...fusion tag based on the human DNA repair protein AGT that gets covalently linked to derivatives of its...Also in 2008, Kai Johnsson’s lab reported another AGT-based tag named CLIP-tag, which reacts with O2-benzylcytosine...benzylguanine (BG) (Keppler et al., 2003). This engineered hAGT later became known as SNAP-tag. There is also a ...

...Forward Primer will use the sequence 5'-ATGTGGCATATCTCGAAGTAC-3' for the region that binds the ORF and...primer, making our Forward Primer 5'-GAATTCATGTGGCATATCTCGAAGTAC-3'. Many restriction enzymes do not cut...final Forward Primer sequence of 5'-TAAGCAGAATTCATGTGGCATATCTCGAAGTAC-3'. For the Reverse Primer, the design...of the ORF, including the stop codon (5'-TGGCATATCTCGAAGTACTGA-3'), then adding NotI (GCGGCCGC) and then.... This gives us a sequence of 5'-TGGCATATCTCGAAGTACTGAGCGGCCGCTAAGCA-3' (30bp with 18bp of homology to...chose for our reverse primer (5’-TGGCATATCTCGAAGTACTGAGCGGCCGCTAAGCA-3’) into this calculator we get a...final Reverse Primer sequence of 5’-TGCTTAGCGGCCGCTCAGTACTTCGAGATATGCCA-3’. Preparing the PCR product for...

...canonical loxP sequence is ATAACTTCGTATA-GCATACAT-TATACGAAGTTAT. The loxP sequence does not occur naturally ...

... asun TAL3404 & TAL3405 TAACTCAAGCCAACTCCAggaaacatcaggagtttGCAGGAAGATTGAGTTCA atf6 TAL3406 & TAL3407 TCCAACCAGCAAAAAGACccatccaggtgtgccccaAGGTTTCCATTCAGCCTA...TCCAACCAGCAAAAAGACccatccaggtgtgccccaAGGTTTCCATTCAGCCTA atg5 TAL3218 & TAL3219 TAAAGTCAAGAAACATTTtctcaaagtcatgaagGCAGAGGATGTGGAGGAA atg12 TAL3216 & TAL3217 ...TAL3217 TTAATCAGTCGTTTGCGCcatcaccggaccaagaAGTTGGTGTGCTTTTTGA avpl TAL3220 & TAL3221 TGCTGTGTGTGCTCGCGCtctccacgctctcgtcTGCCTGCTACATCCAGAA...TCAGCTCCCCGGAGAGGCaccgcactgctccagggTTGGATGATGGACATGGA bsx TAL3034 & TAL3035 TCTCAGACTCACAGTTATccggactcgagaaaagGTTCGAGATCCAGAGATA btr30 TAL3036 & TAL3037 ...TATAACAAATCCCACCACattgcccgatatcttgAATACTTCCAAAACAAAA Ccl2 TAL3222 & TAL3223 TTTTCGTTTTCCACTATAaagatcccagttaagcAGGTTCAGAGTTATCATA cebpa TAL3040 & TAL3041 ... cebpb TAL3224 & TAL3225 TGGAAGTGGCCGGTTTTTacgagggggactacctCGCTTTCCACAGCACTAA cebpd TAL3042 & TAL3043 ...TAL3043 TGACTTCTGCTGCGTCCAtgtctgacatgtacaatCTGGACTCGCAGTGCGTA CHCHD4 TAL3408 & TAL3409 TCTACCTAATGGCGACATaaactggaactgcccgtGTTTGGGCGGCATGGCCA...

