We narrowed to 30 results for: CRE-ER

...localize them to the secretory pathway, a dark pool that is mislocalized to the ER can be detected with...blocked from proceeding through the secretory pathway, and retained in the ER. FPs have been undeniably powerful... FP fusion with a resident endoplasmic reticulum (ER) protein instead localizes to the cytoplasm? Or more...absolute position requirements. For example, a KDEL ER retrieval sequence only functions at the extreme ...potential to interfere with the function of resident ER chaperones and may impact cellular function or possibly...complex-localized fusions from properly exiting the ER and fluorescing (see Figure 2) [5].  When using FPs...compose the secretory pathway, endocytic vesicles, mitochondria, lysosomes or they are secreted into the ...

... the ER lumen reaches the sub-millimolar range, much higher than in the cytosol. Thus, for the ER you ... expression, and compatible with constitutive or Cre-dependent expression. These sensors have also been...measuring the events-of-interest. Sensors suitable for ER measurements include: CEPIA (additional versions ... to simultaneously measure calcium changes in the ER, cytosol and mitochondria), the Cameleon-based FRET...binding sites per sensor (to decrease unnecessary calcium buffering and to increase sensitivity), and is a ...fundamental to muscle and nerve function, hormone secretion, blood clotting, and as we all know, a structural...illumination with violet light and (2) exposure to increased intracellular calcium. It has been applied to ...

...fluorescent protein to the Endoplasmic Reticulum (ER). If homodimerization occurs (most likely in an antiparallel...used as positive and negative controls for proper ER structure. Based on these data, we conclude that ...tagging. On the other hand, mScarlet-I shows correct ER labeling, which is good evidence that mScarlet-I ...proteins are available, and their number is steadily increasing. Of course, you want the ‘latest and greatest...

... lab's SARS-CoV-2 sybodies! References Geertsma ER (2013) FX Cloning: A Versatile High-Throughput Cloning...Kuhn BT, Bräuer P, Newstead S, Dawson RJP, Geertsma ER, Seeger MA (2020) Generation of synthetic nanobodies...Hetemann L, Gera J, Gmür S, Spies P, Gygax D, Geertsma ER, Dawson RJ, Seeger MA (2018) Synthetic single domain...to, like ones that are hidden in the molecular crevices proteins on the cell surface. Figure 1: ...threonines at positions that can be randomized for creating the libraries (Zimmermann et al., 2018). Find ...protein (sybody), ribosome, and mRNA. During the screening process, displayed sybodies that bind to the biotinylated...the candidate sybodies in their coat protein to screen for binding to the target protein. The protocol...

...160857 pAAV-FLEx-ER-TurboID CMV-driven, Cre-dependent Cre-dependent expression of ER-localized TurboID...99121 pAAV-ihSyn1-DIO-tTA Cre-dependent and inducible Synapsin promoter (ihSyn) Cre-dependent expression of...Gradinaru 117383 TRE-DIO-eYFP Cre-dependent and Tetracycline-inducible Cre-dependent, Tet-inducible expression...FLEX-tdTomato-T2A-SypEGFP-WPRE Syn-driven, Cre-dependent Cre-dependent expression of cytoplasmic tdTomato...FLEx mGFP-2A-Synaptophysin-mRuby Syn-driven, Cre-dependent Cre-dependent expression of membrane-bound GFP...45580 pAAV-flex-taCasp3-TEVp EF1a-driven, Cre-dependent Cre-dependent, bicistronic expression of designer...Wells 124364 pAAV-FLEX-DTR-GFP CBA-driven, Cre-dependent Cre-dependent expression of diphtheria toxin receptor...

...control without modification - either Cre is there or it’s not. Cre-mediated possibilities for site specific...control FLP and Cre expression have been developed. Read on to learn more about FLP-FRT, Cre-lox, and how...the control of Cre recombinase are used in combination with mouse lines that express Cre only in the cell...estrogen receptor (ER) ligand binding domain (LDB) to the C-terminus of FLP or Cre (pCAG-CreERT2 #14797). These...Tamoxifen inducible Cre versions can be used in these experiments. This elegant combination of Cre, FLP, and inducible...about Cre-Lox Check out Retrograde AAVs for Neurscience Research Resources on Addgene.org Find Cre-Lox.... Tamoxifen then binds to the ER-LDB domains of the fusions allowing Cre/FLP translocate to the nucleus...

