Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 15 results for: KAS III;

Showing: 1 - 15 of 15 results

  1. Expanding the Targeting Scope and Editing Efficiency of Adenine Base Editors

    Type
    Blog Post
    ...plasmid in the host bacterium that expresses gene III. Gene III is under the control of a T7 RNA polymerase ...bacteriophage, which rely on the expression of gene III to create infectious particles. A plasmid-based genetic...circuit in the bacteria links the expression of gene III to base editor activity and editing speed and consists...active variant of the base editor, a functional gene III product is produced and can infect bacterial cells...circuit in the bacteria links the expression of gene III to base editor activity and editing speed. Created...catalytically impaired Cas protein (dCas or Cas nickase) fused to a DNA-modifying enzyme, in this case ...

  2. New CRISPR Tools: Cas7-11 and PASTE

    Type
    Blog Post
    ... to have derived from the more complex type III-A and III-B systems. Though it was composed of many protein...ancestors. They classified the system as Class I, Type III-E, and then used this information to identify a large...Cian Schmitt-Ulms, Rohan N. Krajeski, Justin Lim, Lukas Villiger, Wenyuan Zhou, Kaiyi Jiang, Nathaniel Roberts... Roberts, Liyang Zhang, Christopher A. Vakulskas, John A. Walker II, Anastasia P. Kadina, Adrianna E. ...

  3. CRISPR-mediated Plant Base Editors

    Type
    Blog Post
    ...single guide RNA (protospacer+scaffold) in a pol-III (OsU3) promoter driven expression system. We use ...editors by tethering cytosine deaminase with Cas9 nickase (nCas9). nCas9 creates a nick in the DNA by cutting...of the base editors mentioned above are SpCas9 nickase-based and depend on the presence of an ‘NGG’ PAM...PAM sequences other than NGG (Table-1). Type Nickase Cas9 (D10A) Deaminase PAM requirements Reference...

  4. CRISPR History and Development for Genome Engineering

    Type
    Collection
    ...palindromic repeat. The tracrRNA helps recruit RNAse III and Cas9 enzymes, which together separate the individual...multi-domain effector) Type I (Cas3) Type II (Cas9) Type III (Cas10) Type V (Cpf1) Type IV (Csf1) Type VI (Cas13... editing rates. Advances in this area include: Nickase mutant: cuts only one strand of DNA and can be ...

  5. TALEN Plasmids and Kits

    Type
    Collection
    ...lacZ fragment for blue/white-screening in E.coli , (iii) CAG promoter and Kozak sequence to drive efficient... by Golden Gate. Validated in several species. Takashi Yamamoto Additional Plasmids for Use with Addgene...TALEN Construction and Evaluation Accessory Pack Takashi Yamamoto This accessory pack contains modified ...

  6. Immunology Research Plasmids and Resources

    Type
    Collection
    ...-C motif) ligand 7) B-TG1, Beta-TG, CTAP-III, CTAP3, CTAPIII, CXCL7, LA-PF4, LDGF, MDGF, NAP-2, PBP, SCYB7... (semaphorin) 3A Hsema-I, Hsema-III, MGC133243, SEMA1, SEMAD, SEMAIII, SEMAL, SemD, coll-1 SEMA3B sema...signaling proteins consists of Type I, Type II, and Type III interferons. They trigger the defensive responses..., SER2 KDR kinase insert domain receptor (a type III receptor tyrosine kinase) CD309, FLK1, VEGFR, VEGFR2...superfamily, member 3) CD18, D12S370, LT-BETA-R, TNF-R-III, TNFCR, TNFR-RP, TNFR2-RP, TNFRSF3 MC1R melanocortin...

  7. CRISPR 101: Multiplex Expression of gRNAs

    Type
    Blog Post
    ...reasons to multiplex gRNAs, some being: Using dual nickases to generate a knockout or edit. This can help ...variants are available: wt humanized Cas9, D10A nickase mutant (Cas9n), dCas9 (transcriptional repression...Comparison of Multiplex Strategies including Standard PolIII-gRNA cassette, Csy4-cleavable cassette, and PTG...

  8. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ...Redox-sensitive cytosolic protein sensor Mitochondrial complex III is required for hypoxia-induced ROS production and...Methods. 2020 May 25. pii: 10.1038/s41592-020-0835-7. Kaspar Podgorski Calcium HaloCaMP1 chemigenetic calcium...Biol. 2021 Jun 29. pii: S2451-9456(21)00301-9. Takashi Tsuboi Glucose iGlucoSnFR-TS biosensor for intracellular...cell imaging. Sci Rep. 2020 Nov 11;10(1):19562. Takashi Tsuboi Maltose Single fluorescent protein biosensor...cell imaging. Sci Rep. 2020 Nov 11;10(1):19562. Takashi Tsuboi Sucrose FRET-based sensor to monitor sucrose...synaptic transmission. Nat Methods. 2023 May 4. Kaspar Podgorski Glutamate FRET sensor to monitor glutamate...