...sapiens GACTGTAAGTGAATTACTT 69539 cut S. pyogenes 26130722 Yan HPRT1 H. sapiens CTGATAAAATCTACAGTCAT 58778...synthetic GCGTATAGTACACATTATACG 66585 cut S. pyogenes 26018130 Xue LRTM2 H. sapiens GCTGGCGGAAGACAGAGTGC 69237...musculus GCTGTAGTAATATCTGCTAT 66588 cut S. pyogenes 26018130 Xue Pten M. musculus GTTTCATAGCGGCCACGAAGT 66587...elegans GGAAGGACATAGTTGTCAT 59935 cut S. pyogenes 25161212 Fire sqt-1 C. elegans TGTGGAGTTGGGGTAGCGT 60212...sapiens GGTGAGTGAGTGTGTGCGTG 69235 cut S. pyogenes 26480473 Wolfe TS4 H. sapiens GAGTCCGAGCAGAAGAAGAA 69236...elegans ATATCAGTCTGTTTCGTAA 47550 cut S. pyogenes 23995389 Goldstein tyr D. rerio CCCCAGAAGTCCTCCAGTCC 46761...sapiens GGGTGGGGGGAGTTTGCTCC 47505 cut S. pyogenes 23792628 Joung VEGF H. sapiens GGTGAGTGAGTGTGTGCGTG 47507...

...) ABL1 TAL2380 & TAL2381 TACCTATTATTACTTTATggggcagcagcctggaaAAGTACTTGGGGACCAA AKT2 TAL2382 & TAL2383 TGTGTCTTGGGATGAGTGggtcagtgttctggtgCTCACAGGATGGCTGGCA... TGTGTCTTGGGATGAGTGggtcagtgttctggtgCTCACAGGATGGCTGGCA ALK TAL2292 & TAL2293 TCCTGTGGCTCCTGCCGCtgctgctttccacggcAGCTGTGGGCTCCGGGA... AXIN2 TAL2200 & TAL2201 TCCCTCACCATGAGTAGCgctatgttggtgacttGCCTCCCGGACCCCAGCA BAX TAL2296 & TAL2297 TGTGCGATCTCCAAGCACtgaggggcagaaactcCCGGATCGGGCGCTGCCA...TGTGCGATCTCCAAGCACtgaggggcagaaactcCCGGATCGGGCGCTGCCA BCL6 TAL2412 & TAL2413 TTTTCAAGTGAAGACAAAatggcctcgccggctgACAGCTGTATCCAGTTCA BMPR1A TAL2260 & TAL2261... BRCA2 TAL2302 & TAL2303 TTAGACTTAGGTAAGTAAtgcaatatggtagactGGGGAGAACTACAAACTA CBX3 TAL2358 & TAL2359 TCTGCAATAAAAAATGGCctccaacaaaactacaTTGGTAAGTTAATGAAAA... TCTGCAATAAAAAATGGCctccaacaaaactacaTTGGTAAGTTAATGAAAA CBX8 TAL2360 & TAL2361 TGGAGCTTTCAGCGGTGGgggagcgggtgttcgcgGCCGAAGCCCTCCTGAA...CCND1 TAL2304 & TAL2473 TGGAACACCAGCTCCTGTgctgcgaagtggaaaccatCCGCCGCGCGTACCCCGA CDC73 TAL2202 & TAL2203...

...-TALEN-14-Right TAL2027 TGAAGAAGTCGTG eGFP-TALEN-15-Left TAL2028 TCTTCAAGTCCGC eGFP-TALEN-15-Right TAL2029...eGFP-TALEN-30-Right TAL2059 TGAAGTTCACCTTGAT eGFP-TALEN-31-Left TAL2060 TACGGCGTGCAGTGCTT eGFP-TALEN-31-Right ...eGFP-TALEN-38-Left TAL2098 TGCAGTGCTTCAGCCGCT eGFP-TALEN-38-Right TAL2099 TTGAAGAAGTCGTGCTGC eGFP-TALEN-39-Left...48-Left TAL2084 TGAAGTTCGAGGGCGACACCC eGFP-TALEN-48-Right TAL2085 TTGAAGTCGATGCCCTTCAGC...TAL2005 TGGGCCAGGG eGFP-TALEN-04-Left TAL2006 TGGAGTACAA eGFP-TALEN-04-Right TAL2007 TGATATAGAC eGFP-TALEN... TTCAGCCGCTACCC eGFP-TALEN-20-Right TAL2039 TTGAAGAAGTCGTG eGFP-TALEN-21-Left TAL2040 TACAACAGCCACAA eGFP-TALEN...TTCAAGGAGGACGGCAA eGFP-TALEN-34-Right TAL2091 TTGTAGTTGTACTCCAG eGFP-TALEN-35-Left TAL2092 TACAACTACAACAGCCA...

