...RNA Targeting RNA Targeting RNA Editing Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled...pyogenes Ventura pCRISPRyl 70007 Other/Yarrowia lipolytica SCR1'-tRNA yes, cut S. pyogenes LEU2 Wheeldon...pyogenes Draetta pCAS1yl 73226 Other/Yarrowia lipolytica yes, cut S. pyogenes LEU2 Yang pLCKO 73311 Mammalian...

...pathway dependencies are extremely important for classification and treatment. On each pathway page, click ... described in the initial Target Accelerator publications, focused on somatic variants found in lung adenocarcinoma...

...RNA Targeting RNA Targeting RNA Editing Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled... changes. ID Plasmid Gene/Insert Promoter PI Publication Empty gRNA Expression Vectors Select a gRNA expression...

...RNA Targeting RNA Targeting RNA Editing Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled... Plasmid Gene/Insert Vector Type Promoter PI Publication Empty gRNA Expression Vectors Select a gRNA expression...

...TCGATGAATTCGAGCTCG–3' ID Plasmid Selectable Marker Tags Publication FP Overexpression Plasmids for constitutive overexpression...marker. ID Plasmid Gene/Insert Selectable Marker Publication Additional Resources Kits MoClo-YTK Yeast Gateway...

...and the DNAs produced by all three methods are identical in sequence. TAL effector repeat arrays produced...sites and then generates individual user-friendly graphical roadmaps for each target that guide the user through...

...well as applied research in small biotech and pharmaceutical companies. The RESOLUTE project has received...interactions represent the framework that provides biological context. Assays monitoring impedance, fluorescence...

...of action, regulation and post-translational modifications, and metabolic pathways and protein interactions...His-tags are cloned at the C-terminus of MDH and, if indicated on the plasmid map, a TEV cleavage site is present...

...Chemogenetics AAV Use our chemogenetics AAV to chemically induce neuronal activity in specific cell types...Gq-P2A-dTomato hM3D(Gq) - Activation dTomato (physically separate) none 1, 9, PHPeB Dimidschstein No available...

... below. Color is used for clarity and does not indicate a specific relationship. The content for this ...Pathway Color is used for clarity and does not indicate a specific relationship. The content for this ...

...Efficient Marker-Free Recovery of Custom Genetic Modifications with CRISPR/Cas9 in Caenorhabditis elegans. ...consortium of biologists and computer scientists dedicated to providing the research community with accurate...

...Proliferating cell nuclear antigen (PCNA) DNA replication foci IPSC cell lines can be ordered from the ... PubMed All Allen Institute for Cell Science Publications (Link opens in a new window)...

...activator-like (TAL) effectors, scientists in several biological disciplines are coming closer to this DNA targeting...DNA regions into a single construct would be technically challenging. Thanks to efforts by the Bogdanove...

...with light. Optogenetics is typically used in neuroscience to control electrical potentials in neurons. See...

... Protocols DNA Quantification DNA Quantification You may also like... Addgene’s DNA Quantification Protocol. Protocols... to protein (260/280) is generally used as an indicator of the purity of DNA samples. These days, many...the NanoDrop, if two consecutive samples have significantly different concentrations, it is possible that...between samples if they are expected to vary significantly in concentration. DNA dissolved in water is ...

...mitochondria produces the energy cells required for chemical reactions. Labeling these structures can be quite...chaperones to help with folding or post-translational modification to function properly. Browse or search the table...

...Kit (one conical is its own Maxiprep). Critical Do not freeze pellets for later purification. Immediately... Protocols CRISPR Library Amplification CRISPR Library Amplification You may also like... Pooled libraries...quantities of library for experimental applications. Repeated amplifications should be avoided as best as possible...origin of replication and antibiotic selection). This recombination, at a low rate, is not typically a problem...Follow this protocol to perform amplification of CRISPR pooled plasmid libraries in Escherichia coli ...refer to our pooled library material pages for amplification protocols that have been developed by the depositor...available. If a pooled library does not yet have an amplification protocol, the following protocol can be used...

...ultracentrifugation. The arrow indicates the 60–40% interface. The vertical black line indicates the location of the... Protocols AAV Purification by Iodixanol Gradient Ultracentrifugation AAV Purification by Iodixanol Gradient...isomolar density gradient medium suitable for virus purification and isolation of cells, organelles, lipoproteins...and use an iodixanol column gradient for AAV purification. Workflow Timeline Day 1: Purify Day 2: Buffer... to collect the solution, or you will lose a significant amount of your virus. When the first fraction..., et al. "Recombinant adeno-associated virus purification using novel methods improves infectious titer...

... Protocols Plasmid Modification by Annealed Oligo Cloning Plasmid Modification by Annealed Oligo Cloning...added when ordering them or can be performed enzymatically later. Experimental Procedure Digest and purify...

...Affinity Purification with Protein A or Protein G Recombinant Antibody Affinity Purification with Protein...: Buffer exchange Equipment Class II, Type A2 Biological Safety Cabinet 4 °C Refrigerator Pipette controller...controller Benchtop centrifuge compatible with 50 mL conical tubes NanoDrop spectrophotometer 37 °C, 5% CO 2...VWR 89130-900 50 mL pipette, VWR 89130-902 50 mL conical tubes, VWR 89039-656 Microcentrifuge tubes, VWR...inhibitors to 1X if this has not already been done. We typically start with about 250 mL of supernatant and add...buffer. re Collect the flow-through in a 50 mL conical tube and dispose after use. Carefully pour the ...closure and loosen cap. Place column in a 50 mL conical collection tube. Centrifuge column at 1000 x g ...