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We narrowed to 562 results for: Spr

Showing: 161 - 180 of 562 results

  1. CRISPR Plasmids for Genomic Visualization

    Type
    Collection
    ...list of CRISPR resources by visiting our CRISPR reference page . CRISPR Guide Viral Preps CRISPR Protocols... about visualization using CRISPR and other CRISPR topics, read our CRISPR Guide . Mammalian ID Plasmid... CRISPR Visualize CRISPR Plasmids:... using CRISPR. Created with BioRender.com. Browse, sort, or search the tables below for CRISPR plasmids...Publication Return to top CRISPR Resources Addgene has a large selection of CRISPR plasmids and resources...Find more CRISPR functions along with plasmids categorized by organism by visiting our CRISPR plasmids ...CRISPR/Cas plasmids for labeling and visualization of genomic loci. CRISPR...

  2. CRISPR Plasmids - Mammalian Expression

    Type
    Collection
    ...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Base...CRISPR plasmids that have been designed for use in mammalian systems. CRISPR... CRISPR Mammalian Expression CRISPR...CRISPR Plasmids: Mammalian Expression The following CRISPR plasmids have been designed for use in mammalian...mammalian expression systems. Cut Fully functional CRISPR/Cas enzymes will introduce a double-strand break (...Promoter Selectable Marker PI Publication Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit...->G (or T->C on the opposite strand) mutations. CRISPR-X, a base-editing system from the Bassik lab, uses...

  3. Zhang Lab CRISPR Page

    Type
    Collection
    ... Genome Engineering CRISPR Zhang Lab CRISPR Plasmids Zhang...Zhang Lab CRISPR Plasmids Available from Addgene Zhang Lab CRISPR Resources Tips for and FAQs about the ...Zhang Lab CRISPRs Browse All Zhang Lab Plasmids Zhang Lab Website You may also like... CRISPR Guide CRISPR...phages and plasmids. CRISPR loci in microbial hosts contain a combination of CRISPR-associated (Cas) genes...specificity of the CRISPR-mediated nucleic acid cleavage. We have harnessed the type II CRISPR nuclease system...backbone cloning information: CRISPR-Cas9 mouse toolbox protocol 637.8 KB CRISPR-Cas9 Cre expression vectors...CRISPR Cas9 gRNA plasmids from the Zhang lab Genome...

  4. CRISPR Plasmids - Prime Edit

    Type
    Collection
    ...list of CRISPR resources by visiting our CRISPR reference page . CRISPR Guide Viral Preps CRISPR Protocols... CRISPR Prime Edit CRISPR Plasmids: Prime Edit Mammalian Bacteria...below for CRISPR prime editing plamisds. To learn more about prime editing and other CRISPR topics, read...Ervin Welker Return to top CRISPR Resources Addgene has a large selection of CRISPR plasmids and resources...Find more CRISPR functions along with plasmids categorized by organism by visiting our CRISPR plasmids ...read our CRISPR Guide . Mammalian ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return...Protocols gRNA Design Tools CRISPR Blogs CasPEDIA Content last reviewed: 17 October 2025 Do you have suggestions...

  5. CRISPR Plasmids - Cascade-Cas3

    Type
    Collection
    ...list of CRISPR resources by visiting our CRISPR reference page . CRISPR Guide Viral Preps CRISPR Protocols...below for CRISPR Cas3 plasmids. To learn more about Cas3 and other CRISPR topics, read our CRISPR Guide .... CRISPR Cas3 CRISPR Plasmids: Cascade-Cas3...Publication Return to top CRISPR Resources Addgene has a large selection of CRISPR plasmids and resources...Find more CRISPR functions along with plasmids categorized by organism by visiting our CRISPR plasmids ...Find plasmids for CRISPR-Cas and Cascade for large genomic deletions. CRISPR... Plant Resources Cas3 is a unique member of the CRISPR family. After nicking the target DNA, Cas3 initiates...