  10. 22 Hot Plasmid Technologies from 2014

    Type
    Blog Post
    ...targeting sequence of cytochrome c oxidase subunit VIII, so that they can be imported to the mitochondrial...TALEN assembly and activity? The laboratory of Takashi Yamamoto has created a complete TALEN assembly ...

  11. CRISPR Plasmids - Prime Edit

    Type
    Collection
    ...BsaI + HindIII No Caixia Gao 172716 pPEgRNA Bacteria J23119 (BBa_J23119) pegRNA SpeI + HindIII No Tilmann... (RT) is fused to the C terminus of Cas9 H840A nickase. The fusion enzyme is capable of installing targeted...to a specific genomic DNA locus and directs the nickase to the target site. The longer pegRNA also encodes...During prime editing, the pegRNA directs the Cas9 nickase to the target sequence where it nicks the non-target...

  12. CRISPR Plasmids - Empty gRNA Vectors

    Type
    Collection
    ...Marraffini pgRNA-bacteria 44251 Bacteria SpeI + HindIII none S. pyogenes Qi pDD162 (Peft-3::Cas9 + Empty..., Wildonger gRNA_Cloning Vector 41824 Mammalian AfIII none S. pyogenes Bleocin Church MLM3636 43860 Mammalian...pyogenes Joung sgRNA with U6 promoter 48962 Worm HindIII none S. pyogenes de Bono sgRNA with rpr-1 promoter...Qi pRPR1(1xTetO)_gRNA_handle_RPR1t 62966 Yeast HindIII none S. pyogenes Puro Lu pML104 67638 Yeast BclI-SwaI...Zhang AAV:ITR-U6-sgRNA(backbone)-hSyn-Cre-2A-EGFP-KASH-WPRE-shortPA-ITR 60231 Mammalian/AAV SapI none S...pyogenes Yang pRPR1_gRNA_handle_RPR1t 49014 Yeast HindIII none S. pyogenes LEU2 Lu pDestTol2CG2-U6:gRNA 63156...guide RNA expression cassettes and a Cas9 nuclease/nickase expression cassette using the Golden Gate cloning...

  13. CRISPR Plasmids - Bacteria

    Type
    Collection
    ...Plasmid Promoter PI Publication Nick CRISPR/Cas nickase mutants introduce gRNA-targeted single-strand breaks...breaks created by wild type Cas enzymes. To use a nickase mutant, you will need two gRNAs that target opposite...strategies reduce unwanted off-target effects. Nickase mutants can also be used with a repair template... Promoter PI Publication Prime Edit Cas9 H840A nickase fused to a reverse transcriptase (RT) is capable...editing guide RNA (pegRNA). The pegRNA directs the nickase to the target site by homology to a genomic DNA...Marraffini 44251 pgRNA-bacteria BBa_J23119 SpeI + HindIII Ampicillin none, need Cas9 plasmid Qi Do you have...

  14. CRISPR Plasmids - Yeast

    Type
    Collection
    ...Selectable Marker PI Publication Nick CRISPR/Cas nickase mutants introduce gRNA-targeted single-strand breaks...breaks created by wild type Cas enzymes. To use a nickase mutant, you will need two gRNAs that target opposite...strategies reduce unwanted off-target effects. Nickase mutants can also be used with a repair template...PAM = NGG) 49014 pRPR1_gRNA_ handle_RPR1t pRPR1 HindIII S. cerevisiae LEU2 none, need Cas9 plasmid Lu Do...

  15. Neurodegeneration Plasmid Collection

    Type
    Collection
    ... ataxia 6 Annette Dolphin 206115 Cav2.1 HA EI,II,III,IVA (E320A, E670A, E1411A, E1707A) pCAGGS CACNA1A... ataxia 6 Annette Dolphin 206121 Cav2.1 HA EI,II,III,IVA (E320A, E670A, E1411A, E1707A) pcDNA3 CACNA1A...telangiectasia Michael Kastan 32300 hATMS1981A ATM His, Flag CMV Ataxia telangiectasia Michael Kastan 32813 CMV-hEAAT1... Slc1a3 nickase 1 SLC1A3 HA U6, Cbh Episodic ataxia Walker Jackson 129411 px335 Slc1a3 nickase 2 SLC1A3...ATM His, Flag CMV Ataxia telangiectasia Michael Kastan 31986 pcDNA3.1(+) Flag-His-ATM kd ATM His, Flag...
Showing: 1 - 15 of 15 results