We narrowed to 180 results for: car-t

...Ubiquitin pYPQ203 (pMDC32-Ubi1) - Multisite Gateway T-DNA entry plasmid (attR1 & attR2); Zea mays Ubi1 promoter...to isolate, multiply, or express the insert they carry in the target cell. These vectors allow you to test...

...labeling for imaged cells. Image from Morano A, Riedel T, Moshinsky D, 2025. Addgene Report, https://doi.org...expressing Lifeact fused to mScarlet3-S2, mScarlet3-M163H (mScarlet3-H), or mScarlet3. Reproduced from Ding ...this barrier. The Fu Lab designed mScarlet3-H (also known as mScarlet3-M163H), inspired by crystal structures...resulting in mScarlet3-S2 (M163H/M66Q; the "S" is for "stable") (Ding et al., 2026). mScarlet3-S2 and mScarlet3...proteins in identical conditions. Find mScarlet3-S2 and mScarlet3-H plasmids here! Ding, Y., He, W., Wang... on to learn more about: Highly photostable mScarlet3 variants Addgene's own AAV2 and AAV5 packaging...Exceptionally stable variants of red fluorescent protein mScarlet3 By Mike Lacy One of our most popular red fluorescent...

.... PubMed Central PMCID: PMC5171807. 6. Machleidt, T., et al. (2015) NanoBRET-A novel BRET platform for...see the movies demonstrating Ca2+ dynamics in cardiomyocytes. Enhanced Nano-Lantern constructs are available...

... spinoculation, has been found very effective for T cells transduction for instance. I hope you will ...laboratory. The risk of generating RCL is low but extra care has to be taken especially if you are working with...of interest.   Advantages Disadvantages Can carry large transgenes (>8 kb) Potential for generation...feature for your experiment; it must be chosen carefully. Often researchers want a simple set-up where ...oncogenes for instance should be manipulated with care. See the NIH’s biosafety guidelines for more information...

...applications, like chimeric antigen receptor T cell (CAR-T) therapy, the location of integration can greatly...integration and its effect on clinical pre-infusion CAR T-cell products. Journal of Translational Medicine... M., Alvarado, A. F., Moffatt, T. N., Edavettal, J. M., Swaminathan, T. A., & Braun, S. E. (2021). HIV-based...doi.org/10.2174/1566523054546224 PMID: 16101513 Dull, T., Zufferey, R., Kelly, M., Mandel, R. J., Nguyen, ... 33981779 Kim, H. S., Hwang, G., Lee, H. K., Bae, T., Park, S., Kim, Y. J., Lee, S., Park, J., Bae, S.... Hartenian, E., Shi, X., Scott, D. A., Mikkelsen, T. S., Heckl, D., Ebert, B. L., Root, D. E., Doench,..., Y., Liu, H., Lu, A., Ma, J., Cai, Y., Fuksenko, T., Pelayo, A., Shah, N. N., Kochenderfer, J. N., Norberg...

...disorders. Delivery of chimeric antigen receptor T cell (CAR-T) therapy is a particularly popular application...vector purification by high-speed centrifugation for CAR T cell manufacturing. Molecular Therapy — Methods ...options, including: Gibbon Ape Leukemia Virus (GALV) — T and B cell transduction Murine Leukemia Virus (MuLV...M. C., Varshney, G. K., Gildea, D. E., Wolfsberg, T. G., Baxevanis, A. D., & Burgess, S. M. (2014). MLV...doi.org/10.1093/nar/gkt1399 PMID: 24464997 Maetzig, T., Galla, M., Baum, C., & Schambach, A. (2011). Gammaretroviral...should be made on a case-by-case basis. Retroviruses carry a slightly elevated risk of integrating into proto-oncogenes...Naseer, A., De Ridder, J., Allahyar, A., Wang, W., Carpenter, E., Mason, A., Wong, G. K., Cameron, E. R., Kilbey...