  6. Brzezinski Lab CRISPR Collection

    Type
    Collection
    ...Collections Brzezinski Lab CRISPR Collection Brzezinski Lab CRISPR Collection The Brzezinski lab investigates...for gene activation CRISPR/Cas9 Plasmid Variants Figure 1: Schematic of the CRISPR Collection. Plasmids...Find CRISPR plasmids to study the regulatory networks involved in retinal development. Depositor...involved in retinal development the lab utilizes a CRISPR/Cas9 based approach to knockout, repress, and activate...

  7. CRISPR Plasmids - Activate Gene Expression

    Type
    Collection
    ...list of CRISPR resources by visiting our CRISPR reference page . CRISPR Guide Viral Preps CRISPR Protocols...below for CRISPR activation plasmids. To learn more about CRISPR activation and other CRISPR topics, read...Publication Return to top CRISPR Resources Addgene has a large selection of CRISPR plasmids and resources...Find more CRISPR functions along with plasmids categorized by organism by visiting our CRISPR plasmids ...CRISPR plasmids for activating or upregulating gene expression in various model organisms. CRISPR... CRISPR Activate...Activate CRISPR Plasmids: Activate Mammalian Bacteria Drosophila Plant C. elegans Yeast Resources Catalytically...

  8. CRISPR Plasmids - Empty gRNA Vectors

    Type
    Collection
    ...Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts CRISPR requires... CRISPR Empty gRNA Vectors CRISPR Plasmids: Empty gRNA Expression Vectors Browse CRISPR Plasmids...plasmids and therefore are not limited to a single CRISPR function. Alternatively, gRNA-only plasmids can...gRNA sequence, such as BsbI or BsaI Cas9 (or other CRISPR) system that the vector was designed to be used...to be used with. crRNAs used with Cpf1 or other CRISPR systems are also listed here and denoted as such...enzymes? CasPEDIA is an encyclopedia of Class 2 CRISPR systems with wiki entries describing enzyme activity...Enzyme Co-expressed Cas9 Cas9 System Selection PI pCRISPR 42875 Bacteria BsaI none S. pyogenes Chloramphenicol...

  9. CRISPR Plasmids - Repress Gene Expression

    Type
    Collection
    ...list of CRISPR resources by visiting our CRISPR reference page . CRISPR Guide Viral Preps CRISPR Protocols... To learn more about CRISPR interference and other CRISPR topics, read our CRISPR Guide . Mammalian ID... CRISPR Interfere CRISPR Plasmids: Interfere...1: Overview of CRISPR interference. Browse, sort, or search the tables below for CRISPR interference plasmids...Publication Return to top CRISPR Resources Addgene has a large selection of CRISPR plasmids and resources...Find more CRISPR functions along with plasmids categorized by organism by visiting our CRISPR plasmids ...CRISPR plasmids for transcriptional repression of target genes. CRISPR...

  10. CRISPR Plasmids - Purify Genomic Loci

    Type
    Collection
    ...list of CRISPR resources by visiting our CRISPR reference page . CRISPR Guide Viral Preps CRISPR Protocols...more about purification using CRISPR and other CRISPR topics, read our CRISPR Guide . Mammalian ID Plasmid... CRISPR Purify CRISPR: Purify Genomic ... using CRISPR. Created with BioRender.com. Browse, sort, or search the tables below for CRISPR plasmids...Publication Return to top CRISPR Resources Addgene has a large selection of CRISPR plasmids and resources...Find more CRISPR functions along with plasmids categorized by organism by visiting our CRISPR plasmids ...Catalytically inactive CRISPR plasmids for purification of target loci. CRISPR...

  11. CRISPR History and Development for Genome Engineering

    Type
    Collection
    ...like... CRISPR Guide CRISPR Protocols gRNA Design Tools CRISPR Blog Posts The CRISPR revolution shows no...information on CRISPR, check out Addgene’s CRISPR Guide and CRISPR blog posts , or find CRISPR plasmids for... CRISPR CRISPR History CRISPR History and Development for Genome Engineering You may also like...sequencing, CRISPR is a robust system for genome-wide screening. CRISPR Technological Advances CRISPR technology...finger nucleases (ZFNs) and TALENs. CRISPR: An Adaptive Immune System CRISPR (Clustered Regularly Interspaced...prokaryotes used CRISPR as part of an adaptive immune system - utilizing various CRISPR-associated (Cas...of DNA known as CRISPR arrays. Separate Cas proteins then express and process the CRISPR loci to generate...

