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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 1,052 results for: sta

Showing: 141 - 160 of 1052 results

  1. Serotype Testing AAV

    Type
    Collection
    ...tropism of different serotypes. In addition to the standard 100 µL sample size, the vectors listed below are...

  2. Adenovirus Plasmids

    Type
    Collection
    ...transient transgene expression, as well as the substantial immune response induced by the viral capsid. ...

  3. CRISPR Plasmids - Yeast

    Type
    Collection
    ...Plasmid Promoter Cloning Enzyme(s) Validated In Resistance Co-expressed Cas9 Depositing lab Cas9 species...

  4. AAV Molecular Tools

    Type
    Collection
    ...Expression of human codon-optimized Cas9 from Staphylococcus aureus (SaCas9). 6 Zhang Labeling Tools These...

  8. TALEN Plasmids and Kits

    Type
    Collection
    ...improved, truncated TALE backbone architecture as established by Miller et al. (PMID: 21179091), as well as...

  9. The Pleiades Promoter Project

    Type
    Collection
    ...Promoter Project (Link opens in a new window) was established to advance research and therapies for brain disorders...

  10. Caltech Systemic Capsids

    Type
    Collection
    ... (Link opens in a new window) . As part of our standard viral production , these viral vectors undergo...

  12. CRISPR Pooled gRNA Libraries

    Type
    Collection
    ...169082 Knockout Mouse Turner N/A 10 13,500 Mouse Metastasis CRISPR-Cpf1(Cas12a) Double Knockout Library 123361...

  15. Gamma-Retroviral Vector Guide

    Type
    Guide
    ...as NEB Stable cells. These strains reduce the frequency of homologous recombination of unstable regions...of a transgene. Stable Cell Lines Gamma-retroviral vectors are often used to make stable cell lines. Like...markers, such as the puromycin resistance gene, conferring antibiotic resistance to infected host cells. When...and replicate. This feature can be harnessed for stable delivery of various genes, mutations, or treatments... in a variety of cell types and models, such as stable long-term expression in a host and low immunogenicity...specific to each virus type. From an experimental standpoint, the main difference between lentiviruses and...into the transfer plasmid can be done with most standard cloning methods, including restriction enzyme,...

  16. Molecular Biology Reference

    Type
    Guide
    ...populations, an antibiotic resistance gene (i.e., a gene whose product confers resistance to ampicillin) is included...efficiently in a laboratory setting. Stable - Plasmids are stable long-term either as purified DNA or ...dideoxy sequencing. To understand Sanger sequencing, we first need to understand DNA replication. DNA is... GUA, GUG Start AUG* Stop UAG (amber), UGA (opal), UAA (ochre) *AUG is the most common start codon. Alternative...purposes of isolating one specific DNA sequence. This obstacle has been overcome by the field of recombinant ...providing a benefit to the host, such as antibiotic resistance. This benefit can be context-dependent, and thus...their use of studying and manipulating genes. For instance, most plasmids are replicated in E. coli and are...

  17. Lentiviral Vector Guide

    Type
    Guide
    ...as NEB Stable cells. These strains reduce the frequency of homologous recombination of unstable regions...and clinical tools. Stable Cell Lines Lentiviral vectors can be used to make stable cell lines due to their...markers, such as the puromycin resistance gene, conferring antibiotic resistance to infected host cells. When...and replicate. This feature can be harnessed for stable delivery of various genes, mutations, or treatments...Lentiviral vectors can target non-dividing cells, enable stable long-term expression in a host, and exhibit low...contain packaging genes. From an experimental standpoint, the main difference between lentiviruses and...into the transfer plasmid can be done with most standard cloning methods, including restriction enzyme,...

  18. Antibody Guide

    Type
    Guide
    ... the constant region, and is shown in dark blue and dark orange. The constant region is a stable part ...antibody’s constant region - specifically, it is determined by a region called the “heavy chain constant region...techniques use a nuclear stain, such as DAPI, to identify individual cells. These stains also emit fluorescent...sequencing, and enables the variable region and constant regions of the antibody to be independently manipulated...Structure Figure 1: Structure of an antibody A standard antibody is made up of four protein chains: two...responsible for producing antibodies, can change the constant region, and therefore change the isotype of antibodies...capable of recognizing and binding epitopes on constant regions of other antibodies. This has useful research...

  19. CRISPR Guide

    Type
    Guide
    ...used to install the LSR Bxb1’s landing site into the genome at a desired locus, then Bxb1 installs the donor...including resistances to chemotherapy drugs and toxins, cell viability, and tumor metastasis. One of the...genome-wide screens. This guide will provide a basic understanding of CRISPR biology, introduce the various applications...applications of CRISPR, and help you get started using CRISPR in your own research. CRISPR Basics In this...target cleavage, while mismatches toward the 5′ end distal to the PAM often permit target cleavage. Generating... Cas9 orthologs are available. SaCas9 (from Staphylococcus aureus ) has a coding sequence ~1 kb shorter...addition of a structured motif to enhance pegRNA stability PEmax - optimized PE architecture; increased synergy...

  20. Adeno-associated virus (AAV) Guide

    Type
    Guide
    ...as NEB Stable cells. These strains reduce the frequency of homologous recombination of unstable regions...overcome this obstacle, has been to use smaller Cas9 orthologs, such as SaCas9 from Staphylococcus aureus ....into the transfer plasmid can be done with most standard cloning methods, including restriction enzyme,...Gateway cassette containing the ccdB gene, a ccdB-resistant strain is necessary. For more information on cloning...AAVs cannot enter the lytic cycle, and instead establish latent infections by integrating into the host...years without damaging the host cell. Episomal stability allows for long-term transgene expression in non-dividing...well characterized and are often preferred over standard rAAV vectors for in vivo applications. Other Engineered...
Showing: 141 - 160 of 1052 results