Skip to main content
Addgene
Showing: 161 - 180 of 539 results

  1. Another Pathway into Cells: iTOP

    Type
    Blog Post
    ...stem cells, dendritic cells, embryonic fibroblasts, glia cells and neurons, and cell death post-transduction... Introduction of macromolecules into cultured mammalian cells by osmotic lysis of pinocytic vesicles. ...

  5. Learning Technical Skills at a Lab Central Workshop

    Type
    Blog Post
    ...culture and who was extremely helpful since she was familiar with library generation. Also, I now know what... next workshop, I’ll ask myself: What are the familiar parts of this workshop? What do I understand ...

  6. Science Career Options

    Type
    Blog Post
    ...requires travel can show that you are self organised, reliable, and can work independently. Practical outdoor...are highly desired, as roles are becoming more reliant on technology, and good computer skills will give...

  7. Viral Vectors 101: Gamma-Retroviral Packaging Systems

    Type
    Blog Post
    ...envelope, Phoenix-AMPHO (for the infection of mammalian cells.) Using this system, virus is produced in...of retroviruses for efficient gene delivery to mammalian cells using 293T cell-based systems. Curr Protoc...

  8. Fluorescent CRISPR Reporters: SRIRACCHA and GEmCherry2

    Type
    Blog Post
    ...H2B (histone)-GFP reporter into the genome of mammalian cells.  Figure 1: The SRIRACCHA reporter...–355 . https://doi.org/10.1038/nbt.2842 Wen Y, Liao G, Pritchard T, Zhao T-T, Connelly JP, Pruett-Miller...

  10. Plasmids 101: Yeast Vectors

    Type
    Blog Post
    ...This post, along with a future companion post on mammalian vectors, will catch you up on the core replication...and ability to be used in bacterial, yeast, and mammalian cells.   ...

  11. Plasmid Tools for Microbiome Studies

    Type
    Blog Post
    ...microbiome species such as Snodgrassella alvi and Gilliamella apicoli. The plasmids could also be transformed...Carlotta, et al. "Metagenomic engineering of the mammalian gut microbiome in situ." Nature methods 16.2 (...

  13. CRISPR 101: Cas9 vs. The Other Cas(s)

    Type
    Blog Post
    ...We are all oh so familiar with the nuclease Cas9, but how does it stack up to the new Cas enzymes on ...RNA cleavage and is significantly less toxic to mammalian cells than Cas13 (likely due to the lack of collateral...

  14. Giving gRNAs a Facelift - Synthetic and Beyond

    Type
    Blog Post
    ...are slightly more efficient and less toxic to mammalian cells (Allen, et al., Frontiers in Genome Editing...RNAs to Enhance CRISPR Genome Editing Systems in Mammalian Cells. Frontiers in Genome Editing, 28 (2021)....

  16. Gendered Innovations: Why Does Sex of the Cell Matter?

    Type
    Blog Post
    ...an international research team from Norway and Australia worked with stem cells in mice. They appropriately... of cells and tissues—when that information is reliable—is important to ensuring excellence in basic research...

  19. Mouse Modeling, Part 1: Genetically Engineered Mice

    Type
    Blog Post
    ...are a common model organism used to understand mammalian traits and genetically engineered mouse models...series, I’ll highlight terminology you should be familiar with before working with mouse models, several...

  20. Viral Vectors 101: Chemogenetics

    Type
    Blog Post
    ... However, concerns have been raised over the reliability of using CNO as a DREADD agonist due to its ability...the compound, or compounds, you choose to ensure reliable results (Goutaudier et al., 2019). Using PSAMs...
Showing: 161 - 180 of 539 results