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Showing: 281 - 320 of 414 results

  2. Single-cell tracking of lineage and identity with CellTag

    Type
    Blog Post
    ...indexing strategy that simultaneously tracks clonal history and cell identity at the single-cell level, thus...track the dynamics of gene expression and clonal history associated with changes in cell identity. The Morris...

  3. Multifunctional Neural Fibers: Modulation from Gut to Brain

    Type
    Blog Post
    ... polymers that can work with all the bells and whistles. The fibers are made from a process called thermal...additional components are added - the ‘bells and whistles’ – including microscale light emitting diodes,...

  5. GCE4All: Making Genetic Code Expansion Accessible

    Type
    Blog Post
    ...technology or as a therapeutic.  A (very) brief history GCE came onto the synthetic biology scene in 1989... V. W. (2021). Genetic Code Expansion: A Brief History and Perspective. Biochemistry, 60(46), 3455–3469...

  7. Antibodies 101: Fab Fragments

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    Blog Post
    ...technique for monoclonal antibodies.  J Histochem Cytochem : Off J Histochem Soc 34(6):703–706. https://doi.org...

  9. Antibodies 101: Secondary Antibodies

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    Blog Post
    ...standard approach is the direct method. ELISAs, immunohistochemistry, and immunocytochemistry may all use either...the need for a secondary antibody. Other immunohistochemistry advances have found ways to increase signals...

  10. CRISPRainbow and Genome Visualization

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    Blog Post
    ...the 4D nucleome. Visualizing specific loci has historically been difficult, as techniques like fluorescent...this rainbow of colors, Ma et al. performed sophisticated tracking of multiple chromosomal loci in live...

  11. Gendered Innovations: Why Does Sex of the Cell Matter?

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    Blog Post
    ...blogger Londa Schiebinger, PhD, Hinds Professor of History of Science, Stanford University. Sex and gender... work to be done! Researchers need to learn sophisticated methods of sex and gender analysis. Universities...

  12. Hot Plasmids - October 2020

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    Blog Post
    ...2020.07.02.185249 Predicting cell cycle length with a histone-fused fluorescent timer By Kate Harten DeMaio Studies...color-changing fluorescent timer (FT) protein to the histone H2B that allows for  observation in live cells....

  13. CRISPR in the Clinic

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    Blog Post
    ...is caused by a variety of single nucleotide polymorphisms (SNPs) in the TTR gene and is molecularly characterized...Plasmids and Resources CRISPR 101 eBook CRISPR History and Development Resources on the Addgene blog...

  15. Light Sheet Fluorescence Microscopy

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    Blog Post
    ...that also allows antibody penetration for immunohistochemical labeling in whole tissue (15).  CLARITY is...236.
 Pubmed. P.A Santi. (2011). Journal of Histochemistry & Cytochemistry, 59, 129-138. Pubmed. J. Huisken...

  16. Deep Dive: Fixing and Permeabilizing for Immunofluorescence

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    Blog Post
    ...some integral membrane proteins. Journal of Histochemistry & Cytochemistry 33:813–820. https://doi.org... Tissue Fixation & the Nature of Fixatives in Histology. Leica Biosystems. https://www.leicabiosystems.com...

  17. Antibodies 101: Producing Recombinant Antibodies

    Type
    Blog Post
    ...applications for validation include western blot, immunohistochemistry, and immunocytochemistry. It’s important...applications. An antibody that performs well in immunohistochemistry may not perform well in a western blot. ...

  18. MXS Chaining

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    Blog Post
    ...Subcellular localization 1 TagBFP 399nm/ 456nm histone 2B (H2B) Chromatin 2 Cerulean 433nm/ 475nm Lyn-tag... 3. Gibson DG, Young L, Chuang RY, Venter JC, Hutchison CA, Smith HO.Enzymatic assembly of DNA molecules...

  20. March for Science

    Type
    Blog Post
    ...edition. Cambridge university press, 1988. AAS: Historical Trends in Federal R&D NJ.com: Rush Holt: Lawmakers...affected by Trump’s immigration ban Studies in History and Philosophy of Science Part: Democratic values...

  21. Site Directed Mutagenesis by PCR

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    Blog Post
    ... PCR followed by Restriction Fragment Length Polymorphism (RFLP) analysis (right). Introduction of restriction...of that particular site with fragment length polymorphism (RFLP) analysis. In this process, if your mutation...

  22. Antibodies 101: Validation

    Type
    Blog Post
    ...This is a good option for applications like immunohistochemistry on human tissues, when (1) other strategies...correspond to protein levels, so you perform immunohistochemistry with your antibody on these tissues (B)....

  24. Antibodies 101: Introduction to Antibodies

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    Blog Post
    ...Antibodies can be used to visualize tissues (immunohistochemistry) or cells (immunocytochemistry). This could...Arbabi-Ghahroudi M (2017) Camelid Single-Domain Antibodies: Historical Perspective and Future Outlook. Front Immunol...

  25. Fluorescent Proteins 101: Photoactivatable Fluorescent Proteins

    Type
    Blog Post
    ...reversible photoactivation - and have enabled sophisticated imaging techniques.     Irreversible photoactivation...victoria) GFP (wtGFP) by mutating Threonine 203 to Histidine (T203H) (1). wtGFP normally contains a mixed population...

  27. It’s CRISPR Week at Addgene!

    Type
    Blog Post
    ... today, we’ll start with a CRISPR overview and history. Tuesday will focus on generating knockouts using...

  28. The Addgene Antibody Data Hub

    Type
    Blog Post
    ...application — flow cytometry, western blot, immunohistochemistry, immunocytochemistry, ELISA — but in all...

  33. What's New in CRISPR - Spring 2019

    Type
    Blog Post
    ...Feng Zhang lab identified a Cas12b from Bacillus hisashii, BhCas12b that is functional at 37 °C but preferentially...
Showing: 281 - 320 of 414 results