...Human Platelet Receptors You may also like... Gavin Wright Lab plasmids This is a collection plasmids for ...Platelet Receptor Protein Microarray Identifies the High Affinity Immunoglobulin E Receptor Subunit α (FcεR1α...

...and C terminus and induces mutation at rates much higher than the parental vectors. pC-GoldyTALEN directs...obligate heterodimers that require cloning of left and right TALEN monomer proteins into opposite vectors. 37276...

...both P-elements and attB site for high expression of UAS-driven transgenes Worm unc-54,...Gateway cloning vectors Adenoviral High transduction efficiency, but do NOT integrate into...

...engineering using the CRISPR-Cas9 system. Ran FA, Hsu PD, Wright J, Agarwala V, Scott DA, Zhang F. Nat Protoc . ... engineered CRISPR-Cas9 complex. Konermann S*, Brigham MD*, Trevino AE, Joung J, Abudayyeh OO, Barcena...

...Parkinson's Richard Youle 23966 pTreTight-Htt94Q-CFP HTT CFP TREtight Huntington's Nico Dantuma 23971 ...Alzheimer's Gavin Wright 51643 APP-bio-His APP His, CD4 CMV Alzheimer's Gavin Wright 51811 PRNP-bio-His...PRNP-bio-His PRNP His, CD4 CMV Dementia Gavin Wright 53363 pCB268 APP Flag, HA, Myc CMV Alzheimer's Alexander Varshavsky...132389 AAVS1 CAG rtTA3 TauWT 2N4R-EGFP MAPT GFP TREtight Parkinson's, FTD Gerold Schmitt-Ulms 132393 AAVS1...AAVS1 CAG rtTA3 TauP301L 2N4R-EGFP MAPT GFP TREtight Parkinson's, FTD Gerold Schmitt-Ulms 133320 pHBS834...

...Protein Activation Library 113345 Activation Human Wright 3rd 7-14 58,570 Human miRNA CRISPR Knockout Library...

...based on using a thin plane (sheet) of light generated by sunlight to observe single gold particles with...V= ventral Developments in light sheet fluorescence microscopy Modern light sheet fluorescence microscopy...out-of-focus reflected light by using a pinhole.  However, the excitation light excites all of the fluorophores..., there is a drop in image brightness with increasing depth caused by light scattering and absorption....of this century has seen some major advances in light microscopy, particularly related to the neurosciences....  One of these advances in microscopy has been light sheet fluorescence microscopy (LSFM). The underlying...a fluorescent sample and captured the reflected light using a different objective lens oriented perpendicular...

...antibodies have higher affinity and specificity than mouse monoclonal antibodies. Antibodies with higher affinities...monoclonal antibodies contain a single clone they have higher lot to lot specificity and tend towards more reproducible...When using a monoclonal antibody be sure to use a highly specific secondary antibody, rather than a broadly...at very low levels or your application requires a high degree of affinity, then species choice could very...may be the better choice for assays that require high affinity binding, such as immunoprecipitations. ...stain a target protein in mouse tissues, causes a high degree of background staining. This occurs because...antibody isotypes matter and helps you choose the right antibody for your application!  References and ...

...experiments require higher gene expression and therefore higher titer than others. High titers are often ...vector to such a high titer when looking for effective gene expression. How do you achieve high titers in the...good ones into a cell to produce expression. The higher the titer, the better. AAV transport and transgene...transgene expression It’s clear that we need high titers, as contact between the cell and any individual AAV...the cell may not always be enough and therefore higher titer will be required. For example, retrograde...over an even larger area, thus necessitating even higher retrograde AAV titers for successful transduction...That means that once you drop below a certain (very high) threshold of viral particles per cell, you may ...

...data on the assay methods. You might find that while the website highlights a variation of your application...protein or protein region highly conserved across species? If the proteins are highly homologous between your...

...the pressure to publish in high-impact journals, and the requirement for a high grant success rate. At this... this point, candidates are highly experienced but often have to start pursuing other options. While some...technical support specialist for 4 years. Read on for insights from my time in technical support including tips..., and resolve complaints about products. As you might expect, different companies produce different products... dialogue. This will give you a feeling of what might be involved in such a role. In addition, it can ...individual stories and get (sometimes refreshing) insights into how other scientists think and work. Most...to enhance the OD signals and then complain about high background. In many instances, variations of the...

