... Adeno-associated virus (AAV) is a small, single-stranded DNA parvovirus. AAV can infect humans and some... generally not as long lasting as AAV (up to several weeks vs. AAV’s several years). But these viruses...Adeno-associated Virus (AAV) Guide Addgene’s viral plasmids and resources Addgene’s viral services: AAV Resources...currently known to cause any diseases. Recombinant AAV largely does not integrate, and the addition of a...replicate and divide along with the endogenous DNA. AAV also has low immunogenicity, meaning it does not ...conducive to targeting all cell types. Altogether, AAV has many excellent features for human gene therapy...and is currently being used in clinical settings. AAV is also a cool tool in the laboratory! It is ideal...

...multiple AAV variables.    Serotypes Serotype refers to the different protein compositions of AAV capsids...when designing and testing your AAV.  Figure 2: Comparison of AAV serotypes 1, 2, 5, 6, 8, and 9...Alternate Route for Adeno-associated Virus (AAV) Entry Independent of AAV Receptor. Journal of Virology, 92(7)...Viral Vectors 101: An Introduction to AAV Viral Vectors 101: The AAV Retrograde Serotype Viral Vectors 101...Using AAV vectors in complex biological systems can be tricky at best, and downright infuriating at worst... in and start injecting your virus, a successful AAV experiment starts with validation and optimization...usually faster and easier to do the validation first.  AAV-mediated gene expression can differ extensively depending...

... and quality control are described below. AAV Production AAV distributed by Addgene has been produced ...purity of the AAV preparation. Figure 1: Silver staining of purified and non-purified AAV subjected...arrowhead). Scale bar = 100 nm.   AAV resources Browse AAV preps available from Addgene. After receiving...Addgene blog Find and share AAV Data with Addgene's Data Hub Digital Droplet for AAV Quantification Viral Vectors...Figure 2: AAV Pro cells were transduced with either pAAV-Ef1a-mCherry-IRES-Cre (55634-AAVrg) alone at ...84445-AAVrg).   Electron Microscopy The ratio of empty to full (i.e., genome-containing) AAV particles..., we’ll walk you through how we produce both our AAV preps and our lentiviral preps.   Viruses are generated...

...Alternate Route for Adeno-associated Virus (AAV) Entry Independent of AAV Receptor. J Virol 92. https://doi.org...Considerations When Using AAVs Three Key Considerations For Precise Neuronal Targeting Using AAV Technologies Resources... on Addgene.org Find ready to use AAV vectors at Addgene. Find AAV plasmids to use in your experiments...of viruses used (100798-AAV, lot #v15287, titer 1.7x10e13 GC/mL and 100799-AAV1, lot # v14715, titer 2.4x10e13...their cells of interest and recombinase-dependent AAV vectors for selective transgene expression. While... or spurious expression of recombinase-dependent AAV vectors. In this article, we will discuss some of...Fischer, KB. 2019). This means that all DIO/FLEx AAV vectors will have some level of recombination that...

...operator. AAV AAVs are the workhorses of both chemo- and optogenetics. We’re therefore beginning our AAV viral...currently available) and adeno associated virus (AAV, primarily chemogenetics tools for now but with optogenetic...researcher Connie Cepko has been helping us with AAV testing and recently had this to say about the service...preparations to the scientific community. Addgene's AAV preparations have worked well in my lab, including...ll provide a brief rundown of what lentiviral and AAV preps are available below, but you can always head... will be available soon. We’re also distributing AAV preps with particular serotypes that can be used ...You can use these as controls to determine if your AAVs are getting to the right cells using your particular...

...YouTube page! AAV purification by iodixanol gradient ultracentrifugation This video goes over AAV vector purification...  Check out the AAV Purification protocol page for more detailed instructions. AAV titration by qPCR...service in 2016, Addgene has shipped thousands of AAV and lentiviral preps to scientists around the world...strategy to confirm the identity and purity of all AAV viral lots. Aligning with Addgene’s mission to “...your qPCR to determine the genome copies in a given AAV preparation. For detailed instructions and calculations...stock used to generate a standard curve, visit the AAV Titration by qPCR written protocol page. Getting...

