We narrowed to 532 results for: lias

...jGCaMP8 Fast Genetically Encoded Calcium Indicators. Janelia Research Campus. Online resource. 2020 GENIE Project...tethering by PDZD8 regulates Ca 2+ dynamics in mammalian neurons. Science. 2017 Nov 3;358(6363):623-630...Acetyl-CoA PancACe acetyl-CoA sensor for bacterial or mammalian expression, targeted to nucleus, cytoplasm, or... Fluorescent sensor of cyclic AMP (cAMPr) for mammalian or insect expression (Cre-dependent) cAMPr: A ...extracellular L-lactate, in neurons, astrocytes or mammalian cells A genetically encoded fluorescent biosensor...Serotonin Serotonin sensor iSeroSnFR for bacterial or mammalian expression (Constitutive or Cre-dependent) Directed... pii: S0092-8674(21)00374-3. Lin Tian Serotonin Cilia-targeted serotonin sensor GRAB-HTR6 A serotonergic...

...expression. See plasmid 8452 for puromycin resistance. William Hahn 63704 pRetroX GFP T2A Cre CMV/MSV Tetracycline...inducible expression of GFP T2A Cre fusion in mammalian cells. Floris Foijer 47916 pRXTN CMV/MSV A modified...element that increases export to the cytoplasm. William Hahn 11375 MDH1-PGK-GFP_2.0 MSCV Retroviral construct...-Luc MoMSV Stable expression of luciferase in mammalian cells. Alice Wong 83356 pMXs-3XHA-EGFP-OMP25 MoMSV... For tagging mitochondria with HA epitopes in mammalian cells. David Sabatini Return to top Popular Gamma-Retroviral...

...student researchers Arooba Ahmed, Jiachen Lee, and Jillian Parker who published their work on a protein required... Mark Springel, Arooba Ahmed, Jiachen Lee, and Jillian Parker! Special thanks to our sound engineer/producer...

...into plasmids and expressing these in bacteria, mammalian cells, yeast, plants, or insect cells. Benefits...recombinant monoclonal antibodies (R-mAbs) expressed in mammalian cells. Monoclonal antibodies are derived from ...the plasmids are transiently transfected into mammalian tissue culture cells, the R-mAbs are secreted ...antibody heavy- and light-chain DNAs into a single mammalian expression vector A tool kit for rapid cloning...2014 Jul 30;4:5885. Andrew Beavil Cloning and mammalian expression of mouse and human IgG monoclonals ...

...which are used to deliver Cas9 and other genes to mammalian cells. For this reason, the top requested plasmid...envelope protein under the control of the powerful mammalian CMV promoter. Envelope proteins are a key component...

...started with tissue culture The cultivation of mammalian cells in the lab, or tissue culture as it is commonly...called, is a critical tool for many scientists. Mammalian cells provide scientists with the means to study...

...Cell. 2019 Jun 6. Buganim Glial Cells Retinal Ganglion Cells AAV/CRISPR Mouse Glia-to-Neuron Conversion by...and Xeno-Free Culture Conditions: A Clinically Compliant Approach. Stem Cells Transl Med. 2015 Mar 5. pii...Precursor Cells Lentiviral Mouse/Rat Generation of oligodendroglial cells by direct lineage conversion. Nat Biotechnol...into cortical and motor neurons. Unpublished. Ward Glial Progenitor Cells GABAergic Interneurons Lentiviral...Human Direct Conversion of Human Stem Cell-Derived Glial Progenitor Cells into GABAergic Interneurons. Cells...

... Figure 3: Need to introduce plasmids into mammalian cells? Use transfection grade DNA. Transfection...the process of inserting an external plasmid in mammalian cells and it will likely require micrograms of...

...these…. 3. Expect to get 500-700 bases of clean reliable DNA sequence Anything less and you might suspect... Lianna Swanson...

...cloning vector. Lentiviral Plasmids -  If you're familiar with the pLKO plasmids you'll note that those ...expression and pLVTHM, a lentiviral plasmid for mammalian gene silencing. TALENs - Genome engineering methods...

...strategy yields C to A base edits, whereas in mammalian cells, it produces C to G edits. Adenine transversion...recombinase with improved integration efficiencies in mammalian cells. PASTE ( P rogrammable A ddition via S ite-specific...not suitable for all cell types or experiments. Mammalian CRISPR libraries have also been created in AAV...that are poorly transduced by lentivirus. Non-mammalian CRISPR libraries are also available, such as those...examples of activation systems to target genes in mammalian cells include: SunTag system — co-expression of...unique to bacterial cells and does not occur in mammalian cells. SHERLOCK , developed by the Feng Zhang ...protocols Video protocol for genomic deletions in mammalian cell lines Viral service at Addgene Viral plasmids...

...has been shown to work in plant, Drosophila and mammalian cells, and it may find wide use in biological ...efficiently photoconverted in plant, Drosophila, and mammalian cells. Photoconverted GFP is compatible with other...

...’t even answer your question. Does this sound familiar? Well, when it comes to working with Addgene, ...with our new Help Center! Created using the same reliable Zendesk system that powers our email support, ...

...possible to conduct systematic genetic screens in mammalian cells,” David Sabatini, a senior author of one...). Genome-Wide Recessive Genetic Screening in Mammalian Cells with a Lentiviral CRISPR-Guide RNA Library...

...it’s a probiotic strain that can colonize the mammalian intestinal tract and has been used for 100 years... for noninvasive imaging of microorganisms in mammalian hosts. Nature, 553, 86-90. PubMed PMID: 29300010...

... that function in mammalian cells can be used to attentuate RNA levels. In mammalian systems, Cas13a does... RNA Targeting CRISPR Plasmids: RNA Targeting Mammalian Bacteria Plant Resources Type VI CRISPR systems... other CRISPR topics, read our CRISPR Guide . Mammalian ID Plasmid Gene/Insert Promoter Selectable Marker...

...expression system within the Addgene repository is mammalian, but the largest source of unique gene sequences...frequent transfer of genes is from Proteobacteria to mammalian expression vectors. And more broadly, their data...

... can be used to visualize RNA localization in mammalian cells. These tools consist of cobalamin-binding...your own experiments, mCarmine is available in a mammalian expression vector (pcDNA3) and a bacterial expression...

... available for the calcium sensor jGCaMP7 from Janelia, including three jGCaMP7 plasmids now packaged ...iGluSnFR is a useful tool from Loren Looger’s lab at Janelia for neuroscientists to measure glutamate levels...

...were unable to show TtAgo-mediated cleavage in mammalian cells, likely due to high temperature requirements...cleave plasmid and genomic DNA in a variety of mammalian cell lines, with an efficiency similar to that...