... and transduces the majority of astrocytes and neurons across multiple CNS regions. PHP.eB, an enhanced...Ly6a seemed the most likely candidate due to its strong linkage to the PHP.B phenotype and its expression...references: Dayton, Robert D., Mychal S. Grames, and Ronald L. Klein. "More expansive gene transfer to the ...

... Targeted Neuronal Manipulation. Frontiers in Neuroanatomy, 13. https://www.frontiersin.org/articles/10.3389...

...research for over a century but was brought to the forefront by Thomas Hunt Morgan, who is known as the “father...work with as they can survive in a variety of environmental conditions, and double every 2 hours. Yeast ...Sydney Brenner brought it into the lab to study neuronal development (Brenner, 1973). C. elegans are small...

...neither the aptamer nor the small molecule are strongly fluorescent but the binding of an aptamer to its...investigate intracellular viral DNA dynamics in neuronal cells (Nilaratanakul et al., 2017) and levels ...Jonathan. "RNA fluorescence with light-up aptamers." Frontiers in chemistry 4 (2016): 29. PubMed PMID: 27446908...

... can be from one of the transgene’s endogenous introns or comprised of non-endogenous sequences, such ... A potential solution is to include an in-frame degron, or degradation sequence, upstream of the 5’ splice...Efficiencies of Dual AAV Approaches for Retinal Targeting. Front Neurosci 11: . https://doi.org/10.3389/fnins.2017.00503...

...determination By Mike Lacy Conventional cryogenic electron microscopy (cryo-EM) protocols typically require...in Xenopus nucleosomes and in complex with its chaperone NPM2, but the technique should be widely applicable...marking cell types in the nervous system By Ashley Waldron The mammalian nervous system is composed of myriad...

... Two-photon image of miRFP670nano3-expressing neurons in the neocortex of a living mouse. Image courtesy... and far-red genetically encoded indicators of neuronal activity. J Neurosci Methods 362, 109314 (2021...improved monomeric infrared fluorescent protein for neuronal and tumour brain imaging. Nat Commun 5, 3626 (...

...and Ruddock., 2017), but requires an oxidizing environment to form. When expressed in the non-oxidizing ...cytoplasm. expression in the presence of molecular chaperones that help scFvs fold properly in the cytoplasm...and screened with in vitro assays to select for strong binders. Then the sequences for the variable domains...

...adapted for video making.   Cycle One: The Ironing Board Control Center With the pandemic in full ...that included iPad and her laptop raised on an ironing board, giving it the perfect height (Fig. 2). We...to think of something else.   Figure 2: Ironing boards. Good as a WFH desk, and as a video control...

...controls are equally strong, so the normalized value simply reports which POI band is stronger. The right gel...protein in the lane, your loading control won’t be strongly influenced by unexpected variations in any individual...

...plasma HIV-1, they are at increased risk for other chronic diseases, including dementia, gut disorders, nerve...disease risk is attributed to latent HIV reservoirs, chronic inflammation, and negative metabolic effects of...in this publication at Addgene. Wang Z, Pan Q, Gendron P, Zhu W, Guo F, Cen S, Wainberg MA, Liang C. CRISPR...

...are methylation sensitive. dnaJ Mutation in a chaperonin gene Increases the stability of certain expressed... fhuA (formerly tonA) ferric hydroxamate uptake, iron uptake receptor mutation. T1/T5 Phage resistance...not degraded, cell cannot methylate DNA lac Lac operon mutations Blue/white screening of clones   lacIq...

...confidence that they aren’t simply doing an experiment wrong. However, even simple procedures like pouring a ... easier to learn new experimental techniques. Electronic lab notebooks also play a role here by making...scientists with positive results have done nothing wrong, but the problem is that only these positive results...

...from Yang et al. requires two gRNAs targeting two intronic regions and two repair templates in order to insert... allele requires you to target 2 loxP sites to intronic regions 0.5 - 0.8 kb apart. In the example below...CRISPR/Cas9-based generation of knockdown mice by intronic insertion of artificial microRNA using longer ...

... E. coli or Salmonella, or any cells that bind strongly to nitrocellulose, over-incubation of plates can...pathways for bacteriophage-based therapeutics."Frontiers in Microbiology 7 (2016). PubMed PMID: 27536293...genomic and metagenomic screening." Applied and environmental microbiology 74.5 (2008): 1649-1652. PubMed ...

... was engineered from the cmt and cym bacterial operons. A chimeric transactivator, cTA, was created by...displays low promoter activity (~20% activity of the strong yeast promoter pTEF). As ethanol accumulates, it...sphingomonads and other Alphaproteobacteria. Appl Environ Microbiol. 2013 Nov;79(21):6795-802. doi: 10.1128...