...CODA CODA aGTCCTCCGATGGCCCAGGTGATGTTc SDC3 OZ595 and OZ596 CODA CODA aGTCCGCCTCCACCCGAGGTAGAG zgc:111821...gCGTGACCTCgctgatAAGAAGAGg xylt1 OZ509 and OZ510 OPEN OPEN cACCCTCTACaggacaGTAGCGGGTg zgc:153086 OZ511 and OZ512 OPEN OPEN ...aGACTCCGTCccgtcgtGCTGAGGGCc hnf1a OZ521 and OZ522 OPEN OPEN aACCTTCCCCagagaGAGGTAGTGg VDAC2 OZ523 and OZ524 OPEN OPEN gCTCCCAACCactttGCTGGTGTCa...lipoprotein receptor) OZ539 and OZ540 OPEN OPEN tTGCATCCACggcagTCGGCAGTGc Encephalopsin (Opn3) OZ541 and OZ542 OPEN...aTCCATCCCAaacacatGTGGCAGAAt NFATc1 OZ543 and OZ544 OPEN OPEN aCCTCGCCTCagtgtGACGGAGGAc Swachmann-Bodian-Diamond Syndrome Gene...tTACTTCTGCaatgatGTGGTGGCTc vipr1 OZ559 and OZ560 OPEN OPEN gGCCATCAGCtcgcattGGAGAAGTAg NRF2b (nfe212b) OZ561 and OZ562 OPEN ...tGTCTTCATCTCCTTCAGATGCTGTCc fam82a2 OZ573 and OZ574 CODA CODA gCACCGCTGCAGTAAGAGCTGAGGAAt morc3b (NP_001003579.1) OZ575 and OZ576...

...Mammalian U6-driven sgRNA (NMm1) targeting GTCCCCTCCACCCCACAGTG 48675 M-ST1n-VP64 Mammalian ST1-VP64 nuclease-null...Mammalian U6-driven sgRNA (STm1) targeting GTCCCCTCCACCCCACAGTG 48674 M-SPn-VP64 Mammalian SP-VP64 nuclease-null...expression: targets SP to protospacer B (ACTTTAAAAGTATTCGCCAT), p15A/chloramphenicol 48651 PM-NM!TA Bacterial...expression: targets NM to protospacer B (ACTTTAAAAGTATTCGCCAT), p15A/chloramphenicol 48653 PM-ST1!TA Bacterial...expression: targets ST1 to protospacer B (ACTTTAAAAGTATTCGCCAT), p15A/chloramphenicol 48655 PM-TD!TA Bacterial...expression: targets TD to protospacer B (ACTTTAAAAGTATTCGCCAT), p15A/chloramphenicol 48657 DS-SPcasN- ...Mammalian U6-driven sgRNA (SPm) targeting GTCCCCTCCACCCCACAGTG 48677 M-tdTom-SP Mammalian tdTomato activation...

...) Prefix Enzymes: EcoR1 , NotI , XbaI Suffix: T ACTAGT A GCGGCCG CTGCAG Suffix Enzymes: SpeI , NotI , ...BioBrick BB-2 (RFC 12) Prefix: GAATTC GCGGCCGC T ACTAGT G Prefix Enzymes: EcoR1 , NotI , Spel Suffix: GCTAGC...CTGCAG Suffix Enzymes: Nhel , NotI , PstI Scar: GCTAGT Features: 6 bp scar encodes Ala-Ser; Allows for...G Prefix Enzymes: EcoR1 , NotI , XbaI Suffix: T ACTAGT A GCGGCCG CTGCAG Suffix Enzymes: SpeI , NotI , ...EcoR1 , NotI , XbaI, (NgoMIV) Suffix: ACCGGT TAAT ACTAGT A GCGGCCG CTGCAG Suffix Enzymes: Agel , Spel , ...

