We narrowed to 242 results for: gRNA
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TypeCollection...You may also like... CRISPR Guide CRISPR Protocols gRNA Design Tools CRISPR Blog Posts RNA-Guided Nucleases...consist of two components: a short ~100 nt guide RNA ( gRNA ) that uses 20 variable nts at its 5’ end to base...the target DNA. The Joung lab recently described gRNA and Cas9 expression vectors and validated the use...design of short oligonucleotides needed to construct gRNA expression vectors. More information about RGNs ...
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CRISPR Plasmids - RNA Targeting
TypeCollection... dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila...CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Type VI CRISPR systems... -
Editor's Choice, August 2016
TypeBlog Post...the transitions at target sequences specified by gRNAs. Caveats, potential specificity issues, and other... -
CRISPR Plasmids - CRISPR Transposases (CAST)
TypeCollection... dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila...CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts CRISPR-associated... -
CRISPR Pooled gRNA Libraries
TypeCollection... dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila...Type Species PI Lentiviral Generation gRNAs per gene Total gRNAs Adamson DNA Repair CRISPRi Libraries ...Reduced Double-gRNA Library 137999 Knockout Human Parts 3rd 3 59,576 Human Whole Genome sgRNA iBAR Library...CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts CRISPR is a useful...experiments, due to the relative ease of designing gRNAs and the ability to modify virtually any genetic ...thousands of plasmids, each containing multiple gRNAs for each target gene. In a CRISPR screening...libraries require the use of lentivirus to deliver the gRNA/Cas9 to target cells. Therefore, you must be equipped... -
What's New in CRISPR - September 2019
TypeBlog Post...lab. The Bikard library contains ~93,000 unique gRNAs that target random positions along the E. coli MG1655...targeting requires two sgRNAS: one that targets the knock-in site and a universal sgRNA that targets the donor... the genomic loci of interest. As the universal sgRNA is not predicted to target sites in the human, pig... -
Build Your CRISPR Vocabulary
TypeBlog Post...spacer and the remaining sequence as the scaffold. gRNA: "Guide RNA" is a general term referring to the ...require a tracrRNA. sgRNA: “Single-guide RNAs” were invented by synthetic biologists. An sgRNA combines a crRNA... tracrRNA pair or an sgRNA. It is often used interchangeably with the term sgRNA. Seed sequence: The 8...complementary DNA sequences. In laboratory settings, an sgRNA includes the crRNA and tracrRNA sequences in a “... on the 3’ end of an RNA guide (either crRNA or sgRNA). Cas9 requires perfect complementarity in this ... -
Celebrating Outstanding CRISPR/Cas9 Achievements at the Dr. Paul Janssen Award Dinner
TypeBlog Post..., programmable DNA cutting enzyme. Second, since gRNA and crRNA could be combined into one RNA to direct... -
Viral Vectors 101: What is a Virus (and a Viral Vector)?
