...because different components of the specimen will refract and reflect the light. These differences create... of biological research and are found in many different types of laboratories. These tools allow you to...sizes - from phone-sized, foldable microscopes that aren't very powerful (but are cheap and accessible) to... light microscope with labels. Created with BioRender.com. Base Light Source Condenser and Diaphragm Stage...objective performs essentially the same task but has different magnifying powers. When you look at the side of...

...may need to be adjusted to accommodate slight differences between products. Addgene does not endorse or... Inclusion of this information is solely for transparency intended to support reproducibility in science...temperature or 37 °C. The protocol notes when there are different incubation options available. Caution Use the ...76322-134 Pipettes, 10 mL, VWR 89130-898 1 L polystyrene bottle, Corning 430518 PBS, 1X pH 7.4, VWR 45000...and vortex. Pro-Tip You may need to try a few different concentrations of antigen to determine the ideal...into the first three wells of each row, using a different standard solution for each row of a 96-well ELISA...

...glutaGRO, Corning 25-015-CI) Heat-inactivated FBS Polybrene (10mg/mL), Millipore TR-1003-G 1X PBS pH 7.4 without...21-040-CV (cations can affect the attachment of adherent cells) 0.45 μm polyethersulfone filter, Nalgene...5 mL of 100X glutaGRO. Store at 4 °C. Pro-Tip Different brands and lots of FBS can promote or inhibit ...lentivirus into DMEM complete containing 10 μg/mL polybrene. Note, this protocol was developed using low titer...Volume of DMEM complete (μL) Volume of 10mg/mL polybrene (μL) 1:10 150 1348.5 1.5 1:25 60 1438.5 1.5 1:...

...days. Note that growth rate differences can be inferred by the differences in colony size. Figure 2: Cas9...21-040-CV (cations can affect the attachment of adherent cells) Trypsin EDTA (TrypLE, Thermo Fisher 12605...increased serum concentrations, may be optimal for different cell types. Before starting this procedure, be...expression. This protocol has been optimized for adherent cells but can be modified for suspension cells...

...Vortex Reagents Adherent HEK293T cells (ideally AAV-293T clones) Pro-Tip While adherent, these cells are...Vector Guides Virus Blog Posts Molecular Biology Reference Introduction This protocol can be used to produce...1272 cm 2 Heat-inactivated FBS (HI-FBS) Pro-Tip Different brands and lots of FBS can promote or inhibit ...21-040-CV (cations can affect the attachment of adherent cells) 1 mg/mL Polyethylenimine (PEI) 25 kDa MW...

...backbone (See Addgene's Sequencing Primers for reference). Many sequencing cores have a list of common ...recommends using Addgene's sequencing results as a reference for primer design. A good sequencing reaction ...reaction with high background: If you obtain a different sequence than expected and wish to contact Addgene... should I do? Check your trace file first; the apparent mismatch/mutation may be the result of a mis-called...

...accurately sized on a gel and also such that they are different enough in size to be easily resolved from each...approach, it is often a good idea to choose two different enzymes that will give you unique but distinct...In the example below we want to know how to differentiate between two clones that differ only in the ...the expected products from each orientation are different sizes. Simply run the digest products on a gel...

...two different enzymes for adding an insert into a vector (one enzyme on the 5' end and a different enzyme...controls. See Tips and FAQ below for details. Try different vector to insert ratios to optimize the ligation...will tell you exactly how much of each to use. Reference Page Top Index...

...isothermal reaction. With the activities of three different enzymes, the product of a Gibson Assembly is a...mix (isothermal reaction mix) consists of three different enzymes within a single buffer mixture and an ...primers to include in the Gibson reaction are different than with normal PCR primers (Gibson et al. , ... than five fragments at a time. Resources and References New England Biolabs (NEB) (Link opens in a new...

...use one or the other, but there is very little difference between the two. Note: Make sure to use the same... buffer as the one in the gel box (do not mix different buffers and do not use water). Microwave for 1...be in the top portion and your bands will be differentially intense. If this happens, you can just soak...to load 1.0 μg in 10 μL, make 1.1 μg in 11 μL. Reference Page Top Index...

...plasmid Resuspension buffer Denaturing solution Renaturing solution 2 mg/mL RNase A TE or water-saturated..., including plasmid DNA, into solution. Add a renaturing solution to the denatured bacteria. Note: This...Solution II at room temperature Solution III - Renaturing Solution (Potassium Acetate) 120 mL 5M Potassium...included in the resuspension buffer of most kits. Reference Page Top Index...

...SDS-PAGE gel tank. Pro-Tip Different SDS-PAGE gel tanks will have different gel orientations. Refer to...may need to be adjusted to accommodate slight differences between products. Addgene does not endorse or... Inclusion of this information is solely for transparency intended to support reproducibility in science...

...Vector Guides Virus Blog Posts Molecular Biology Reference Introduction This protocol describes how to use...1814040 Microseal adhesive seal, Bio-Rad, MSB1001 Polystyrene Reservoirs, VWR, 89094-662 Microcentrifuge tubes... the NTC. Pour the 1X dilution buffer into a polystyrene reagent reservoir. Using a 20–200 µL multichannel...cartridge. Add 800 µL of droplet generation oil to a polystyrene reagent reservoir. Using the 20–200 µL multichannel...

...Tetracycline 10 µg/mL Tips and FAQ What is the difference between high copy and low copy plasmids? The ...hr on average. Certain features of a plasmid may render it low copy regardless of plasmid size. See the...350 - 400 rpm to obtain a higher cell density. Reference Page Top Index...

...career path, differences in research culture between the US and Australia, and advice for current graduate...Specialist Jane Hannon discusses her career path, current projects in the works, and all of the things she...

... at specific sequences. There are hundreds of different restriction enzymes, allowing scientists to target...(such as a digestion with another enzyme in a different buffer), but will not be gel purifying it, you... not cut your insert, you could try using two different enzymes that have compatible sticky ends. See...

... This approach can be adapted for different cell lines and different transfection reagents. Workflow Timeline...5 mL of 100X glutaGRO. Store at 4 °C. Pro-Tip Different brands and lots of FBS can promote or inhibit ...

...method. The protocol may need to be optimized for different cells, target proteins, etc. Sharing speeds science...may need to be adjusted to accommodate slight differences between products. Addgene does not endorse or... Inclusion of this information is solely for transparency intended to support reproducibility in science...

...Collection Antibodies Blog Posts Molecular Biology Reference General Transfection Protocol Recombinant Antibody...may need to be adjusted to accommodate slight differences between products. Addgene does not endorse or... Inclusion of this information is solely for transparency intended to support reproducibility in science...

... to use, you can check out our Empty Backbone Reference . Background Subcloning by restriction digest ... a fusion protein) Ideally, you will find two different restriction enzymes for your subcloning. It is...expression vector and can begin your studies. Reference Page Top Index...