...also offered as AAV1 (along with AAV PHP.eB, AAV Retrograde, AAV2, AAV5, AAV8, and AAV9). Once activated... the Dolcetto lentiviral prep here Control AAV Control AAV allow researchers to not only interpret their...offer control AAV in smaller aliquots of 20ul for serotype testing to help you pick the best AAV for your ...Testing AAV inventory for the entire collection! Plasmid Serotype Name Depositor 27056 AAVrg pAAV-Ef1a-DIO...ready-to-use viral vectors. Here are some of the AAV and lentiviral preps we have released in the last...confirm expression in target cells. While the various AAV serotypes can be used to achieve different patterns...specific manipulation of neurons. We have expanded our AAV collection to include two new ChR2 constructs that...

...powerful tool for quantifying AAV for some time now. When titrating AAV by qPCR, viral DNA is amplified...Since Addgene began producing AAV vector preparations, we have used quantitative PCR to evaluate the ... is where droplet digital PCR (ddPCR) comes in. AAV titering using quantitative PCR Before we dive into...reactions with known quantities of DNA. Additionally, an AAV reference, a virus with a known titer, can be used...and both results would be valid. This is why the AAV reference is critical. It allows you to determine...reference, meaning saving on reagents (and time)! AAV titering using droplet digital PCR Droplet digital... for your PCR experiment. The process to titrate AAV by ddPCR begins with diluting the virus. It is important...

...Addgene’s first AAV Data Hub Challenge, now taking place Dec. 6, 2022! Share your AAV data, connect with...with other researchers working with AAV, support open science, and maybe even win a $500 travel prize ...can submit data from their CLOVER lab or Addgene AAV experiments to the Data Hub. Submit your data by ... competition, where you can get all your burning AAV questions answered.  Interested? You can learn more...

...improve their AAV yields. Given the wide range of downstream applications scientists rely on our AAV preparations...membrane When packaging adeno-associated viral vectors (AAV) not all serotypes are equal; while some serotypes...To our knowledge the effect of hyperosmolarity on AAV production yields had yet to be examined. Increased...sorbitol. As sorbitol is already a common excipient in AAV formulation buffers, this sugar may be a more attractive...fluorescent dye and transduction of GFP-expressing AAV particles we did not observe any difference between...alike as an easy and inexpensive method to improve AAV yields. Although we only investigated 3 serotypes... approach will be beneficial for a host of other AAV serotypes; it will be exciting to test this approach...

...in the lab Over-Agar Antibiotic Plating Protocol AAV Purification Purify adeno-associated virus from a... a preparation produced in mammalian cells AAV Purification by Iodixanol Gradient Ultracentrifugation ...workspace, and maintaining sterility while working. AAV Titration by qPCR Use qPCR to measure the physical...titer of your adeno-associated virus preparation. AAV Titration by qPCR Using SYBR Green Technology Protocol...

...single gene knockout. Top AAV: pAAV-hSyn-DIO-hM4D(Gi)-mCherry Our current AAV inventory consists almost...of the human synapsin promoter. Purpose of this AAV pAAV-hSyn-DIO-hM4D(Gi)-mCherry can be used to induce...ready-to-use lentivirus and adeno-associated virus (AAV) to scientists around the world. We began with only...expanded our viral inventory to 25 lentiviruses and 25 AAVs. These viruses have been distributed in over 200...again) to find the most requested lentivirus and AAV of 2016. The top viruses of 2016 were (drumroll please...transduction efficiency, tropism and axonal transport of AAV serotypes 1, 2, 5, 6, 8 and 9 in the mouse brain....Blog Learn More About Lentivirus Learn more About AAV Get the Scoop on Our Viral Service Resources on ...

...week, we launched our new AAV Data Hub, an open platform for scientists using AAV to find and contribute ... posts Learn more about AAVs Resources on the Addgene website Visit the AAV Data Hub Learn more about...about the viral service Read our AAV guide ...last year on sucrose supplementation to increase AAV2 yields and we’re eager to share more! Lastly, we...