...HRB (1987) A C-terminal signal prevents secretion of luminal ER proteins. Cell 48:899–907. https://doi....S, Pelham HRB (1986) An hsp70-like protein in the ER: Identity with the 78 kd glucose-regulated protein...

...and cytosolic RCaMP1h calcium sensors, ER-targeted CEPIA1er ER-mitochondria tethering by PDZD8 regulates...2023.07.18.549527 Eric Schreiter Calcium GCEPIA1-SNAP ER for sensing ER calcium A genetically encoded ratiometric ...expression of GCaMP6m/s in cells depdent on Cre, Flp, or Vcre Comprehensive Dual- and Triple-Feature Intersectional...Genetically Encoded Calcium Indicators (Constitutive or Cre-dependent) jGCaMP8 Fast Genetically Encoded Calcium...High-performance GFP-based calcium indicators (Constitutive or Cre-dependent) High-performance calcium sensors for imaging...sensors for imaging neural activity (Constitutive or Cre-dependent) Ultrasensitive fluorescent proteins for...calcium sensor jYCaMP1 variants (Constitutive or Cre-dependent) jYCaMP: an optimized calcium indicator...

...reticulum ER retention signal E2-Crimson Benjamin Glick 36204 pmTurquoise2-ER Endoplasmic reticulum ER retention...Gadella 137804 ER-mNeonGreen Endoplasmic reticulum KDEL mNeonGreen Dorus Gadella 137805 ER-mScarletI Endoplasmic...Endoplasmic reticulum ER retention signal moxGFP Erik Snapp 68126 ERoxBFP Endoplasmic reticulum ER retention signal...Erik Snapp 49150 BFP-KDEL Endoplasmic reticulum BiP ER signal sequence, KDEL TagBFP Gia Voeltz 61807 Rtn4a-GFP...St-Pierre 189772 pER-mScarlet3_N1 Endoplasmic reticulum ER retention signal mScarlet3 Dorus Gadella 61170 pCMU-ERr...Collection . Return to top Membrane Trafficking The secretory pathway is responsible for delivering proteins...mNeonGreen Dorus Gadella 212022 pcDNA3/td5StayGold(c4)=GianCreg Golgi apparatus Giantin td5StayGold Atsushi Miyawaki...

...the nucleus, mitochondria, endoplasmic reticulum (ER), vacuole or cellular membranes. Examples include... biosensor creation, and custom FRET biosensors can be created using the cpFRET kit created by Olivier...Robert Campbell’s lab display calcium-dependent increases in fluorescence of up to 11,000-fold. FRET biosensors...biosensors. Blue flame “cameleon” calcium sensors created by Roger Tsien’s lab contain two fluorescent proteins...calcium binds, it triggers CaM/M13 interaction that increases FRET transfer between the fluorescent proteins...

... Figure 2: Two-color super-resolution imaging of ER and mitochondria reveals dynamic organelle interactions...interactions. a) Labeling scheme. b) COS-7 cell ER labeled with Halo-Sec61β with SiR-CA (magenta) and outer...no wonder that self-labeling protein tags are increasingly popular. Embrace the flexibility and switch ...

...Structure Plasmids: ER mCherry-Sec61 beta BFP-Sec61 beta BFP-KDEL Rtn4a-GFP (tubular ER) Microtubules ... their synthesis in the ER to their arrival in their target compartment (e.g. the plasma membrane, the...stably localized in the donor compartment (e.g. the ER) and a reporter protein of interest (Figure A). Franck...and interactions between the endoplasmic reticulum (ER), mitochondria, cytoskeleton, and endosomes. These...upon these findings by screening for additional activation domains that increase the expression of target...Synchronization of secretory protein traffic in mammalian cells The journey of secretory proteins, from their... mutagenized and screened for speed and voltage sensitivity. This hierarchical screen led to the generation...

.... In tamoxifen-inducible Cre (Cre-ER) systems, Cre is fused to the ligand binding domain of estrogen receptor...the Esr1 domain that blocks Cre expression. Then, Cre is expressed and Cre-mediated recombination of floxed...that regulates Cre expression. The most common drug used to induce the expression of Cre is tamoxifen. ...doxycycline mediated activation of Cre to either induce or repress Cre expression using TetON or TetOFF ...using Cre-lox Read our genome engineering blog posts Resources on Addgene.org Find more about Cre-lox ... vector, creating the floxed gene segment. Crossing of mice containing a floxed gene to a Cre-expressing...mice, conditional knockout/knockin mice, use the Cre-lox recombination system to express the transgene...

