...product: Run PCR to amplify your insert DNA. It is important to use a high fidelity taq polymerase to minimize.... The fidelity of the polymerase becomes more important the longer the expected PCR product is. You should...some DNA during the gel purification step, it is important to digest plenty of starting material. We recommend...be cut with both enzymes, and therefore it is important that the digest goes at least 4 hours and as long...running a gel for purification purposes it is important to have nice crisp bands and to have space to ...expected. When cloning by PCR, it is especially important to run the product on a gel. This allows you to...insert and vector bands away from the gel, it is important to determine the concentration of recovered DNA...