...cell lines from a polyclonal pool of stable cells AAV Production in HEK293 Cells Produce adeno-associated...deliver your gene of to the target of your choice AAV Purification by Iodixanol Gradient Ultracentrifugation...preparation produced in mammalian cells Watch the Video! AAV Titration by qPCR Using SYBR Green Technology Use...adeno-associated virus preparation Watch the Video! AAV Titration by ddPCR Use ddPCR to measure the physical...

...about Addgene's 2018 AAV Education and Development (AAVED) workshop series....Development (AAVED) Series AAV Technologies and Methods for Targeted Neuronal Manipulation Meeting Date September... The AAV Education and Development...September 21, 2018 Contact Us Questions? Contact us at AAVED@addgene.org Sponsor We are grateful to The Kavli...brainstorm practical strategies for using combinations of AAV-based molecular tools to achieve targeted neuronal...Fan 9:30 AM Cre and Flpo Katherine Matho 10:15 AM AAV Retrograde Tracing Gowan Tervo and Sarada Viswanathan...Promoter/Enhancer Specificity, Serotype specificity, AAV dosing, and Other Forms of Regulation Ben Deverman...

...concentrations or AAV titer for the best results. The timing of sacrifice when using Tetbow AAVs - You will ... #104105) using in utero electroporation or with AAVs (prepared with Addgene #104110 - #104112) (Figure...sacrifice the animals and fix the samples. When you use AAVs, it typically takes a few weeks to achieve optimal...your Tetbow experiment Plasmid concentrations and AAV titer - The XFP genes have to be introduced at ~2...achieve an adequate expression level of the Tetbow AAVs. However, you should not wait too long, as too much...Addgene blog Read about Brainbow Learn about using AAVs for neuronal tracing Visit the viral vectors blog...service See our fluorescent protein collection Find AAVs at Addgene ...

...interest. AAV or lentivirus?  Both AAV and lentiviral vectors are used for intracerebral injections. AAV particles...can hold up to 8 kb, while AAV can only carry around 4.5 kb. Different AAV serotypes have preferences ...stereotaxic injection. Find more details about choosing AAV or lentiviral vectors in our previous blog posts....enough to maximize possible outcomes. Typically, AAVs have to be minimum around 1012-1013 vg/ml (vector...viral vectors blog posts Find tips for preparing AAVs View our Beginner's Guide to Viral Vectors Resources...viral service Find viral protocols and videos Find AAVs for your research ...cell types. For example, AAV1 and AAV9 serotypes have strong neuronal tropism, AAV5 targets both neurons ...

...likely someway you can apply it in neuroscience. AAV tools from Addgene are already having an impact on...not familiar, ready-to-use adeno associated virus (AAV) and lentivirus are now available from Addgene. While...Neuroscientists are particularly interested in using AAVs because they provide a highly versatile means of...different cell types with minimal immunological effects. AAVs are used in experiments targeting genes to specific.... This helped reinforce our goals of making more AAVs and educational content to help researchers use ...into the field much easier! If there’s a plasmid or AAV tool that you can’t find, you can always suggest ... plasmid to us, request that we start making the AAV, or help us produce new educational content on the...

...immunogenicity, and the long-term expression of AAV delivered genes. AAV serotypes have been extensively studied...neuronal tracing Retrograde AAV: Making the journey from axon to nucleus AAVs CREATEd for gene delivery ...Monster probes at Addgene Browse all AAV vectors Find ready-to-use AAV preps ...three decades, recombinant Adeno-associated virus (AAV) has been widely used to deliver genetic tools to...atlas enables researchers to quickly find the best AAV serotypes to target the neurons they’re studying....how neurons connect to each other). Combined with AAVs, FPs can be easily expressed in the brain and can...structures or low-abundance proteins. When delivered with AAV, these probes can be used to label neuronal circuits...

...transient change in pH affects AAV function. Furthermore, another study tested AAV infectivity after a range...recommend that once you receive your AAV from Addgene, you store the AAV at -80°C for 96 hours prior to thaw...to store lentivirus and the ones we use to store AAV. Our results showed that, once thawed, there was ...from 7.4 to 5.0) in the pH of samples stored in our AAV tubes (Fig. 1). It’s important to note that the change...range of pH exposures. They showed that AAV infectivity was not altered by a short 2-hour exposure to pH ... aav...