...in combination with Cre-dependent transgenic mouse lines or co-infection with Cre dependent viral vectors...promoter after Cre-dependent recombination. Ofer Yizhar 198516-AAV5 pAAV_EF1a-DIO-PdCO-mScarlet-ER-miniWPRE ...PHPeB CN1851-rAAV-hI56i-minBglobin-iCre-4X2C-WPRE3-BGHpA AAV vector for Cre expression in forebrain GABAergic...Roth 83894-AAV1 pAAV-hDlx-Flex-dTomato-Fishell_7 Cre recombinase-dependent dTomato expression in forebrain...Fishell 83894-AAV2 pAAV-hDlx-Flex-dTomato-Fishell_7 Cre recombinase-dependent dTomato expression in forebrain...Fishell 83894-AAV5 pAAV-hDlx-Flex-dTomato-Fishell_7 Cre recombinase-dependent dTomato expression in forebrain...Fishell 83894-AAV9 pAAV-hDlx-Flex-dTomato-Fishell_7 Cre recombinase-dependent dTomato expression in forebrain...

...nucleus, nearly all cysteines are reduced; in the ER and Golgi apparatus and outside the cell, most cysteines...assembly tools, alongside criteria for Click Biology. Created with BioRender.com.   Principles of Click Biology...Protein (MFP) through rapid covalent coupling. Created with BioRender.com.   Table 1: A handful of...system, adds Tetracycline (Tet) transactivator to increase fluorescent protein expression C234-E25: attB2r-SNAP-attB3...Cas9 plasmids with N- or C-terminal PCV2 HUH-tag; increase homology-directed repair efficiency by linking...linking the repair template VirD2 HUH-tag For creating DNA-protein conjugates rHUH HUH-tag (RNA) Covalent...

... PHO8 is synthesized at the ER, delivered to the vacuole via the secretory pathway, and then cleaved to...In both cases, red fluorescence in the vacuole increased with longer exposure to autophagy-inducing conditions...

...expressing HTT, C9ORF72, LRRK2, Synuclein, and more. Study ER structure and function with siRNA-resistance SigmaR1...Heliyon. 2020) Use VAP expression plasmids to study ER contact with other organelles. (Mattia et al. EMBO...blood brain barrier integrity, and using CRISPR screens to understand more globally how neurons function... et al. Cell. 2021) Conduct multimodal genetic screens in human iPSC-derived neurons with a CRISPRi-based...antibodies and scFvs for neuroscience research created with high-volume hybridoma sequencing on the NeuroMabSeq...community. This collection highlights plasmids created with support from the BRAIN Initiative. Jackson...Link opens in a new window) . This resource was created with support from the Chan Zuckerberg Initiative...

...postsynaptic AMPA receptor and is endocytosed, the Cre recombinase payload activates a floxed reporter gene...synapses. In addition, ATLAS itself could be expressed Cre-dependently to allow tracing exclusively from genetically... components (nucleus, endosomes, lysosome, golgi, ER, inhibitory synapses, and cytoskeleton components... 5: Mechanism of ATLASCre. VAMP2 = synaptobrevin 2; At = ALFA-tag; BACE = β-secretase; AF = AMPA.FingR...TnpB) or a gRNA (Cas9). Drawings not to scale. Created with BioRender.com. Steven Jacobsen and Jennifer...potent retrograde labeling of projection neurons. Created with BioRender.com. Addgene has tested these...Huang, et al. (2025) under CC BY-NC-ND license, created with BioRender.com. When delivered by AAV ...

...A-induced perturbation in biological processes in ER-negative HEK293 cells." PLoS One, 2014. 9(6): p. ...making their surrounding media acidic through the secretion of lactic acid (a by-product of cellular metabolism...

...to label proteins moving to and from the nucleus, ER, mitochondria, stress granules, and more. For added...enable the creation of CAR-T cells directly within the body. Overall, DIRECTED can increase on-target ...reprogram delivery vehicles like lentiviral vectors, CreVLPs, and eVLPs for in vivo and in vitro applications.... The Ting lab recently made two deposits that increase the utility of this technique.  By engineering... O’Neill The Cohen lab and colleagues recently created a system for recording the transcriptional history...resembling a “ticker tape” recording. The authors created externally-timed bands of color with HaloTag-iPAK4...thin sections of tissue, the Wu Lab is working to create a three-dimensional atlas of protein expression...