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Showing: 101 - 120 of 305 results

  1. Plasmids 101: Yeast Vectors

    Type
    Blog Post
    ...plasmids used in the lab typically contain all the necessary components to allow shuttling between E. coli ...transformation efficiency; however, this region does not necessarily promote replication.  Selection markers for yeast...

  3. Rosella: A Fluorescent pH-Biosensor for Studying Autophagy

    Type
    Blog Post
    ...targeted autophagy of mitochondria. As shown in Sargsyan et al. It is also possible to make Rosella fusions...doi:10.4161/auto.5331. PubMed PMID: 18094608 2. Sargsyan, A., Cai, J., Fandino, L. B., Labasky, M. E., ...

  4. Summer SciComm Series: Cognitive Load

    Type
    Blog Post
    ...surface, but it’s quite easy to add in “necessary” detail after “necessary” detail and end up completely overwhelming...

  6. Rabies and Neuronal Tracing

    Type
    Blog Post
    ...into viral genome, so viral progeny will not necessarily express EnvA unless it is supplied in trans. ...phenomenon has shown that the RABV G protein is necessary and sufficient for retrograde transmission (Etessami...

  7. Viral Vectors 101: Calcium Sensors

    Type
    Blog Post
    ...calcium binding sites per sensor (to decrease unnecessary calcium buffering and to increase sensitivity...great FRET sensor option if you have the setup necessary for readout.   We’ve got a live one ...

  11. 10 Basic tips for mammalian cell culture

    Type
    Blog Post
    ... have the power to take the added precautions necessary to keep your cells from becoming contaminated....you’re wearing before you start. Also, avoid unnecessary talking because talking generates microbe-laden...

  12. Viral Vectors 101: Voltage Indicators

    Type
    Blog Post
    ...with these sensors, but further optimization is necessary for these to be as effective at the single cell...perform poorly in two-photon conditions, which are necessary conditions for penetrating deeper tissues depths...

  13. Summer SciComm Series: Modes of Communication

    Type
    Blog Post
    ...content.  What are you interested in? Math, sarcasm, romance, and spacecraft! Image courtesy of xkcd.com... of a learning curve it will be to get to the necessary level  of proficiency.  What kind of content are...

  15. Protocol - How to Design Primers

    Type
    Protocol
    ...Primer Design for PCR Oligonucleotide primers are necessary when running a PCR reaction. One needs to design...

  16. AAV Titers: Where do they come from and what do they mean?

    Type
    Blog Post
    ...theoretical maximum value, physical titers don’t necessarily give any indication of the infectious titer. ...and convenient. However, these methods are not necessarily precise or accurate for quantifying AAV because...

  17. Transferable Skills Guide: Career Planning Resources

    Type
    Blog Post
    ..., though – someone accepting your CV doesn’t necessarily mean that you are a future employee of the company...might even be a colleague. A mentor does not necessarily need to be someone a lot older than you are; ...

  19. Plasmids 101: The Promoter Region – Let's Go!

    Type
    Blog Post
    ...resistance gene and the origin of replication are necessary to replicate plasmids and make sure the cells ... it. Here, we'll cover the promoter, which is necessary for getting the plasmid express our gene of interest...

  20. Plasmids 101: Gateway Cloning

    Type
    Blog Post
    ...an expression clone with all of the components necessary for gene expression.     The LR Reaction ...all kind of organisms from mammals to E. coli. Glossary of Gateway cloning vectors Vector type  Vector...
Showing: 101 - 120 of 305 results