...projects include investigating negative regulators of T cells and cytokine signaling in tumor immunity. When...fluorophores and their emission spectra. Some fluorophores carry their emission peaks within their names, for example...

...fuGFP) 395 503 Monomer pUS252 - Mammalian Expression T-Sapphire 399 511 26 4.9 1.3 h Prone to dimerization...Expression mScarlet 569 594 71 5.3 2.9 h Monomer pmScarlet_C1 - Mammalian Expression pCS2+mScarlet-C Cloning...Expression mCarmine 603 675 5.81 5.58 Monomer mCarmine in pcDNA3 - Mammalian Expression mCarmine in pRSETB...Maturation Structure Plasmids mScarlet-H 551 592 15 4.8 4.4 h Monomer pmScarlet-H_C1 - Mammalian Expression...mApple-pBAD - Bacterial Expression mScarlet-I3 568 592 68 4.2 2 min Monomer pmScarlet-I3_C1 - Mammalian Expression...pWXC078 - Worm Expression mScarlet3 569 592 78 4.5 37 min Monomer pmScarlet3_C1 - Mammalian Expression pWXC077...Vector - Mammalian Expression mScarlet-I 569 593 57 5.4 36 min Monomer pmScarlet-i_C1 - Mammalian Expression...

...INTEGRATE ( I nsertion of T ransposable E lements by G uide R NA- A ssisted T argeting), developed by Samuel...Topkar, V. V., Nguyen, N. T., Zheng, Z., Gonzales, A. P. W., Li, Z., Peterson, R. T., Yeh, J. J., Aryee, M...PMID: 28041849 Sakuma, T., Nishikawa, A., Kume, S., Chayama, K., & Yamamoto, T. (2014). Multiplex genome... R. T., Silverstein, R. A., Amrani, N., Peng, C., Kramme, C., Savic, N., Pacesa, M., Rodríguez, T. C.,... (22), 5635-5652.e29. PMID: 34653350 Chu, V. T., Weber, T., Wefers, B., Wurst, W., Sander, S., Rajewsky... Gao, L., Cox, D. B. T., Yan, W. X., Manteiga, J. C., Schneider, M. W., Yamano, T., Nishimasu, H., Nureki...Y., Nakada, S., Yamamoto, T., Sano, S., Hotta, A., Takeda, J., & Mashimo, T. (2019). CRISPR-Cas3 induces...

...Ying Chen, Robert C. Robbins, Mark A. Kay, Michael T. Longaker, and Joseph C. Wu. "A nonviral minicircle... by loxP sites. While there is only a small loxP scar behind at the site of excision, this approach is...insertional mutagenesis, the presence of a genetic scar, and incomplete silencing of transgenes. Overall...antigen-1 (EBNA1) based episomes: These plasmids carry the origin of replication (oriP) element and the...5. González, Federico, Stéphanie Boué, and Juan Carlos Izpisúa Belmonte. "Methods for making induced pluripotent... pluripotent stem cells: reprogramming à la carte." Nature Reviews Genetics 12, no. 4 (2011): 231-42. ...Mouse Expression Vectors Human Expression Vectors Carey et al Hockemeyer et al Brambrink et al Maherali...

...end should have low stability – at least one A or T between position 15-19. Avoid targeting introns. Avoid...Ampicillin VWR: #7177-48-2. Use at 100 μg/mL. Carbenicillin VWR: #80030-956. Use at 100 μg/mL. C.2 Annealing...agar plates containing 100 μg/mL ampicillin or carbenicillin (an ampicillin analog). Due to the long terminal... ligation into LB + 100 μg/mL ampicillin or carbenicillin. Day 2: 2. Spin down the cultures and use a ... to 55°C. Add 1mL of 100mg/mL ampicillin or carbenicillin to obtain a final concentration of 100 μg/mL...distribution of any literature by Addgene is not meant to carry with it, and does not grant any license or rights...distribution of materials by Addgene is not meant to carry with it, and does not grant any license, express...

