...2 Here! We'd like to thank Niroshi and Ben for taking the time to sit down and talk to us and wish them...

...group in industry? The collaborative nature of working in industry and how delegating can empower people...she sets metrics for success. The importance of thinking about the big picture and not just the next experiment...Chapter 6: Advice to scientists who are planning on working in industry. The importance of recognizing and ...

...genome randomly, while others use attP sequence docking sites so that you can control exactly in which ...at different loci. Fortunately, there are many docking sites available for integration. 2. How soon would...would know beforehand on which chromosome your docking site is located). This amounts to a small difference...important to note so that you know what to look for. Making your transgenic fly and downstream experiments ...experimentation. Drosophila are amenable to many different kinds of experimental assays depending on your interests...University. He has had nearly a decade of experience working with Drosophila as an experimental system. Jon ...Central PMCID: PMC1526508. 2. H. Jiang,  et al. Cytokine/Jak/Stat signaling mediates regeneration and homeostasis...

...importantly, this knowledge will stop you from embarking on a fruitless journey of trying to get males ...that. Now you have the basic knowledge to start working with flies. Like Addgene, the Drosophila community...chromosomes to use. If you’re not ready to start working with live animals yet, Harvard’s DRSC/TRiP- Functional...University. He has had nearly a decade of experience working with Drosophila as an experimental system. Jon ...

...need to be cost and time efficient. We do this by making our own competent cells and using a little-known...efficiency of transformation (for example, if you are working with a library or an especially low concentration...will work just fine.  A number of protocols for making competent cells are available online. A few examples...

...here and you can find more tips and protocols for working with lentivirus on our blog and protocol pages.... love to hear from you if you have used or are thinking about using our viral services. Please send any...

...mouse and human kinomes. While any subset of the genome is limited by definition, the kinome is a well-annotated...genetic screening project can be a tremendous undertaking, producing a wall of results that can only be... About CRISPR Pooled Library Screening Why the kinome? While properly designed perturbations – in the...incremental approach is prudent. What's in the CRISPR kinome library? Thus, we are glad to provide CRISPR libraries...pools provided in two sets, each with 4 gRNAs per kinase, so screens can be performed with either 4 or 8...individual lentiGuide gRNA plasmids targeting the human kinome. These plasmids contain the same 3-4 gRNAs found... Additionally, this set can be used for cherry-picking individual gRNAs that score as hits in pooled screens...

...has been supported by a Steering Committee and working groups with representatives from all areas of the...

... I found Vectr to be fairly intuitive and I was making figures quickly. Inkscape Inkscape is the most...images. For example, by selecting “Tools” and then clicking the “Usage rights” dropdown menu, you can search...

...fluorescent proteins to oligomerize is known as “stickiness.” Since the fluorescent tag should operate as... per unit.  There are several ways to measure stickiness. In vitro, ultracentrifugation or gel filtration...oligomerization in cells. In addition to measuring stickiness, several researchers have also collected data...characteristics are important, but have less priority than stickiness. In my experience, fluorescent proteins that ...independent of GDI-mediated extraction and actin-based trafficking." PLoS biology 13.4 (2015): e1002097. PubMed ... measurement of Gαi1, Gαi2 and Gαi3 activation kinetics in single cells." PloS one 11.1 (2016): e0146789...

...really fit in anywhere and everyone who was doing the kind of work my lab wanted to do already seemed to know...graciously took me for coffee and spent an hour with me talking about the tools I could use, and some experimental...This was invaluable to me as a bench scientist working at an institution where training opportunities ...network at the meeting. We found our mentors by asking for help with the program on social media. Many...between mentors and Singletons can accelerate the networking timetable for the Singletons in their bins.  ...organizers in their town hall.  Iterative improvement - Making Binning Singletons better Our experience running...leaps. Binning Singletons is its own organization, working to make scientific meetings more productive for...

...differently if I would be speaking to a colleague in the office next door. So be kind, helpful, and thorough...

...University. In this second post in our quick guide to working with Drosophila, you’ll learn how to maniupate ... YFG. You could compare this mutant fly to one lacking the mutation in the same genetic background. In...express YFG in a new tissue type (more details on making a fly line in the next blog post). For example,...get the right cell type expression as opposed to making a new fly with hedgehog under a new promoter each...University. He has had nearly a decade of experience working with Drosophila as an experimental system. Jon ...

...where I spent a couple of days talking to researchers about their work, picking up plasmid deposits, and trying...spend a large amount of my time meeting with and talking to graduate students, post-docs, and PIs. I won...enterprise work better and because I really like talking to people about and communicating science. The ...research at BU, I realized that I’m energized by talking to people about science and that I love explaining...running away screaming, but instead can involve walking alongside it and giving it a high five. While screaming...technical writing, teaching, presenting, analytical thinking, time management, information distillation, etc...transferable to many different fields. If you start thinking about preparing yourself for a career while still...

