... was designed in response to requirements that emerged from intensive engagement with a wide range of ...research computing. The two guiding principles emerging from the engagement with the Wisconsin research...fundamental driver of these principles and has emerged as a byproduct of connectivity. Between 2012 and...

...approach, Zhang et al. turned to the CRISPR/Cas9 Synergistic Activation Mediators (SAM) system available from...Young WB, Khalili K, Hu W. CRISPR/gRNA-directed synergistic activation mediator (SAM) induces specific, persistent...Wang Z, Pan Q, Gendron P, Zhu W, Guo F, Cen S, Wainberg MA, Liang C. CRISPR/Cas9-Derived Mutations Both...

...experiments (Rolland et al., 2016; Su et al., 2004; Taschenberger et al., 2017).     Figure 3: Comparison...C. M., Cheng, F., Qiu, J., Carette, J. E., & Vandenberghe, L. H. (2018). An Alternate Route for Adeno-.... https://doi.org/10.1007/s11064-004-6881-1 Taschenberger, G., Tereshchenko, J., & Kügler, S. (2017). ...

...there is a lack of expert mentorship during undergraduate and graduate studies. Students also need more... a rapidly changing, globalized environment re-emerging from international financial struggle, our new...mentorship in the field of Biomedicine to 78 undergraduate/graduate students from 5 independent universities...

...delivery methods, but since TEs insert mostly in intergenic regions, gene disruption is less of a concern...to a single location in bacterial genomes, the Sternberg lab developed the INTEGRATE (Insertion of Transposable...transposons-the-jumping-genes-518/   Sandoval-Villegas N, Nurieva W, Amberger M, Ivics Z (2021) Contemporary Transposon Tools...

...has been demonstrated that certain serotypes can undergo axoplasmic transport. These AAVs may move toward...Examining serotypes 1, 8 and 9, not only do they undergo retrograde and anterograde transport, but they ...avoid this rate limiting step and can immediately undergo replication and transcription. However, with scAAV...

...both methods did a more comprehensive gene list emerge that accurately reflected the scope of genes known... straightforward positive selection of plants undergoing RNAi, and LIIbeta F 1-2 RNAi, which allows assembly...microscopic plants must be visually identified as undergoing RNAi through the fluorescent reporter and manually...

...substrate, luciferin, which releases energy as a photon. The photon energy is transferred to the acceptor fluorophore...pcDNA3.1+-pHlash, that use Bioluminescence Resonance Energy Transfer (BRET) were developed by the Johnson Lab...fusion protein (referred to as dCas9-VPR) had a synergistic effect on target gene activation. The novel dCas9...rely on the principle of bioluminescence resonance energy transfer (BRET), whereby photons emitted by a Renilla...

...permeabilization with detergents such as triton-x, saponin, or tween. Take care when using strong detergents like triton-x...resources References: Bonner WA, Hulett HR, Sweet RG, Herzenberg LA (1972) Fluorescence Activated Cell Sorting...

.... Two low-energy photons (typically from the same laser) cooperate to cause a higher-energy electronic...photoconvertible Keade proteins (protein that undergoes light-induced irreversible photoconversion from...

...sgRNA-scaffold 47912 Worm AflII none S. pyogenes Sternberg pJW1311 61254 Worm none, fusion PCR none S. pyogenes...

...aware of our own consumption. Quick ways to reduce energy consumption in the lab Here are a few easy and ...autoclaved together. The autoclave uses a lot of energy, so even one less run a week makes a big difference...

...accounts for many, many lattes and espressos. The energy boost is well needed to keep up with all the awesome...great opportunity – so if you’re running out of energy, then it’s time for a coffee run! ...

...Preparing electrocompetent cells (protocol)  Untergasser Lab Preparation of chemically competent cells... (protocol)  Electroporation (protocol)  Untergasser Lab Transformation of chemically competent cells...

...against parasites and is responsible for driving allergic reactions such as anaphylactic shock Monomer with...

...inactivation details, tissue preservation method, and detergents that they used in their experiments. Researchers..., method, tissue preservation, target tissue, detergent, antigen retrieval conditions, and more. Users...

...effort was led by two amazing postdocs, Kristin Krukenberg and Jessica Polka from Harvard Medical School...Bourne charged the audience to use their useful energy and to choose institutions with allies engaged ...

...spondylarthritis, using Bioluminescence Resonance Energy Transfer (BRET) technique, and plasmids to study...and worked around the world, carrying out her undergraduate work at the University of London and the University...

...being opened in the pathogen’s cell membrane (Dunkelberger & Song, 2010). As cells cannot survive when ...):443-6. doi: 10.1038/74704. PMID: 10742152. Dunkelberger, J., Song, WC. Complement and its role in innate...

...understand about Dopamine? DA is produced by specific dopaminergic neurons in a brain region called the substantia... Report endogenous DA release from a single dopaminergic fiber in mouse brain slices Demonstrate DA detection...