...very hard to speak with individuals who had very strong beliefs in traditional medicine, especially when... reliant on programming skills which are not my strong point. Working in industry: virology On completing...because it is an English speaking country with a strong economy and plenty of jobs in science at world ...

...co-workers who want to make our organization as environmentally friendly as possible. We worked with Andrew... unless we manage to make Addgene the most environmentally friendly organization on the planet, in which...easier. Trust Ultimately, your team could have a strong sense of purpose, an excellent leader, and perfectly...

...carrier of genetic information and led to the coronation of DNA as the molecule of life.  This was further...genetic engineering experiment which goes horribly wrong. The plot of the movie involves The Rock and his....111.131433 Doudna JA, Charpentier E (2014) The new frontier of genome engineering with CRISPR-Cas9. Science...

...e.g. an R01 from the NIH, in the United States) Strong letters of reference that attest to your talent...person makes time for you, as needed, this is a strong indicator that this person cares about your science... to finding out you've been doing something all wrong or that a reagent has not been convincingly demonstrated...

...and how do they come off and on in a cellular environment? If these are pressing issues in your laboratory...majority of FRET acceptors are delivered to the wrong address in a different city, you can still develop...list Acceptor labeling: Sometimes it's okay to be wrong In contrast to FRET donors, absolute FRET acceptor...

...pMD2.G VSV-G envelope expressing plasmid Didier Trono pVSV-G Expresses VSV-G envelope protein for...are used in the lab? Check out our “Rabies and Neuronal Tracing” blog post! You can also browse tools ...

...lentivirus envelope plasmid pMD2.G from Didier Trono’s lab! Lentivirus envelope vector pMD2.G This plasmid...protocols and information, be sure to check out the Trono Lab website. If you have plasmids you’d like to ...

...around the world!) that have kept Addgene at the forefront of life sciences research for the past decade....not just for a better day at the office but for stronger science as well. This blog is our way of continuing...

...spatial, cellular, metabolic, and cytokine microenvironment that exists in vivo. Over the past five years...cell type, in more complex and physiological microenvironments. These screens have primarily been performed...

...developing a strong graduate student community and the need for belonging in the scientific environment 3:09 ...

...spatially with Cre-recombination. Read the paper in Frontiers in Molecular Neuroscience Find the AAV-based...can be used in diverse bacteria from various environments. It has the advantage of stable genomic integration...

...they have a graduate degree in science. I find it ironic that we do all of our training as scientists (5...jobs (that is what networking is about), but be upfront about interest in a job. Ask openly to be connected...

...activity and geotactic behavior (Biron et al., 2005).   References Biron, D. G., et al. "Behavioural manipulation...

...liberated to adorn the walls of universities and the front pages of newspapers. Science is now being adapted...while - a tool to expand perception, which is ironically what science is about. For me it is essential...

...results in the degeneration of spinal cord motor neurons. This disease is estimated to affect anywhere from...for use in the clinic and thereby restore motor neuron function in spinal muscular atrophy patients. Noushin...

...important to this function. With the welcoming environment on my team and at Addgene, I felt comfortable...seemed. The transition into a traditional work environment was aided by the efforts being put in by Addgene...

...increasingly accessing websites other than through the ‘front door,’ the homepage is still important. It needs...easily. In the redesign, the new search bar is now front and center, with a clear call to action - Search...

... tobacco, Arabidopsis, and corn. For both β-glucuronidase (GUS) and GFP reporters, as well as multiple...Transgenesis with GreenGate Plasmids Interview: Nicola Patron on Plant Synthetic Biology, MoClo, and More Plasmids...

...test recombination efficiency. When expressed in neuronal cells, each line showed broad recombinase-dependent...hamster ovary (CHO) cells, as well as in cortical neurons in layer 2/3 of the mouse brain. Rubin’s work shows...

...vehemently about the need for change.  Dr. Bourne strongly believes this is an amazing time of promise for... Novartis industry postdoctoral program.  His strongest comments were about the astounding lack of reproducibility...

...interested in meeting movie stars, but when I met the astronaut Jim Lovell I was speechless (the club of people...students in their labs and the importance of the strong, cross-disciplinary collaboration they shared to...

...of input material, cells or tissue, to produce a strong enough signal over background noise. This is not...protocol, the most significant being the usage of a strong detergent to permeabilize cells rather than to ...

...that there are many ways your experiments can go wrong. Whether it be a bad fridge freezing your samples...editing and later looking for songs and sounds to synchronize with the images thereby making the videos more...

...CRISPR is a sleek acronym for a real mouthful of a phrase: Clustered Regularly Interspaced Short Palindromic...parts! If you’re still a bit confused by CRISPR acronyms, this post is for you. We’ll cover the common ...