...LRRK2 His, Myc CMV Parkinson's Ted Dawson 17612 pRK5-Myc-Parkin PRKN Myc CMV Parkinson's Ted Dawson 17613...William Hahn 23955 YFP-Parkin PRKN YFP CMV Parkinson's Richard Youle 23956 mCherry-Parkin PRKN mCherry CMV ...2XMyc-LRRK2-Kinase LRRK2 Myc CMV Parkinson's Mark Cookson 25072 2XMyc-LRRK2-LRRs LRRK2 Myc CMV Parkinson's Mark...His polH Parkinson's Michael J Fox Foundation MJFF 40385 pBACgus4x/Parkin PRKN polH Parkinson's Michael...PINK1 Myc CMV Parkinson's Mark Cookson 13314 pCMVTNT PINK1 C-myc PINK1 Myc CMV Parkinson's Mark Cookson...PINK1 GFP CMV Parkinson's Mark Cookson 13316 pcDNA-DEST47 PINK1 C-GFP PINK1 GFP CMV Parkinson's Mark Cookson...PINK1 V5 CMV Parkinson's Mark Cookson 13319 pLenti6-DEST PINK1-V5 KD PINK1 V5 CMV Parkinson's Mark Cookson...

...repeat cycles of expansion. Chemokines Chemokines, or chemotactic cytokines, are small proteins that can...other cytokines, enable communication between cells of the immune system. Interleukins Interleukins are ...factor FLJ40431 Return to top Chemokines Chemokines, or chemotactic cytokines, are small proteins that can...CCL14-CCL15 chemokine ligand 14, chemokine ligand 15 transcription unit - CCL15 chemokine (C-C motif) ...CCL27 chemokine (C-C motif) ligand 27 ALP, CTACK, CTAK, ESKINE, ILC, PESKY, SCYA27 CCL28 chemokine (C-C...CX3CL1 chemokine (C-X3-C motif) ligand 1 ABCD-3, C3Xkine, CXC3, CXC3C, NTN, NTT, SCYD1, fractalkine, neurotactin...SCYB14, bolekine CXCL16 chemokine (C-X-C motif) ligand 16 CXCLG16, SR-PSOX, SRPSOX CXCL17 chemokine (C-X-...

...Mitogen-activated protein kinase kinase MET MET proto-oncogene, receptor tyrosine kinase MLST8 MTOR associated...STK4 Serine/threonine kinase STK11 Serine/threonine kinase 11 TBK1 TANK binding kinase 1 TFDP TFDP1 TFDP2... CDK6 Cyclin-dependent kinase CNKSR CNKSR1 CNKSR2 Connector enhancer of kinase suppressor of Ras CYTH2...receptor tyrosine kinase 2 ERK MAPK1 MAPK3 Also known as MAPK; Mitogen-activated protein kinase ETS ETS1 ETS2...coiled-coil containing protein kinase ROS1 ROS proto-oncogene 1, receptor tyrosine kinase RPS6KA RPS6KA1 RPS6KA2... Ribosomal protein S6 kinase RPS6KB RPS6KB1 RPS6KB2 Ribosomal protein S6 kinase B1 RPTOR Regulatory associated...homolog 3 ALK Anaplastic lymphoma receptor tyrosine kinase ARHGAP35 Rho GTPase activating protein 35 ARHGEF2...

...nerve cell function within the cell. Parkinson's Disease Parkinson’s disease (PD) is a chronic and progressive...neurodegenerative diseases include Alzheimer’s, Parkinson’s, ALS, and Huntington’s Disease. These diseases...motor neurons leads to limb weakness, difficulty speaking, swallowing and breathing, and often results in...movements, memory deficits, as well as difficulty speaking and swallowing. HD is caused by abnormal expansion...Symptoms include memory loss, deficits in decision-making and language abilities. The disease can be classified...mammalian axons using lentiviral vectors expressing kinesin heavy chain 5 . Zamponi et al. Dev Cell. 2022 Jun...GRABDA or the calcium indicator GCaMP6s allows tracking of dopaminergic signaling and neuronal activity...

...growth and proliferation. mTOR, a serine/threonine kinase, is found in two complexes, mTORC1 and mTORC2...homolog LKB1 Also known as STK11; serine/threonine kinase 11 mTOR Mechanistic target of rapamycin NF1 Neurofibromin...S6K Also known as RPS6KB1; Ribosomal protein S6 kinase B1 TSC1/2 TSC1 TSC2 Tuberous sclerosis VHL Von ...LST8 homolog GSK3 GSK3A GSK3B Glycogen synthase kinase 3 MDM2 MDM2 proto-oncogene mSin1 Also known as ...as MAPKAP1; Mitogen-activated protein kinase associated protein 1 mTOR Mechanistic target of rapamycin ...; tumor protein p53 PDK1 Pyruvate dehydrogenase kinase, isozyme 1 PI3K Catalytic Subunits PIK3CA PIK3CB... PIK3R6 Phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic and regulatory subunits - Class I PKC...

...pegRNA + nicking sgRNA BbsI No Vermilion Norbert Perrimon pHSG1C3 Mammalian U6 pegRNA + nicking sgRNA BbsI...heteroduplex resolution. The process of double nicking, however, increases indel formation slightly. Designing...the edited strand, as in the PE3b system, guides nicking of the unedited strand only after the edit has ...promoter, the type of gRNA (e.g., pegRNA, epegRNA, nicking sgRNA), cloning enzyme, selectable marker, and ... No mRFP1 David Liu QPM-sgR (pTaU3) Plant TaU3 nicking sgRNA Eps3I + NcoI No Caixia Gao pYPQ141D-peg Plant...Tilmann Weber pnsgRNA Bacteria J23119 (BBa_J23119) nicking sgRNA No Tilmann Weber pPBT-peRNA_GG-Puro Mammalian...sgRNA-mCherry Mammalian, AAV hU6 + H1 pegRNA + nicking sgRNA No mCherry Hyongbum Kim pDAS12069_U6-pegRNA-mCherry...