  12. Bikard Lab - CRISPR Repression Collection

    Type
    Collection
    ...Lab - CRISPR Repression Collection Bikard Lab CRISPR Repression Plasmids You may also like... CRISPR Guide...Guide CRISPR Protocols gRNA Design Tools CRISPR Blog Posts The David Bikard Lab at the Institute Pasteur...a plasmid‐borne CRISPR array coding for guide RNAs (diamonds) interspaced with CRISPR repeat motifs (squares...From the Bikard Lab, CRISPR plasmid collection for modulating expression levels of two targets independently...Pasteur has deposited a collection of CRISPR arrays to modulate the expression levels of two targets independently... DNA-binding protein dCas9 is expressed along a CRISPR array that contains 20 bp "addresses", where dCas9...collection comprises 20 plasmids, each carrying two CRISPR guides. The first one binds to sfgfp with either...

  13. CRISPR Plasmids - Double-Strand Break (Cut)

    Type
    Collection
    ...list of CRISPR resources by visiting our CRISPR reference page . CRISPR Guide Viral Preps CRISPR Protocols... CRISPR Cut CRISPR Plasmids: Cut Mammalian Bacteria... for CRISPR plasmids designed to introduce a DSB. To learn more about NHEJ, HDR, and other CRISPR topics...Publication Return to top CRISPR Resources Addgene has a large selection of CRISPR plasmids and resources...Find more CRISPR functions along with plasmids categorized by organism by visiting our CRISPR plasmids ...CRISPR plasmids that create double strand breaks in DNA. CRISPR...topics, read our CRISPR Guide . Mammalian ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication...

  14. CRISPR Plasmids - Single-Strand Break (Nick)

    Type
    Collection
    ...list of CRISPR resources by visiting our CRISPR reference page . CRISPR Guide Viral Preps CRISPR Protocols...tables below for CRISPR nickase plasmids. To learn more about CRISPR nickases and other CRISPR topics, read... CRISPR Nick CRISPR Plasmids: Nick...Publication Return to top CRISPR Resources Addgene has a large selection of CRISPR plasmids and resources...Find more CRISPR functions along with plasmids categorized by organism by visiting our CRISPR plasmids ...CRISPR nickase plasmids for the generation of single-strand breaks. CRISPR...read our CRISPR Guide . Mammalian ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return...

  15. Protocol - Over-Agar Antibiotic Plating

    Type
    Protocol
    ...learn how to spread antibiotic over-agar. Equipment Pipette tips for both pipetting and spreading Bunsen burner... the spreading; the tip is gently bent to create an “L” shape, and then used like a cell spreader. Several...agar and gently spread over the surface until the liquid is mostly absorbed. The spreading of cells can ... selection of transformed E. coli . Over-agar spreading of antibiotic makes it easy for an investigator... of carbenicillin on top of the agar and gently spread over the surface until the liquid is mostly absorbed... either a bent micropipette tip or other cell spreading device that fits the plate. Incubate plates at...

  16. Protocol - How to Streak a Plate

    Type
    Protocol
    ...objects/solutions. Sterilize your lab bench by spraying it down with 70% ethanol and wiping it down with...cool before touching it to the bacteria. Gently spread the bacteria over a section of the plate, as shown...freshly sterilized loop, drag through streak #1 and spread the bacteria over a second section of the plate... or sterilized loop, drag through streak #2 and spread the bacteria over the last section of the plate...

  17. Video Library

    Type
    Protocol
    ...Deletions with CRISPR Generate genomic deletions in mammalian cells lines using CRISPR/Cas9 Genomic Deletions...Deletions with CRISPR Protocol Over-Agar Antibiotic Plating Create plates to culture bacteria in the lab...
Showing: 161 - 180 of 562 results