We narrowed to 323 results for: sars
-
TypeBlog Post...Advanced functions: These functions are not entirely necessary but should be considered extremely useful, depending...experiment being pursued. You should also look at the glossary section of the CRISPR Software Matchmaker, it ...
-
CRISPR Methods for Bacteria: Genome Engineering, CRISPRa, CRISPRi, Base Editing, and More
TypeBlog Post...across multiple gRNAs per gene (Singh et al. 2016). Sarah Fortune's lab M. tuberculosis CRISPRi plasmids Noting...proteotoxic when used in Mycobacterial species, Sarah Fortune’s lab screened 11 other Cas9 orthologs to... -
Protocol - How to Design Primers
TypeProtocol...Primer Design for PCR Oligonucleotide primers are necessary when running a PCR reaction. One needs to design... -
Weighing Reagents Protocol
TypeProtocol...scoopula or spatula is clean, and sterile (if necessary), to prevent any potential contamination. Scoop... -
Water Bath Protocol
TypeProtocol...way, as items are floating, you do not need to necessarily maneuver the bottles or tubes to identify them... -
Protocol - How to Inoculate a Bacterial Culture
TypeProtocol...used to grow up sufficient numbers of bacteria necessary to isolate enough plasmid DNA for experimental... -
Lab Safety for Biosafety Levels One and Two
TypeProtocol... any spills immediately, and decontaminate as necessary. For large spills that cannot be easily cleaned... -
Protocol - How to Ligate Plasmid DNA
TypeProtocol...into a 10μL reaction, scale the reaction size as necessary - being sure to increase the amount of buffer ... -
22 Hot Plasmid Technologies from 2014
TypeBlog Post...under the stringently low expression conditions necessary to avoid biological perturbation in a variety ... additional iterations of Level 1 assembly if necessary. Combining multiple Level 2 vectors in the final... -
AAV Purification by Iodixanol Gradient Ultracentrifugation
TypeProtocol... density gradient media, it is reportedly not necessary to remove iodixanol prior to use of your purified... -
Pouring LB Agar Plates
TypeProtocol... in 500 mL bottles. The extra empty volume is necessary to prevent your molten agar from boiling over ... -
CRISPR Library Amplification
TypeProtocol...genome, for example. Amplification is usually necessary to produce sufficient quantities of library for... -
Twenty Years of Sharing Science!
TypeBlog Post...Today marks Addgene's 20th anniversary! We've done so much in the past two (2!) decades that it's hard... -
AAV Packaged on Request is Here!
TypeBlog Post...After an order is placed, we’ll facilitate the necessary MTA, then start production and quality control... -
Plasmid Grant to Aid Cervical Cancer Screening in Ghana
TypeBlog Post...using plasmids in creative ways that we wouldn’t necessarily have expected when we originally sent out the... -
2022 and 2023: Reflections and Looking Forward
TypeBlog Post...Addgene lies in our future. As we near our 20th anniversary, we have many successes to reflect upon, a wealth... -
Plasmid Preps: Different Purity, Different Quantities, Different Uses
TypeBlog Post...microorganism. The amount of plasmid DNA that is necessary for bacterial transformation of chemically and... -
More Data for You: Find Articles Citing Addgene Plasmids
TypeBlog Post...feature to help save you from performing any unnecessary cloning - the list of "Associated Plasmids" from... -
Stepping into a Virtual Internship: My Experience at Addgene
TypeBlog Post...unexpected decisions. As someone who was not necessarily a fan of the sciences, I knew I would run into... -
Top 10 Open Science Developments of 2013
TypeBlog Post...peer-reviewed journal publications, although many don’t necessarily publish in OA journals themselves. A good number...