...Reporter UnLOCKING (SHERLOCK and SHERLOCKv2) (Gootenberg et al., 2017 and 2018). While the Doudna lab ...not been approved for diagnostics yet, but is undergoing validation studies.     Jennifer Tsang contributed...updating this article. References Abudayyeh OO, Gootenberg JS, Konermann S, Joung J, Slaymaker IM, Cox DBT...439 . https://doi.org/10.1126/science.aar6245 Gootenberg JS, Abudayyeh OO, Lee JW, Essletzbichler P, Dy...442 . https://doi.org/10.1126/science.aam9321 Gootenberg JS, Abudayyeh OO, Kellner MJ, Joung J, Collins...10.1126/science.aaq0179 Myhrvold C, Freije CA, Gootenberg JS, Abudayyeh OO, Metsky HC, Durbin AF, Kellner...Shmakov S, Abudayyeh OO, Makarova KS, Wolf YI, Gootenberg JS, Semenova E, Minakhin L, Joung J, Konermann...

...other reagents in biology. Luk H. Vandenberghe, PhD Luk Vandenberghe’s first lab experience was in a virology... Khandan Baradaran, Frank Stegmeier, and Luk Vandenberghe. We’re excited to grow the team and bring on...worked on vaccines throughout that time. Now Vandenberghe is an Associate Professor at Mass Eye and Ear... to develop an AAV-based COVID-19 vaccine.  Vandenberghe’s work with AAVs led to many interactions with...ultimately make innovation and science slow,” Vandenberghe says. “The continuous creativity that Addgene...Karen Guerin for the journal Molecular Therapy. Vandenberge is also the Grousbeck Family Chair in Gene Therapy...

...Plasmids: History & Biology The Joshua Lederberg Papers Joshua Lederberg's Personal Perspective: Plasmid (1952...Pubmed PMID: 13003535. Lederberg, Joshua, Luigi L. Cavalli, and Esther M. Lederberg. “Sex Compatibility ...did plasmids get their name? In 1952, Joshua Lederberg set out to clarify the classification of these...the Fertility, or F-factor discovered by Ester Lederberg in 1952 was noted to integrate into the E. coli...PubMed Central PMCID: PMC3785787. References: Lederberg, Joshua. "Cell Genetics and Hereditary Symbiosis...

...Bioluminescence Resonance Energy Transfer CRET= Chemiluminescence Resonance Energy Transfer    Interesting...well-known FRET, aka Fluorescence (Förster) Resonance Energy Transfer, technique which allows the detection ..."a distance-dependent physical process by which energy is transferred non-radiatively from an excited ... may be higher than the signal generated by the energy transfer. On the contrary, if you choose a pair... development of new fluorescent pairs and the emergence of faster measurement systems, this technique .... Sekar RB, Periasamy A. Fluorescence resonance energy transfer (FRET) microscopy imaging of live cell...

...standard viral production , these viral vectors undergo quality control, including AAV titering by probe-based...

...summer internship information sessions to help undergraduate students with their applications. The society...society currently has 1,283 members, who comprise undergraduates, graduate students, postdocs, faculty members...The society encourages interactions between undergraduates and PhD students so that they can help support...society currently has 352 members, ranging from undergrad all the way up to professors. Oxford Biological...at Imperial College London for students in this emerging field. It has gained over 170 members in its first...or biological societies were you a part of in undergrad or grad school? How did these societies help (...

...blogger Iris Lindberg, Professor at the University of Maryland School of Medicine. In the Lindberg Lab we ...blogger Iris Lindberg, Professor at the University of Maryland School of Medicine. Iris Lindberg is a professor...PC12, Rin5F, Neuro2A and GH4C1, among others (see Lindberg papers in PubMed). While we found that nearly ...neural and endocrine cells. She can be emailed at ilindberg@som.umaryland.edu.   Additional Resources on the...

...Lab vectors - Tools for nematode transgenesis Rosenberg Lab Plasmids - Transgenic single-copy...

... biomedical research. Bockamp E, Maringer M, Spangenberg C, Fees S, Fraser S, Eshkind L, Oesch F, Zabel...

