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Showing: 141 - 160 of 1049 results
  1. CRISPR Plasmids and Resources

    Type
    Collection
    ...nickase enzymes fused to reverse transcriptase install new genetic information into a specific DNA site...
  2. CRISPR Plasmids - C. elegans

    Type
    Collection
    ...gRNA Plasmid Promoter Cloning Enzyme(s) Delivery Resistance Co-expressed Cas9 Depositing lab Cas9 species...
  3. Serotype Testing AAV

    Type
    Collection
    ...tropism of different serotypes. In addition to the standard 100 µL sample size, the vectors listed below are...
  4. AAV Molecular Tools

    Type
    Collection
    ...Expression of human codon-optimized Cas9 from Staphylococcus aureus (SaCas9). 6 Zhang Don’t See What You...
  5. Adenovirus Plasmids

    Type
    Collection
    ...transient transgene expression, as well as the substantial immune response induced by the viral capsid. ...
  6. CRISPR Plasmids - Yeast

    Type
    Collection
    ...Plasmid Promoter Cloning Enzyme(s) Validated In Resistance Co-expressed Cas9 Depositing lab Cas9 species...
  7. TALEN Plasmids and Kits

    Type
    Collection
    ...improved, truncated TALE backbone architecture as established by Miller et al. (PMID: 21179091), as well as...
  8. Caltech Systemic Capsids

    Type
    Collection
    ... (Link opens in a new window) . As part of our standard viral production , these viral vectors undergo...
  9. The Pleiades Promoter Project

    Type
    Collection
    ...Promoter Project (Link opens in a new window) was established to advance research and therapies for brain disorders...
  10. CRISPR Pooled gRNA Libraries

    Type
    Collection
    ...169082 Knockout Mouse Turner N/A 10 13,500 Mouse Metastasis CRISPR-Cpf1(Cas12a) Double Knockout Library 123361...
  11. Gamma-Retroviral Vector Guide

    Type
    Guide
    ...as NEB Stable cells. These strains reduce the frequency of homologous recombination of unstable regions...of a transgene. Stable Cell Lines Gamma-retroviral vectors are often used to make stable cell lines. Like...markers, such as the puromycin resistance gene, conferring antibiotic resistance to infected host cells. When...and replicate. This feature can be harnessed for stable delivery of various genes, mutations, or treatments... in a variety of cell types and models, such as stable long-term expression in a host and low immunogenicity...specific to each virus type. From an experimental standpoint, the main difference between lentiviruses and...into the transfer plasmid can be done with most standard cloning methods, including restriction enzyme,...
  12. Molecular Biology Reference

    Type
    Guide
    ...populations, an antibiotic resistance gene (i.e., a gene whose product confers resistance to ampicillin) is included...efficiently in a laboratory setting. Stable - Plasmids are stable long-term either as purified DNA or ...dideoxy sequencing. To understand Sanger sequencing, we first need to understand DNA replication. DNA is... GUA, GUG Start AUG* Stop UAG (amber), UGA (opal), UAA (ochre) *AUG is the most common start codon. Alternative...purposes of isolating one specific DNA sequence. This obstacle has been overcome by the field of recombinant ...providing a benefit to the host, such as antibiotic resistance. This benefit can be context-dependent, and thus...their use of studying and manipulating genes. For instance, most plasmids are replicated in E. coli and are...
  13. Lentiviral Vector Guide

    Type
    Guide
    ...as NEB Stable cells. These strains reduce the frequency of homologous recombination of unstable regions...and clinical tools. Stable Cell Lines Lentiviral vectors can be used to make stable cell lines due to their...markers, such as the puromycin resistance gene, conferring antibiotic resistance to infected host cells. When...and replicate. This feature can be harnessed for stable delivery of various genes, mutations, or treatments...Lentiviral vectors can target non-dividing cells, enable stable long-term expression in a host, and exhibit low...contain packaging genes. From an experimental standpoint, the main difference between lentiviruses and...into the transfer plasmid can be done with most standard cloning methods, including restriction enzyme,...
Showing: 141 - 160 of 1049 results