...fluorescence when exposed to 500 nm light.  Under 400 nm cyan light, Dronpa dimers dissociate, Dronpa goes...minute pulse of 500 nm light (turn on psRaf1) followed by 3 seconds of 400 nm light (turn off psRaf1). After...and use MEK1 as a model kinase. In this system, light is used to turn “on” and “off” MEK1 activity by ...is called psMEK1. When exposed to 500 nm violet light, the Dronpa dimers interact and lock MEK1 in an ... an inhibitor of choice, exposed to 500 nm cyan light to activate psMEK1, and the localization of ERK ...these same cells by 24-48 hours exposure to blue light also induced tail swelling in ~75% of worms (see...expressed in a tissue or organism that blue and violet light can penetrate. How can I try using photoswitchable...

...for many years (at a very low salary I might add). You have a right and responsibility to choose a lab where...Why is choosing the right lab such a big deal? It’s actually something you CAN choose and it will make...difference for your future career and life. You might see a lab head as choosing you, but in reality, ...communication before making this important decision. I’ll highlight some of these questions in the sections below....you are good at asking for and getting help, size might not be a big factor for you. Know who's boss You...considering a career at a small, teaching college you might want to consider less expensive, portable areas ...ownership" and author order PI lets lab members fight it out PI intervenes if there are interpersonal...

...excitation light less efficiently. Similarly, decreasing the power of the excitation light reduces the...series described a practical approach to selecting a bright fluorescent protein. In the second post of this...destruction of a fluorophore under the influence of light. Any fluorescent molecule will photobleach at some...fluorescent proteins are excited with very intense light (for a brief time), whereas in widefield imaging...imaging fluorophores are excited with relatively low light levels (for a prolonged time). Other illumination...that, in experiments that measure photostability, high excitation powers are often used to reduce the length...linearly related to power, the conclusions reached at high power may not translate to real applications in ...

... short generation times and high fecundity, make zebrafish ideal for high throughput studies of optogenetics...Optogenetics, the use of light sensitive proteins (opsins) to manipulate cell activity, enables researchers... studies because it is non-invasive and offers a high degree of control over time and space. Zebrafish...imaging with fluorescent tags and activation of light sensing proteins. Furthermore, during the first ...Optogenetics functions through opsins which are light sensitive proteins. Opsins used in optogenetic studies...particles to cross the cell membrane when exposed to light. Neuronal firing depends on these charged particles...of the differing peak absorption wavelengths and light intensity requirements of different opsins, studies...

...solution for discriminating between neurons for light microscopy-based neuronal reconstruction. To achieve...et al., 2007; Cai et al., 2013). However, the brightness of Brainbow was inherently low. This is because...at ~2 copies per cell per color to achieve the highest color variations. Adjust the plasmid concentrations... to express a minimal amount of tTA to achieve highest expression levels. Clearing large tissues - Fluorescent...anatomical aspects of sensory circuit development using light microscopy. Lab website: https://dn-en.med.kyushu-u.ac.jp...clearing and index matching of tissue samples for High-resolution fluorescence imaging using SeeDB2." Bio-Protocol...Sakaguchi, Richi, Marcus N. Leiwe, and Takeshi Imai. "Bright multicolor labeling of neuronal circuits with fluorescent...

...regulatory elements, pre-assembled genetic circuits, and higher level gene networks. Examples Include: promoters...

...Basic AVEXIS Plasmids You may also like... Gavin Wright Lab plasmids AVEXIS is an avidity-based extracellular...

...ever used Golden Gate Assembly for cloning, you might be familiar with the rules of thumb for designing...way to design GGA overhang sets, particularly for high-complexity reactions? The researchers over at New...otherwise! They suspected a data-driven approach might allow for an increase in the complexity. In 2018...need to be strictly followed in order to generate high-fidelity reactions. (Need a refresher? The five ...100% GC.) In fact, the NEB® team’s data showed that high-fidelity reactions could be done using overhang ...sets that broke rules 3—5. Fidelity, it turns out, might depend more on the individual sequence(s) than on...build out three tools to help researchers design high-fidelity Golden Gate Assemblies and overhang sets...

...Investigators, an open-access journal that enables high school students to publish peer-reviewed scientific...behind the journal and hear from a fantastic team of high school students who recently submitted to the journal...Journal of Emerging Investigators. Chapter 2 - Getting high school students and teachers interested in JEI (...mentorship programs. Chapter 6 and Outro - Interview with high school student researchers Arooba Ahmed, Jiachen...