... any bacteriologist’s skill set. So when Dave Westenberg taught this concept in his microbiology lab course...wear a mask. (@GermJuggler) February 13, 2021 Westenberg, a Professor of Biological Sciences at Missouri...by using a thicker bacterial suspension,” says Westenberg. In addition to streaking for single colonies...them into different strains of E. coli,” says Westenberg. During the transformation experiment, he plans...efficiency.  Aside from the Streak Plate Challenge, Westenberg’s course includes Winogradsky columns and characterizing...

...information, see the Addgene blog post from the Chan Zuckerberg Biohub . gRNA Design Deep learning models to ...

...TCATGGTGCAGCCGTTACtggacagccgcgttccCTACGGACGCCTCCAGCA Purinergic receptor Y2 TAL3348 & TAL3349 TTCAACGCATCAGTGAATgttcaaaaccacagttCTGCACACAGCTGCAGCA...

...fluorescent microscopy. Collimated (non-converging or diverging) excitation light exits the microscope ...its unrivaled contrast, fluorescence imaging has emerged as the dominant light microscopy contrast technique...GFP. Because red light has approximately half the energy of blue light, two photons of red light are needed...Although the excitation light is completely reflected, energy is propagated into the sample via an evanescent...the fluorophore’s interaction with an evanescent energy field. Lightsheet microscopes that project ultra-thin...

...after undergrad] was to learn and speak the language of science. I didn’t come out of [undergrad] feeling...solidified some concepts that are heard often during undergrad. Lauren Flynn is the Addgenie behind our...doctor. After taking a year break in between undergraduate school and studying for the MCAT, she decided...description.” Our resident bagel provider, source of energy, and conversation master knows that although her...

...science.   Acknowledgements Chan Zuckerberg Initiative Chan Zuckerberg Biohub Manuel Leonetti Ryan Leenay...Greg Dingle, a software engineer with the Chan Zuckerberg Initiative. We hereby announce the general availability... us your questions or comments to gdingle@chanzuckerberg.com. We're eager to hear your thoughts. Many...Greg Dingle is a software engineer with the Chan Zuckerberg Initiative, working on assignment at the Biohub...

...Cressey is a PhD Student in the labs of Dr. Elke Mühlberger and Dr. Rachel Fearns at Boston University School...Medicine, Department of Microbiology, National Emerging Infectious Disease Laboratories.  She is interested...The power of reverse genetics. Springer Berlin Heidelberg, 2004. 1-41. PubMed PMID:15298166. 3. Hoenen,...21699921. PubMed Central PMCID: PMC3586226. 4. Mühlberger, Elke, et al. "Comparison of the transcription... 9971816. PubMed Central PMCID: PMC104478. 5. Mühlberger, Elke. "Filovirus replication and transcription...

...1147. (Link opens in a new window) PMID: 17289941 Hagberg et al. 1983. A progressive syndrome of autism, ...

...standard viral production , these viral vectors undergo quality control, including AAV titering by SYBR...

...skills, but I’ve learned a lot since my days as an undergrad working with cancer cell lines. As I started planning...useful tasks. The quick accomplishments will likely energize you. For example, let's say you’re doing a multi-day...that should help keep you content and give you the energy to finish any additional work you might need to... all if I’d planned appropriately. Back in my undergrad days, I had general goals for my experiments, ...to it each day. Once you get over the activation energy barrier that comes with making ANY progress, the...

...intracellular domain of an endogenous GPCR, such as adrenergic receptors. When exposed to light, there is a ...Figure 3: Chimeric OptoXR incorporating rhodopsin undergoes a conformational change when exposed to blue-light.../978-1-0716-0755-8_2 Boyden, E. S., Zhang, F., Bamberg, E., Nagel, G., & Deisseroth, K. (2005). Millisecond-timescale..., N., Berthold, P., Ollig, D., Hegemann, P., & Bamberg, E. (2003). Channelrhodopsin-2, a directly light-gated...Dieter, A., Pieper, F., Sims, R., Tenedini, F., Bergs, A. C. F., Bendifallah, I., Zhou, F., Zeitzschel...