...Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species ... any of these gRNA plasmids and review the publication associated with each plasmid for more information...PAM sequence. Which CRISPR application is this gRNA sequence compatible with? CRISPR knockout experiments...and unpublished plasmids containing gRNA sequences designed to be used with any CRISPR nuclease and function...information on the experimental use of these plasmids. ID Plasmid Gene/Insert Vector Type PI Publication Do...

  9. CRISPR Guide

    Type
    Guide
    ...screening using CRISPR libraries What Are Pooled Lentiviral CRISPR Libraries? Pooled lentiviral CRISPR libraries...post on lentiviral pooled libraries . Browse Libraries: CRISPR Pooled Libraries Cas9 Fusion Tools dCas9 cannot...using CRISPR plasmids in your lab, from knockouts to pooled library screening. Science...Figure 7: Overview of CRISPR transposases Browse Plasmids: CRISPR transposases CRISPR Recombinases and Integrases...genome-wide screens using CRISPR involves pooled lentiviral CRISPR libraries. Figure 8: Overview of genome-wide...gene, so CRISPR libraries contain thousands of unique gRNAs. gRNA design for CRISPR libraries is usually...For more detail on using CRISPR libraries for screens, see our pooled library guide and our blog post ...

  10. CRISPR Plasmids - Tagging

    Type
    Collection
    ...and Myers Tagging Plasmids Protein Species Tag Donor Plasmid gRNA plasmid gRNA plasmid FLAG pFETCh_Donor...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts The...CRISPR plasmids for endogenous tagging of your gene of interest. CRISPR... CRISPR Protein Tagging CRISPR Plasmids...Plasmids - Protein Tagging Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit...Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species ...well as the plasmids and protocols needed for using them, are described below. Read our CRISPR 101 post ...
Showing: 71 - 80 of 84 results