...phone-sized, foldable microscopes that aren't very powerful (but are cheap and accessible) to massive transmission... are multiple objective lenses. Each objective performs essentially the same task but has different magnifying...scratches, and dust can easily impair microscope performance. Be sure to clean up your work area and microscope...

...transmembrane glycoprotein of the immunoglobulin superfamily, binds to the SARS-CoV-2 S protein and is involved...

...maize, Arabidopsis, and rice. Proof-of-concept performed in zebrafish. ZiFiT Targeter Originally developed...

...follow the kit's instructions. If you want to perform plasmid purification without using a kit, you can...ribonuclease that digests single-stranded RNA. (Optional) Perform phenol-chloroform extraction - see protocol below... DNA purified without a kit it is advisable to perform a phenol/chloroform extraction of the supernatant...

...Watch the protocol video below to learn how to perform Polymerase Chain Reaction (PCR). Basic PCR Program...pairs. The initial denaturation step is commonly performed at 94–98°C. Denature 30 seconds at 94°C: Continued...reagent by the number of individual PCRs you wish to perform and add 10% extra to account for pipetting error...

... of a stable cell culture after transduction. Performing antibiotic selection on transduced cells enables...5 dilutions plus one 'no virus' control well). Perform a "reverse transduction" by seeding 50,000 cells...untransduced well (0 µL lentivirus, above) are dying. Perform regular media changes and monitor the growth of...

...Models Induced pluripotent stem cells (iPSCs) are a powerful system for generating Rett syndrome-specific differentiated...

...Implementation Plasmids References The development of powerful molecular reagents for biology have propelled ...

... vivo testing of these viral preparations was performed by the Karpova lab. Results for our quality control...

...monoclonal cell lines. When this protocol was performed at Addgene on the A549 cells described in our ...expression and/or other phenotypes. For example, perform Western blotting to screen for lines with the highest...blasticidin selection. Anti-Cas9 Western blotting was performed on whole-cell extracts from the initial stable...

...scaling. Prokaryotes have long utilized CRISPR as a powerful defensive strategy against viral invaders, and...

...lentiviral CRISPR libraries (both human and mouse) for performing genome-wide knockout screens. The libraries are...

...contains five plasmids and one bacterial strain for performing a bacterial two-hybrid (B2H) screen of modularly...

...Mikko Taipale 12563 PCD FL0X Firefly Insertion of imperfect miRNA sequence in the 3' UTR Carl Novina 33154...

... Pooled Libraries Pooled libraries represent a powerful tool for forward genetic screening, or identifying...in the selection NEGATIVE screen: Apply library Perform Next Generation Sequencing (NGS) on a control sample...library Apply selection; most cells will survive Perform NGS on the surviving cells Compare NGS results ...

...Protocol, tips, and FAQ for how to perform a restriction digest of plasmid DNA...more enzyme is advisable. Reactions are often performed with 0.2-0.5 µL of enzyme because it is difficult...

...fragments. This reaction, called ligation, is performed by the T4 DNA ligase enzyme. The DNA ligase catalyzes...digested vector backbone with a phosphatase before performing the ligation reaction (phosphatase removes the...

... (from Chloromonas oogama ), and SdChR (from Scherffelia dubia ). Synthetic variants have been created...Chloromonas subdivisa CheRiff Channelrhodopsin from Scherffelia dubia Channelrhodopsins: cation channels- chimeras... opsin is reached. This approach is especially powerful when combined with site specific recombinase technology...

...Standard protocol for performing agarose gel electrophoresis, including tips to improve resolution and...Watch the protocol video below to learn how to perform gel electrophoresis. Equipment Casting tray Well...

...with purified enzymes, it takes advantage of the powerful recombination abilities of yeast. Similar to Gibson...than 10kb. Another advantage is the ability to perform oligonucleotide stitching, in which pieces of DNA...