...primer Luc-F AGTCAAGTAACAACCGCGA 3' end of luciferase, forward primer LucNrev CCTTATGCAGTTGCTCTCC 5' end of...primer M13 (-21) Forward TGTAAAACGACGGCCAGT In lacZ gene M13 (-40) GTTTTCCCAGTCACGAC In lacZ gene M13 Reverse...Human U6 promoter, forward primer LucNrev CCTTATGCAGTTGCTCTCC 5' end of luciferase, reverse primer M13 ...AUG1 promoter, forward primer AUG1 Reverse GAAGAGAAAAACATTAGTTGGC (Invitrogen) For Pichia vectors with AUG1...AUG1 promoter, reverse primer BGH Reverse TAGAAGGCACAGTCGAGG (Invitrogen) Bovine growth hormone terminator...terminator, reverse primer Ecdysone Forward CTCTGAATACTTTCAACAAGTTAC (Invitrogen) Drosophila heat shock promoter...promoter, forward primer EF-1a Forward TCAAGCCTCAGACAGTGGTTC (Invitrogen) Human elongation factor-1a promoter...

...protein homolog (mouse) AGRT, ART, ASIP2, MGC118963 AGT angiotensinogen (serpin peptidase inhibitor, clade...FLJ92595, FLJ97926, SERPINA8 AGTR1 angiotensin II receptor, type 1 AG2S, AGTR1A, AGTR1B, AT1, AT1B, AT1R, AT2R1...AT2R1, AT2R1A, AT2R1B, HAT1R AGTR2 angiotensin II receptor, type 2 AT2, ATGR2, MRX88 AMBN ameloblastin ...dJ647M16.1 APLN apelin XNPEP2 APLNR apelin receptor AGTRL1, APJ, APJR, FLJ90771, MGC45246 AR androgen receptor...

...Forward Primer will use the sequence 5'-ATGTGGCATATCTCGAAGTAC-3' for the region that binds the ORF and...primer, making our Forward Primer 5'-GAATTCATGTGGCATATCTCGAAGTAC-3'. Many restriction enzymes do not cut...final Forward Primer sequence of 5'-TAAGCAGAATTCATGTGGCATATCTCGAAGTAC-3'. For the Reverse Primer, the design...of the ORF, including the stop codon (5'-TGGCATATCTCGAAGTACTGA-3'), then adding NotI (GCGGCCGC) and then... This gives us a sequence of 5'-TGGCATATCTCGAAGTACTGAGCGGCCGCTAAGCA-3' (30bp with 18bp of homology to...chose for our reverse primer (5’-TGGCATATCTCGAAGTACTGAGCGGCCGCTAAGCA-3’) into this calculator we get a...final Reverse Primer sequence of 5’-TGCTTAGCGGCCGCTCAGTACTTCGAGATATGCCA-3’. Experimental Procedure Run PCR...

... ) fwd ITR primer, 5'-GGAACCCCTAGTGATGGAGTT rev ITR primer, 5'-CGGCCTCAGTGAGCGA ITR-containing plasmid...

... Forward Primer: 5’-CGGCCTCAGTGAGCGA ITR Reverse Primer: 5’-GGAACCCCTAGTGATGGAGTT ITR Probe: -FAM-CACTCCCTCTCTGCGCGCTCG-BBQ...

... the human O6-alkylguanine-DNA-alkyltransferase (hAGT), a DNA repair protein, and is suitable for both...

...using a U6 promoter forward primer: CGTAACTTGAAAGTATTTCGATTTCTTGGC. This is a representative sequencing...

...primers/probe targeting RRE: forward primer: tgtgccttggaatgctagt probe (FAM): tttggaatcacacgacct reverse primer...