...with the endogenous tRNA-processing system Plant Utilizes the tRNA processing system to reduce size and...Editing in Monocot and Dicot Plants Plant PCR-based procedure to rapidly generate multiple sgRNA expression ...Bullock pCFD5 Drosophila Utilizes endogenous tRNA processing machinery to excise multiple functional gRNAs...

...15574 GAL 25Q+ProCFPp303 HTT CFP GAL1 Huntington's Susan Lindquist 15575 GAL 103Q+ProCFPp303 HTT CFP GAL1...15578 GAL HA25QDProCFPp303 HTT HA, CFP GAL1 Huntington's Susan Lindquist 15579 GAL HA103QDProCFPp303 HTT HA... Huntington's Susan Lindquist 15570 GAL HA25Q+ProCFPp303 HTT HA, CFP GAL1 Huntington's Susan Lindquist...GAL1 Huntington's Susan Lindquist 15584 GAL FLAG25QDProCFPp303 HTT FLAG, CFP GAL1 Huntington's Susan Lindquist...Lindquist 15585 GAL FLAG46QDProCFPp303 HTT FLAG, CFP GAL1 Huntington's Susan Lindquist 15586 GAL FLAG72QDProCFPp303...GAL1 Huntington's Susan Lindquist 15589 MET FLAG46QDProCFPp303 HTT FLAG, CFP MET1 Huntington's Susan Lindquist...Lindquist 15590 MET FLAG72QDProCFPp303 HTT FLAG, CFP MET1 Huntington's Susan Lindquist 15591 MET FLAG103QDProCFPp303...

....1016/j.chembiol.2012.02.006. 2 Guntas et al., Proc Natl Acad Sci USA 2015 Jan 6; 112(1):112-7. doi:... the genome of your favorite cells. Ma et al., Proc Natl Acad Sci U S A. 2015 Mar 10;112(10):3002-7....precision in a native context. Chatterjee A et al. Proc Natl Acad Sci U S A. 2013 Jul 16;110(29):11803-8...creating a single-molecule reporter of intracellular processes. Multimerization via the SunTag system also shows...conditions, and could initiate specified cellular processes or be observed at a later time via DNA sequencing...Dimer (oLID) could be used to modulate biological processes, but it did not show large changes in binding ...from the laboratory of Ian Frew simplifies the process of creating complex polycistronic lentiviruses....

... our production process? At Addgene, we provide transparency in our production process ...

...Addgene collection? Click here to start the deposit process and have your plasmids added to the list. You can...more information and assistance with the deposit process. Want more information on the wide variety of Cas...

...binding between the template and PBS, increase processivity, and improve thermostability. PE3 uses the PE2...for repair during heteroduplex resolution. The process of double nicking, however, increases indel formation...

...also kindly provided this protocol detailing the process for packaging these transfer vectors and generating...pAdx-CMV-LacZ is uniquely suited to simplify the cloning process as it can be used in blue-white screening and forms...

... higher multiple circular DNA forms in bacteria. Proc Natl Acad Sci U S A. 1968 Dec;61(4):1406-13. doi...result of multiple plasmid copies combining in a process known as plasmid multimerization. Plasmid Multimerization...our plasmid samples during our quality control process. Since MiSeq involves sequence assembly from short...plasmid DNA by capillary gel electrophoresis. BioProcess Int. 2006 Sep;4(8):38-40. Summers D. Timing, ... burden seed-stock and fed-batch fermentation processes. Biotechnol Bioeng. 2009 Aug 15;103(6):1129-43...

...mitigation with cross-over insensitive ATG-out vectors. Proc Natl Acad Sci USA 116:27001–27010. https://doi.org...

...Reyes-Gutierrez P, Wolfe SA, Zhang S, Pederson T. Proc Natl Acad Sci U S A . 112(10):3002-7. PMID: 25713381...Many of these enzymes also have the ability to process crRNA precursors to mature crRNAs. Upon ssRNA recognition...is necessary to model your specific disease or process of interest. Do you want to: Generate complete ... method of delivery and the cell type. Before proceeding with your experiment, it may be necessary to ...

... present, the introns are excised during mRNA processing, producing a functional molecular tool (C,F)....Deisseroth Lab maintains a Standard Operating Procedure with general principles for working with INTRSECT...

...BRET-based in vivo imaging of multiple cellular processes, biosensors, or optogenetic tools . pcDNA3.1+-...used for screening of transformants in cloning process. Lynne Postovit 119760 pSGDlucV3.0 Firefly/Renilla...

...infection, the immune system triggers several processes to activate cells to recognize and get rid of ...attack invading pathogens. Innate immune cells also process and present foreign antigens to adaptive immune... have plasmids available for request. Antigen Processing and Presentation The cornerstone of the immune...ERp60, ERp61, GRP57, GRP58, HsT17083, P58, PI-PLC PROCR protein C receptor, endothelial (EPCR) CCCA, CCD41...represent over 25 cellular signaling pathways and processes. Addgene Blogs Adeno Associated Virus (AAV) for...

...opens in a new window) and an optimized sorting process. This combination allows IPI to generate antibodies...

...transcriptional regulators with diverse roles in processes such as cell proliferation, differentiation, inflammation...

...22745249 Sequence- and structure-specific RNA processing by a CRISPR endonuclease. Haurwitz RE, Jinek ...

...pSQT1601 encodes the Csy4 ribonuclease (required for processing of gRNAs co-expressed in vectors made from pSQT1313...

...biomolecules or other physiological intracellular processes with genetically encoded fluorescent biosensors...

...plasmids to your cart, then complete the checkout process online. Click here for additional ordering information...

... conditions. Viral Production : Our production process and quality control, with examples of quality control...