... maintain this plasmid collection page, which highlights plasmids available that may be useful for COVID...called SARS-CoV-2. Scientists have been working at lightning speed to elucidate as much as they can about this... Ginkgo Bioworks Blog post: Synthesizing DNA to fight coronavirus (Link opens in a new window) . The collection... develop and optimize the method termed Specific High Sensitivity Enzymatic Reporter UnLOCKING (SHERLOCK... now available from Xiaokun Shu's lab. A rapid, highly sensitive and open-access SARS-CoV-2 detection ...Transcriptase plasmids from Andrew Ellington's lab. High-surety isothermal amplification and detection of...

...mApple-VE-Cadherin-N-10 Tight Junctions VE-Cadherin mApple Michael Davidson 55165 mCherry-ZO1-C-14 Tight Junctions ...vesicles Clathrin, light polypeptide mRFP Ari Helenius 70217 pEGFP-GFP11-Clathrin light chain Clathrin-coated...cell junctions: adherens junctions, gap junctions, tight junctions, and desmosomes. Browse or search the ...Beta-Catenin mCherry Michael Davidson 30313 pEGFP ZO1 Tight Junctions Zonula Occludens-1 GFP Alan Fanning *Fusions...-coated vesicles Clathrin light chain GFP Bo Huang 54040 mEmerald-Clathrin-15 Clathrin-coated vesicles...Clathrin-LCa-EYFP Clathrin-coated vesicles Clathrin, light polypeptide EYFP Xiaowei Zhuang 14437 mRFP-Rab5 ...

...We provide high quality AAV preps from select plasmids in the repository. Browse the University of Florida...and T492V. AAV44.9 The AAV44.9 serotype displays highly efficient transduction and lateral spread in the...Arun Srivastava and cite Petrs-Silva, et al. 2009. High-efficiency transduction of the mouse retina by tyrosine-mutant...a mouse model of complete congenital stationary night blindness. Hum Mol Genet . Nov 1;24(21):6229-39....acknowledge Arun Srivastava and cite Ling, et al. 2016. High-Efficiency Transduction of Primary Human Hematopoietic...27759036 Other citations include: Ling, et al. 2016. High-Efficiency Transduction of Primary Human Hematopoietic...

... display less off-target activity. High fidelity Cas9s: Three high fidelity Cas9 variants , SpCas9-HF,...95% of archaeal and 48% of bacterial genomes, are highly diverse, with varying PAM sequences and number/...) Type V (Cpf1) Type IV (Csf1) Type VI (Cas13) Fighting Back: Anti-CRISPR Genes in Phage The CRISPR adaptive...creating a double-strand break. This method displays higher efficiency than HDR-mediated editing. The first... Genome engineers continue to work to develop a highly specific, programmable platform well-suited for...MS, Tsai SQ, Nguyen NT, Zheng Z, Joung JK. 2016. High-fidelity CRISPR-Cas9 nucleases with no detectable...

...that it is responsive to specific stimuli, such as light. On a broader scale, the expanded genetic code can...included. Browse Synthetase Plasmids The table below highlights plasmids that contain aminoacyl tRNA synthetase... David Tirrell Browse Strains The table below highlights bacteria strains that have been modified to enhance...Ryan Mehl Browse Target Plasmids The table below highlights plasmids that contain genes with modified codons...

...deletion-mutant rabies virus AAV1 Wickersham 100799 pAAV-TREtight-mTagBFP2-B19G AAV1 Wickersham Introduction to ...tracing virus that lacks the ability to infect neighboring cells. For example, G-deleted rabies lacks the...starter" cell, but new virus cannot spread to any neighboring cells. To enable monosynaptic spread of this ...newly formed viruses are unable to infect any neighboring cells. Thus, through complementation, G-deleted...released from the starter cell and free to infect neighboring cells via G-protein receptors, which are naturally...

...cancer cells. Adenoviruses have high transduction efficiency and direct high but transient transgene expression...clinical trials. These viruses have a relatively high transduction efficiency, though this infectivity... can be delivered to a host and generate virus (right), similar to how natural viruses can be delivered...stranded linear DNA >8 Kb 105 Dividing/Non-dividing No High γ-Retrovirus Stable RNA <8 Kb 80-130 Dividing Yes...

