Controlling for Off-target Effects with a New Genome-wide CRISPR Screen Design
Type
Blog Post
..., the toxicity of guides with off-target sites was profiled. Guides with exact or 1-bp mismatch off-targets...) or short (17-18 bp) guides. When comparing the toxicity of guides that have 1-bp mismatch off-target... in both screens, with the growth screen having a 1% false discovery rate and identifying >88% of previously...characteristics that influence gRNA off-target activity: 1) mismatches closer to the PAM are less tolerated than...Knockout Library are available from Addgene!
References
1. Morgens, David W., et al. "Genome-scale measurement...safe-targeting guides vs. non-targeting guides on screen analysis and hit calling. 5,644 non-targeting guides and...safe-targeting guides were included in the library. Safe-targeting guides were designed to target genomic...