TypeBlog Post...the case of retroviruses, reverse transcribe their gRNA into DNA, which is then transcribed into mRNA before... -
Viral Vectors 101: Parts of the AAV Transfer Plasmid
TypeBlog Post...smaller CRISPR-Cas proteins such as SaCas9, CRISPR gRNAs, or your gene of interest. Promoter The promoter... -
Using Addgene's New Viral Service for Your Research
TypeBlog Post...nickase, Cas9 activators, Cas9 repressors, select gRNAs, and CRISPR pooled libraries. Whether you’d like... -
Plant Plasmids and Resources
TypeCollection...assembly of one or more gRNA expression cassettes - Qi-Jun Chen. Cas9 and gRNA backbone for use in rice... different functional variants of Cas9 and empty gRNA backbones. A few examples of useful plant CRISPR...Plants includes CRISPR/Cas nucleases, base editors, gRNA backbones, and promoters for expression in monocots... -
CRISPR History and Development for Genome Engineering
TypeCollection...with paired gRNAs to lower off-target cleavage frequency. Truncated gRNAs: Truncated gRNAs display less...You may also like... CRISPR Guide CRISPR Protocols gRNA Design Tools CRISPR Blog Posts The CRISPR revolution... 2: An overview of CRISPR and NHEJ/HDR. The Cas9/gRNA complex produces a DSB at the target genomic locus...is not only simple, but also scalable. Multiple gRNAs targeting multiple loci can be easily expressed ...it especially amenable to genetic screens. Pooled gRNA libraries can be used to identify genes that are...marks. Visualize : Fluorescently labeled dCas9 or gRNAs that bind fluorescent proteins can be used to image...labeling of genomic loci with dCas9 and engineered sgRNAs using CRISPRainbow. Nat Biotechnol . . PMID: 27088723... -
Three Tips for Preventing Viral Plasmid Recombination in Your Samples
TypeBlog Post... Z, Ma X, Wang N, Huo Y-X (2020) Reversed paired-gRNA plasmid cloning strategy for efficient genome editing... -
Hot Plasmids - September 2019 - CRISPR, Auxin Inducible Degradation, MerMAID Channelrhodopsins, and Genome Looping
TypeBlog Post...call the LADL Anchor. Using specifically designed gRNAs, they can then recruit these LADL Anchors to genomic... -
Lentiviral Prep Service
TypeCollection...RNA guide (gRNA) that harbors targeting sequences. Browse our repository for a list of gRNA plasmids targeting... Doench , Root 73179 Human gRNA pooled library in lentiCRISPRv2 Human sgRNA library in backbone lentiCRISPRv2...pooled libraries 73178 Human sgRNA library Brunello in lentiGuide-Puro Human sgRNA library in backbone lentiGuide-Puro...Human CRISPRa sgRNA library Calabrese in backbone XPR_502 (P65 HSF), Set A Human CRISPRa sgRNA activation...containing 56,762 unique sgRNAs. This backbone does not contain dCas9. 56,762 unique sgRNAs targeting 18,885 ...pooled libraries 73633 Mouse sgRNA library Brie in lentiGuide-Puro Mouse sgRNA library in backbone lentiGuide-Puro...targeting 19,114 genes and containing 76,441 unique sgRNAs along with 1000 non-targeting controls. This backbone... -
Pushing the Limits of DNA Assembly
TypeBlog Post...Addgene.org Browse Our CRISPR Resources Find Validated gRNAs Browse All Plasmids ...thousands of base pairs. Cloning sgRNAs Generating constructs to produce sgRNA for CRISPR experiments can be... plating, screening and generation of sgRNA. Generation of sgRNA libraries, with maximum complexity, is...constructs with multiple point mutations Cloning sgRNAs Production of Yeast Expression Cassettes Generating...synthetic oligo to generate a single-guide RNA (sgRNA). Using only one single-stranded oligo, instead ...to create a double-stranded oligo. Generating an sgRNA construct becomes as simple as combining your favorite... -
Improved Plasmid Maps Powered by SnapGene
TypeBlog Post...commonly used for Golden Gate cloning or for CRISPR gRNA cloning. With this more functional display, you... -
CRISPR 101: Cytosine Transversion Editors
TypeBlog Post... Acronym Full name Notes CRISPR terms gRNA/sgRNA Guide RNA RNA used by Cas9 to locate a matching... the DNA strand that does not base pair with the gRNA. Base editors target this “non-target strand.” To... predicting CGBE editing outcomes and assisting sgRNA selection is available at http://www.sunlab.fun:... -
The CRISPR Software Matchmaker: A New Tool for Choosing the Best CRISPR Software for Your Needs
TypeBlog Post...Resources at Addgene Get Tips on How to Design a gRNA Read Our CRISPR Guide to Catch up on All the CRISPR...Read Other Blog Posts about CRISPR Find validated gRNAs ...of what determines good sgRNA design. Incidentally, the first software for sgRNA design (by Hsu et al.,...technical expertise, thoughtful single guide RNA (sgRNA) design, and the abnormally low GC content of the...without computational support. Indeed, rational sgRNA design is not possible without relying on some kind...end of 2014, I began developing software to make sgRNA design accessible to all. At the time I thought ... Breaking down the barriers: My view on current sgRNA design tools The goal of this post is to provide...