...for tetracycline transregulation. Dox binds with high affinity to tTA and rtTA; thus, dox can be used ...plasmids third-generation rtTA On Elledge 19407 pTREtight2 Promoter contains a modified TRE that is silent...silent in absence of binding tTA or rTA; has high copy E. coli origin None Either Ralser 64238 pTet-IRES-EGFP...Expresses firefly luciferase hairpin and GFP under pTREtight promoter None Either Elledge 35625 pAAV-Ptet-RFP-shR-rtTA...promoter and enhancer options tTA Off Uney References Tight control of gene expression in mammalian cells by...

...Kita Kit a 58949 pVk7-33G1-BioLHis IGHC1 58950 pVk7-33G1-Flag IGHC1...Monoclonal Antibodies You may also like... Gavin Wright Lab plasmids This is a collection of plasmids that...secreted proteins. The plasmids encode both the light and heavy chains of mouse monoclonal antibodies ...

...types and/or developmental timeframes. Here we highlight some of the plasmids and tools available at Addgene...Plasmid Tools for Use in Worms The table below highlights plasmids that contain tools for use in worm research...

...optimized for each specific virus in order to generate high quality preparations. After production, all virus... that titers obtained using ddPCR are generally higher than those achieved using the standard qPCR method...method. Quality Control Addgene ensures high quality viral vectors by optimizing and standardizing production...staining or SYPRO Red staining and the molecular weight and relative intensity of the viral capsid proteins...

...joining fragment comprising a κ light chain constant domain (C L ) and the κ light chain polyA tail sequences... for light chain expression, and an ER signal/leader sequence (L) for translocation of the light chain...in a new window) Durocher Y, et al. 2002. High-level and high-throughput recombinant protein production... R-mAbs are secreted into the culture media. For high level expression we recommend using the HEK-2936E...Link opens in a new window) . The schematic to the right shows the separate elements of the R-mAb expression...expression plasmid involved in coexpression of light (green) and heavy (blue) chains as driven by two CMV promoters...

...mutations are known to cause Rett syndrome, however, eight recurrent missense and nonsense mutations (R106W... labs Plasmids MECP2 Plasmids The table below highlights plasmids that express or target the MECP2 gene... 55–67. PMID: 30536762 Shahbazian et al. 2002. Insight into Rett syndrome: MeCP2 levels display tissue...

...that wildtype Cas9 with optimized chimeric gRNA has high efficiency but has been shown to have off-target...prefers a 'G' at the transcription start site to have high expression, so adding this G could help with expression... culture unless the targeting efficiency is very high. How should I design experiments for multiplexed...but the efficiency will be lower, although this might still be okay if you are introducing a selection...

... Zebrafish Receptors You may also like... Gavin Wright Lab plasmids This is a collection of plasmids that...neural leucine-rich repeat receptors. Sollnet C & Wright GJ. Genome Biol 2009;10(9):R99 PubMed 19765300 ...essential for vertebrate myocyte fusion. Powell GT & Wright GJ., PLoS Biol. 2011 Dec;9(12):e10012165. PubMed...

...Service: AAV Addgene's viral service provides high-quality and high-titer viral preparations. Addgene's AAV ...are pleased to announce a partnership to provide high-quality AAV vectors to the academic research community...

... for each specific antibody in order to generate high quality preparations. Following production, all ...transfected with the plasmid(s) encoding the heavy and light chains. Antibodies are expressed for 7 days and ...amplified with primers targeting the heavy chain and/or light chain variable regions. The PCR product is purified...

...double nicking, however, increases indel formation slightly. Designing the sgRNA with a spacer that only binds... gRNA vectors suitable for prime editing are highlighted in the table below. Use the search bar to find...

...Vector Core) and Addgene have partnered to provide high-quality AAV vectors to the academic research community...Deisseroth AV-1-36857M 100038-AAV1 pENN.AAV.hSynap.Flex.ArcLightDco.WPRE.SV40 Vincent Pieribone AV-1-36857P...36857P 100037-AAV1 pAAV.hSynap.ArcLightD.WPRE.SV40 Vincent Pieribone AV-1-51473 51473-AAV1 pAAV-hSyn1-mRuby2...James M. Wilson AV-1-PV2699 100030-AAV1 pAAV.CBA.loxP.ArcLightD.2A.nlsmCherry.loxP.WPRE.SV40 Lawrence Cohen...

... are able to recognize their targets with fairly high specificity. Fifteen years of research has shown...TALe research has been swift. The incentives are high. Since ZF targets are confined to sequences composed...on specificity is on par with ZF arrays, if not slightly lower. Figure 2: Simplified representation of ...