..., C, or T K G or T M A or C N A, T, C, or G R A or G S C or G V A, C, or G W A or T Y C or T Amino Acids...Guanine T Thymine U Uracil Single Letter Code: Ambiguous bases Nucleobase B C, G, or T D A, G, or T H A, ...A), Thymine (T), Cytosine (C) and Guanine (G). In the double helix, A always pairs with T and C always...thymine, cytosine, and guanine (abbreviated to A, T, C, and G, respectively). The bases are organized ...DNA form hydrogen bonds, where A always pairs with T, and C always pairs with G. The order of nucleotides...vitro DNA replication in addition to the normal A, T, C, or G nucleotides. The four standard bases (dNTPs... each time a base is added. Again, each base (A,C,T, or G) is labeled with a different color, making it...

..., X. P., Slocum, S. T., DiBerto, J. F., Xiong, Y., Urushihata, T., Hirabayashi, T., Fujimoto, A., Mimura...PMID: 25937170 Weston, M., Kaserer, T., Wu, A., Mouravlev, A., Carpenter, J. C., Snowball, A., Knauss, S....Prakash, M., Ikefuama, E. C., Friedman, N., Brown, T. M., Lipscombe, D., Moore, C. I., Hochgeschwender,...Farrell, M. S., Pei, Y., Wan, Y., Yadav, P. N., Daigle, T. L., Urban, D. J., Lee, H. M., Sciaky, N., Simmons...Sysa-Shah, P., Rodriguez, L. A., Ellis, R. J., Richie, C. T., Harvey, B. K., Dannals, R. F., Pomper, M. G., Bonci...Inoue, K. I., Kumata, K., Seki, C., … Minamimoto, T. (2020). Deschloroclozapine, a potent and selective...071013-014048 PMID: 25002280 Strader, C. D., Gaffney, T., Sugg, E. E., Candelore, M. R., Keys, R., Patchett...

...26289977 (Link opens in a new window) He, T. C., Zhou, S., da Costa, L. T., Yu, J., Kinzler, K. W., & Vogelstein... 21453277 (Link opens in a new window) Bulcha, J. T., Wang, Y., Ma, H., Tai, P. W. L., & Gao, G. (2021...Bullard, B. L., Corder, B. N., Gordon, D. N., Pierson, T. C., & Weaver, E. A. (2020). Characterization of a..., A. J., Gilbert, S. C., Hill, A. V. S., & Lambe, T. (2017). Chimpanzee adenoviral vectors as vaccines...: 29083948 (Link opens in a new window) Geisbert, T. W., Bailey, M., Hensley, L., Asiedu, C., Geisbert..., A., Lee, M. J., Wolf, J. M., Reid, R. R., & He, T. C. (2017). Adenovirus-mediated gene delivery: potential...Haydon, R. C., Kinzler, K. W., Vogelstein, B., & He, T. C. (2007). A protocol for rapid generation of recombinant...

...ChR2; increased photocurrent amplitude 450–470 C1V1(t/t) ChR1-VChR1 chimera with E122T and E162T mutations...F., Davidson, T. J., O'Shea, D. J., Sohal, V. S., Goshen, I., Finkelstein, J., Paz, J. T., Stehfest, K...-Benson, A., Cho, Y. K., Morimoto, T. K., Chuong, A. S., Carpenter, E. J., Tian, Z., Wang, J., Xie, Y...., V., Matthews, G. A., Acker, L. C., Sørensen, A. T., Young, A., Klapoetke, N. C., Henninger, M. A., Kodandaramaiah...Beltramo, R., Scanziani, M., Hegemann, P., Oertner, T. G., & Wiegert, J. S. (2015). An improved chloride-conducting...VChR1 Red-shifted channelrhodopsin from Volvox carteri 570 Chronos High-speed, red-shifted channel from...channel from Chlamydomonas noctigama ; ChrimsonR carries K176R mutation with increased on/off kinetics 590...

