Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

We narrowed to 1,414 results for: sta

Showing: 141 - 160 of 1414 results

  2. CRISPR Plasmids - Cascade-Cas3

    Type
    Collection
    ...Cas3. After nicking the non-targeted strand, Cas3 starts unraveling DNA using its helicase properties, cleaving...

  3. Brzezinski Lab CRISPR Collection

    Type
    Collection
    ...Benchling or using similar design tools and ordered as standard desalted oligonucleotides with the appropriate...

  4. Resolute Plasmid Collection

    Type
    Collection
    ...fluorescence, mass/charge properties, protein thermal stabilization, and changes of voltage all contribute to assess...

  5. CRISPR Plasmids and Resources

    Type
    Collection
    ...nickase enzymes fused to reverse transcriptase install new genetic information into a specific DNA site...

  6. Serotype Testing AAV

    Type
    Collection
    ...tropism of different serotypes. In addition to the standard 100 µL sample size, the vectors listed below are...

  7. E11 Bio PRISM Collection

    Type
    Collection
    ...Resources Protein-barcode Reconstruction by Iterative Staining with Molecular annotations (PRISM) is a set of...

  8. Recombinases AAV Preps

    Type
    Collection
    ...Wilson 196410 AAV-GfaABC1D-Cre-4x6T GfaABC1D none 5 Stanley Thomas Carmichael 214848 AiP13780: pAAV-AiE0743m...

  11. AAV Molecular Tools

    Type
    Collection
    ...Expression of human codon-optimized Cas9 from Staphylococcus aureus (SaCas9). 6, 8 Feng Zhang Labeling Tools...

  12. TALEN Plasmids and Kits

    Type
    Collection
    ...improved, truncated TALE backbone architecture as established by Miller et al. (PMID: 21179091), as well as...

  13. The Pleiades Promoter Project

    Type
    Collection
    ...Promoter Project (Link opens in a new window) was established to advance research and therapies for brain disorders...

  14. Caltech Systemic Capsids

    Type
    Collection
    ... (Link opens in a new window) . As part of our standard viral production , these viral vectors undergo...

  16. Plasmids 101: Gram Negative and Positive Bacteria

    Type
    Blog Post
    ...chemistry behind Gram’s crystal violet stain and the ion interactions that cause the stain retention in gram...positive bacteria trap the crystal violet stain within their membrane, while the stain is washed from the gram...realized that certain stains were retained only in some species of bacteria. The stain colored one type, ...Gram’s discovery, adding a second stain, safranin, as a counterstain to help visualize the bleached bacteria...Procedure The stain is a pretty simple process. First, flood the heat-fixed smear with the crystal violet stain... bacteria will be stained blue or purple, and gram negative bacteria will be stained red or pink, as shown...mixing the stains and solutions used, check out these resources.   Bruckner, M. Gram Staining. https://...

  17. Antibodies 101: Flow Cytometry

    Type
    Blog Post
    ...Direct staining is quicker than indirect staining and eliminates potential non-specific staining that may...its native state.  Antibodies-based flow cytometry can use both direct and indirect staining methods. In...intracellular staining where binding of large immune complexes may be hindered. Indirect staining can be beneficial...fixation. If staining both cell surface and intracellular proteins in parallel, then stain the surface ... non-specific staining.  If you’re using antibodies for detection, include an unstained control with cells...cells that went through the staining procedure minus the antibody. An unstained control allows you to detect...-specific staining associated with the primary antibody. When following an indirect staining protocol,...

  18. Illuminating Choices: A Guide to Selecting Fluorescent Dyes and Ligands

    Type
    Blog Post
    ... electrons are elevated from a ground state to an excited state through the absorption of photons. After... elevation in this excited state, the electron returns to its ground state by emitting a photon with slightly...lengths of time, the stability of fluorescence over time is crucial. For instance, the fluorescence of...toxicity and high photostability is crucial. These properties ensure a more consistent and stable signal over...Fluorescence refers to the emission of light by a substance (fluorophore) that has absorbed light or electromagnetic...al., 2014). Other dyes may prove challenging to stain intracellular compartments in in vivo model systems...so extra care must be taken to assess if proper staining is achieved. Fluorescent ligands have more flexibility...

  19. Antibodies 101: Isotypes

    Type
    Blog Post
    ...which heavy chain constant domain it pairs with its variable chain domain. B cells start by co-expressing...expressed with the IgM and IgD constant domains because they are the first constant domain genes in the cluster...heavy-chain constant genes μ and ẟ. These are the first two genes in the heavy-chain constant domain gene...heavy-chain constant domain genes, placing the variable domain genes next to a different constant domain gene... antibody that’s determined by its heavy-chain constant region (see Antibodies 101: Introduction to Antibodies...antibody isotypes that each have a unique heavy-chain constant region: IgM, IgD, IgG, IgE, and IgA.    ...labeled with Fc, Fab, heavy chain, light chain, constant region, and variable region.   Why are antibody...

  20. Deep Dive: qPCR

    Type
    Blog Post
    ...Amplification Efficacy Assumptions Standard Curve Yes Standard Curve No experimental sample variation...variation ΔΔCT No Standard Curve reference=target Pfaffl et al No Standard Curve sample=control...deep dive series, which aims to increase your understanding and technical proficiency with common applications...in protein expression. (If you're just getting started, check out our Introduction to PCR.) But if you're...you're ready to go beyond the 101 and take your understanding to the next level, read on for more in-depth... differences of twofold or greater with good statistical significance (Taylor, 2017). With the right conditions...sometimes cause as many issues as it solves, so understanding and using the application correctly is imperative...
Showing: 141 - 160 of 1414 results