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Showing: 21 - 40 of 96 results

  1. Cre-lox system

    Type
    Collection
    ...Youle 59720 Nanog-CreER targeting construct Cre-ERT2; Targeting vector for Nanog locus mNanog Mammalian ...

  2. Five Popular Model Organisms

    Type
    Blog Post
    ...fly (Drosophila melanogaster) Another popular model organism is Drosophila melanogaster, or as it is more...Nichols. "Human disease models in Drosophila melanogaster and the role of the fly in therapeutic drug ...

  3. Six Spooky Science Stories and Halloween at Addgene

    Type
    Blog Post
    ...cytochrome P450 genes discovered in Drosophila melanogaster (Gilbert, 2004). Mutations in these genes are...mediate steroid hormone biosynthesis in Drosophila melanogaster." Molecular and cellular endocrinology 215.1...

  5. Antibodies 101: Isotypes

    Type
    Blog Post
    ...Garland Science; 2001. Structural variation in immunoglobulin constant regions. https://www.ncbi.nlm.nih....Science; 2001. The distribution and functions of immunoglobulin isotypes. Available from: https://www.ncbi....

  6. New Tool for Lineage Tracing: The ClonTracer Library

    Type
    Blog Post
    ...develop a way to quantify the take rate of mouse xenografts after cell implantation. Their goal was to figure...drug treatment in cell line and primary tumor xenograft models. She hopes that the library can be used...

  7. Adenoviral Vector Production and Troubleshooting

    Type
    Blog Post
    ...transgene expression (Interestingly, the strong immunogenicity of AdVs is what makes them ideal candidates...potential for the generation of RCA and reduced immunogenicity. In addition to E1/E3, non-structural genes...

  8. Antibodies 101: Choosing the Right Isotype

    Type
    Blog Post
    ...antibodies used for detection bind to non-target immunoglobulins naturally present in the tissue sample. There...antibody fragments to block the endogenous immunoglobulins and reduce the background staining but they...

  9. AAVs in Retinal Gene Therapy

    Type
    Blog Post
    ... abandoned early on due to its considerable immunogenicity and its transient expression. LV results in...to cause any disease in humans, and its low immunogenicity is critical when systemic delivery of high ...

  10. Plasmids 101: Choosing an Antibiotic Resistance Gene

    Type
    Blog Post
    ...Expensive.   Kanamycin Kanamycin, or kan, is an aminoglycoside which inhibits protein synthesis. The kan resistance...The NPTII gene confers resistance to several aminoglycosides, including G418 which is commonly used for ...

  11. Overcoming the AAV Size Limitation for CRISPR Delivery

    Type
    Blog Post
    ... for in vivo gene delivery due to their low immunogenicity and range of serotypes allowing preferential...Brötzmann J, Staahl BT, Taylor KL, Desmarais J, Nogales E, Doudna JA (2019) CasX enzymes comprise a distinct...

  12. Viral Production at Addgene

    Type
    Blog Post
    ...contamination in vector preparations can alter the immunogenic properties of the final product, particularly...plasmid purification protocol. To minimize the immunogenic properties of the final vector preparation, the...

  13. CRISPR Pooled gRNA Libraries

    Type
    Collection
    ...Neelamegham 3rd 10 3,637 Oxford Fly 64750 Knockout D. melanogaster Liu N/A 3 40,279 Pan-Druggable Cancer Library...

  16. Delivery Methods for Generating iPSCs

    Type
    Blog Post
    ...polycistronic cassette comprised of OCT4, SOX2, LIN28, NANOG, and a GFP reporter. Minicircles are removed from... plasmids (including reporters and RNAi) for:  NANOG  OCT4 SOX2 MYC KLF4 LIN28 Check out these plasmids...

  17. KLF Research Plasmids

    Type
    Collection
    ... metabolism, fibrosis, atherosclerosis, and carcinogenesis. The defining feature of the KLF family is ...

  18. Antibody Plasmid Collection

    Type
    Collection
    ...CRISPR system to rapidly engineer the constant immunoglobulin domains to obtain recombinant hybridomas, which...

  19. p53 Pathway

    Type
    Collection
    ... Serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 1 PERP TP53...
Showing: 21 - 40 of 96 results