...group on the free 3' T. The topoisomerase acts as a ligase that joins the A and T compatible ends together...complementary base pairs adenine (A) and thymine (T). TOPO cloning utilizes the Taq polymerase, which ... on the 3' end of PCR products. The complementary T comes from a pre-cut, linear, cloning-ready TOPO vector...Restriction enzyme cloning also leaves behind a short scar in the DNA sequence and can be time-consuming compared...entire cloning step (digestion and ligation) can be carried out in one reaction with a single type IIS restriction... the final product, with no undesired sequence ("scar") retained. A disadvantage of type IIS assembly ...

...Link opens in a new window) Matsushita, T., Okada, T., Inaba, T., Mizukami, H., Ozawa, K., & Colosi, P....17763830 (Link opens in a new window) Liu, D., Li, T., Liu, L., Che, X., Li, X., Liu, C., & Wu, G. (2024...Tervo, D. G., Hwang, B. Y., Viswanathan, S., Gaj, T., Lavzin, M., Ritola, K. D., Lindo, S., Michael, S...Huang, C. C., Gerfen, C. R., Schiller, J., Dudman, J. T., Hantman, A. W., Looger, L. L., Schaffer, D. V., ...viral (rAAV) vectors in order to deliver genetic cargo into cells. Recombinant AAV constructs are designed... transgene of interest. The size of the genetic cargo that can be included between the ITRs is limited...for viral vector production, rAAV vectors do not carry the genes to express Rep78 and Rep 68 proteins and...

...Marjanovic, N. D., Dionne, D., Burks, T., Raychowdhury, R., Adamson, B., Norman, T. M., Lander, E. S., Weissman...-0490-7 PMID: 29305085 Hashimoto, M., & Takemoto, T. (2015). Electroporation enables the efficient mRNA... producing stable cell lines, since the genetic cargo (in this case, CRISPR components) integrates into...

...Gadella Calcium Teal genetically encoded calcium sensor T-GECI Blue-shifted genetically encoded Ca 2+ indicator...pH Red pH-sensitive fluorescent protein pHmScarlet . pHmScarlet is a pH-sensitive red fluorescent protein... the sensor's behavior in the target system. Use care when applying a biosensor in a novel context or ...Calcium Red calcium sensor PinkyCaMP PinkyCaMP a mScarlet-based calcium sensor with exceptional brightness...proteins enable metabolic assays at the point of care. Science. 2018 Sep 14;361(6407):1122-1126. Kai Johnsson...-regulatory role of the mitochondrial pyruvate carrier MPC. eLife. 2020 Mar 6;9. pii: 53917. Luis Felipe...sensor modules Grashoff Lab Tension Sensor Plasmids Carsten Grashoff Tension tCRMod tension sensors Tunable...

...CMV ALS Catherine Tomasetto 226554 pRP.ATM c.7865C>T ATM Flag Ataxia telangiectasia Dave Wee 226566 SigmaR1...118744 pScarlet-Kif5a KIF5A mScarlet CMV ALS Beverly Koller 118745 pCAG-Scarlet-Kif5a KIF5A mScarlet CAG ...pENTR221-VPS35-mScarlet VPS35 mScarlet Parkinson's Johanna Ivaska 215474 pEF.DEST51-VPS35-mScarlet VPS35 mScarlet...disease Caren Norden 105971 pME mKate2-dynactin1-811 DCTN1 mKate2 ALS, motor neuron disease Caren Norden...Arrowsmith 184825 pSN837 KIF5A mScarlet CMV ALS Kyoko Chiba 184826 pSN838 KIF5A mScarlet CMV ALS Kyoko Chiba 184827...184827 pSN526 KIF5A mScarlet Strep polH ALS Kyoko Chiba 184832 pSN840 KIF5A mScarlet Strep polH ALS Kyoko...JCE692) PRKCG PKC1 Spinocerebellar ataxia 16 Juan Carlos Igual 89925 Profiline PFN1 T7 ALS Guillaume Romet-Lemonne...