Addgene

We narrowed to 395 results for: AGA

Showing: 321 - 360 of 395 results

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The PAM Requirement and Expanding CRISPR Beyond SpCas9

Type

Blog Post

...NNNNRYAC Streptococcus thermophilus (St) NNAGAAW Treponema denticola (Td) NAAAAC ~20 additional...
Four Base Editing Reporters to Monitor and Enrich Editing in Real-time

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Blog Post

...D, Serebrenik A, Shaban N, Brown WL, Donati F, Munagala U, Conticello SG, Harris RS (2018) A fluorescent...
Binning Singletons: Tackling Conference Networking When You Don’t Know Anyone

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Blog Post

...Iwasa, Shawnna Buttery, Kat Milligan-Myhre, and Pragati Chengappa. Financial and moral supporters Denise...
CRISPR-based Genome Editing Systems For Editing in E. coli

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Blog Post

...Increasing rounds of transfer on transposition-inducing agar lead to higher cargo copy numbers. (D) The effect...
AAV Titers: Where Do They Come From And What Do They Mean?

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Blog Post

...reliable, or if the sample needs to be quantified again. Some of the issues with titrating by qPCR are ...
Sonic Hedgehog? Sleeping Beauty? Learn About the Genes Behind Addgene’s Conference Room Names

Type

Blog Post

...squash" originated from the squash itself, but think again. Spaghetti squash is actually a component of the...
Designing Your Chalk Talk for the Academic Job Interview

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Blog Post

...favor a different model. Finding evidence for or against a model is a standard scientific strategy. However...
Changing Labor Laws Bring Increased Postdoc Wages

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Blog Post

...as of October 1st the salary minimum was raised again to $51,600, itself a raise of 3.2%. Other institutions...
Deciphering the Mysteries of Behavior with Viral Vectors

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Blog Post

...open new ways for a novel therapeutic approach against drug addiction. Anxiety tests Rodents have a natural...
Antibodies 101: Reading a Flow Plot

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Blog Post

...different populations using conjugated antibodies against CD11c and CD123. You can see that mDCs are CD11c...
Bioinformatics at Addgene

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Blog Post

...QC'ing data at Addgene is like standing under a Niagara Falls sized rainbow Over the years we have...
Plasmids 101: E. coli Strains for Protein Expression

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Blog Post

...of several popular strains of E. coli for DNA propagation. While great for cloning purposes, these E. coli...
Tips for Getting a Faculty Position

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Blog Post

...create a new field, impact disease treatment, etc., again in clear terms. "Implications for human disease"...
Hot Biosensors 2022: Year-End Roundup

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Blog Post

...kinetics and high signal/noise ratio. Here, Takeharu Nagai and colleagues present an improved genetically encoded...
Antibodies 101: Normalization and Loading Controls for Western Blots

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Blog Post

...Ah, the notorious western blot: we meet again. So useful, yet so finicky to design and optimize. Today...
Antibodies 101: Introduction to Gating in Flow Cytometry

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Blog Post

... populations with basically a smear in between. Again, it can depend on the type of stain whether a tighter...
An Integrin Antibody Toolkit from IPI

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Blog Post

...αVβ3.13, may also block function of mouse αVβ3, again the first known reagent to do so. The 15 hybridoma-derived...
Filming Science Videos in the Age of Social Distancing

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Blog Post

...what works and what doesn’t, and trying all over again. Here’s that process! Figure 1: The Design-Build-Test-Learn...
Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection

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Blog Post

...PMID: 29505611. PubMed Central PMCID: PMC5837189. Nagai, Takeharu, et al. "Expanded dynamic range of fluorescent...
Deep Mutational Scanning with One Pot Saturation Mutagenesis

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Blog Post

...in the same orientation as those in the plasmid. Again, if they are in the opposite direction, both strands...
3 Tips to Improve HDR Efficiency for CRISPR Editing in Human Cells

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Blog Post

...template that corrects the blocking mutation and again identify the right clone from a few hundred. From...
Five Popular Model Organisms, Part 2

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Blog Post

... to be replicated. Because E. coli are easily propagated and have a generation time of around 20 minutes...
Site Directed Mutagenesis by PCR

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Blog Post

...fragments should be separated on a high percentage agarose gel (~3%). Note that primer duplication will evade...
Evolution of Lab Techniques

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Blog Post

...restriction enzymes and then running the products on an agarose gel allows a researcher to properly orient specific...
Viral Vectors 101: AAV Serotypes and Tissue Tropism

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Blog Post

...Diep, J., Ishikawa, Y., Jae, L. T., Wosen, J. E., Nagamine, C. M., Chapman, M. S., & Carette, J. E. (2016...
CRISPR 101: Epigenetics and Editing the Epigenome

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Blog Post

...experimental period, as this mark was faithfully propagated in culture and in vivo. In certain cases, epigenetic...
Antibodies 101: The Basics of Western Blotting

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Blog Post

...blocked, it is stained with a primary antibody against your protein of interest. But which antibody should...
Scientific Reproducibility - Focusing on Solutions at the Minisymposium on Reproducibility

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Blog Post

...directly by creating well characterised antibodies against all human extracellular proteins with community-based...
Finding Your Perfect Job After University

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Blog Post

...Working in industry: virology On completing my MSc, I again had to look at what type of work I would like to...
A Practical Guide to Optimizing AAV DIO and FLEx Vector Expression

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Blog Post

...j.ymthe.2004.05.024 Dudek AM, Pillay S, Puschnik AS, Nagamine CM, Cheng F, Qiu J, Carette JE, Vandenberghe LH...
When Fidelity Matters: A frank discussion about ligase fidelity

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Blog Post

...DNA ligase, are naturally able to discriminate against ligating substrates containing base pair mismatches...
Transferable Skills Guide: Teamwork

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Blog Post

...matter whether that other person really is acting against the team’s interests or not; once that seed of ...
Quick Guide to All Things Lentivirus

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Blog Post

...multiplicity of infection (MOI=ratio of virus to cells). Again, my tip here is to test several protocols as the...
Lambda Red: A Homologous Recombination-based Technique for Genetic Engineering

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Blog Post

...temperature allows the repressor to re-nature and again repress expression of exo, beta and gam. A major...
Sequencing Options for CRISPR Genotyping

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Blog Post

...equitable society. Read more at https://magazine.scienceforthepeople.org/. References 1. Fu, Yanfang, et...
Antibodies 101: Designing Your First Flow Panel

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Blog Post

...briefly go through each section of this article again. In the beginning, we talked about the relationship...
Custom CRISPR Screens & the Green Listed Software

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Blog Post

...Another interesting approach is to design screens against known druggable targets, preferentially those for...
Hassle-free 96-well Format Epitope Tagging Using Cas9 Ribonucleoprotein

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Blog Post

...complexes - from the cell-lysate using antibodies against the target protein. The captured protein complexes...
A Primer on Optogenetics: Introduction and Opsin Delivery

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Blog Post

...for sufficient opsin to reach the axon terminal. Again, this must be empirically determined in your own...
Chemogenetics Guide

Type

Guide

...olanzapine has also been shown to be an agonist against hM4Di, and is especially attractive for use in ...eaav5282. PMID: 30872534 (Link opens in a new window) Nagai Y, Miyakawa N, Takuwa H, Hori Y, Oyama K, Ji B, ...

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