...DiBerto JF, English JG, Glaudin AM, Krumm BE, Slocum ST, Che T, Gavin AC, McCorvy JD, Roth BL, Strachan RT...technique. Lastly, the activity of both antagonists and inverse-agonists could be measured using the TRUPATH...downstream complexes involved, it can be difficult for scientists to understand how GPCR activation leads to downstream...(Olsen RHJ et al., 2020). A transducer complex consists of a trimer of three subunits: alpha, beta, and...and understudied GPCRs in response to different agonists. These studies showed the promiscuity of GPCRs... transducers. The TRUPATH collection provides scientists with a toolkit to gain an understanding of the...blog Browse all of our fluorescent protein blog posts Find other fluorescent biosensors Resources on ...

...indicator: JEDI-2P  by: Brian O'Neill  The François St-Pierre lab and collaborators have successfully developed...imaging of voltages. This includes monitoring of fast bursts, as well as slow up/down voltage states in deep...

...CellTag from the Morris Lab at Washington University in St. Louis provides a combinatorial indexing strategy...as single cell RNA-seq (scRNA-seq), now allows scientists to identify the gene expression patterns unique... the direct reprogramming of mouse embryonic fibroblasts (MEFs) into induced endoderm progenitors (iEPs...

...Serotype Name 119036  AAV1  pAAV-hsyn-flex-Voltron-ST Chemogenetics Plasmid Serotype Name 75033...Addgene blog Read all of our viral vectors blog posts Find tips for preparing AAVs Download our viral ...

...Biological Studies The Scripps Research Institute St. Jude Children's Research Hospital  Stony Brook University...turnaround times: less than two days for material requests and less than one week for material deposits. ...they may seem like boilerplate drudgery to some scientists, MTAs are important—they guarantee certain rights...Stopwatch! Any organization that deposits with and/or requests materials from Addgene can qualify. We also reward...agreement just once, covering nearly all future requests/deposits. This reduces the processing time of ...Addgene, whose terms apply to all future materials requests from Addgene MTA: material transfer agreement ...

...Serotype Name 119036  AAV1  pAAV-hsyn-flex-Voltron-ST DREADDs / Chemogenetics AAV We have also expanded...easier for scientists, we now offer trial size 20 µL samples of control AAV that scientists can use for...control AAVs to our inventory since we know that scientists need these tools both for pilot experiments and...results of studies. When planning AAV experiments, scientists often have to choose the best serotype for their...Addgene blog Read all of our viral vectors blog posts Find tips for preparing AAVs Download our viral ...

... (NM) 3' NNNNGATT  Streptococcus thermophilus (ST) 3' NNAGAAW  Treponema denticola (TD) 3' NAAAAC...

...10.1016/j.cell.2020.12.015 Garcia-Beltran WF, Lam EC, St. Denis K, Nitido AD, Garcia ZH, Hauser BM, Feldman...research. “I hope that by sharing the plasmids other scientists would be able to expand upon the work and continue... Addgene blog Browse all COVID-19 related blog posts Learn more about pseudotyping Resources on Addgene.org...

...Court of Appeals. She served as in-house counsel at St. Elizabeth’s Hospital and was associated with Hale...scientific community manage those plasmids so scientists aren’t bogged down with distributing them and...

...science policy, and science and technology studies (STS). I also worked with a number of organizations in...blistering pace, it is becoming ever more urgent for scientists to engage broadly with diverse communities, to...about genetics and facilitate dialogue between scientists and communities. As the director of new initiatives...translating all these into social media and blog posts, lesson plans, or information briefs for policymakers...not experts in particular scientific fields. As scientists, many of us are used to being very focused on...research. At both a practical and ethical level, scientists arguably have a duty to engage the public on ...communication Find all of our science communication posts Extracurricular activities for a strong science ...

...and temporally target single cells. Now, Francois St-Pierre’s lab developed a new form of FACS-based cell... Scientists looking for more compact genome editors due to delivery constraints and scientists seeking...utilizing recombinases to manipulate genomes, scientists can control expression of their genes of interest...

...meningitidis (NM) 3' NNNNGATT Streptococcus thermophilus (ST) 3' NNAGAAW Treponema denticola (TD) 3' NAAAAC Additional...CRISPR-Display. 2015. Shechner DM, Hacisuleyman E, Younger ST, Rinn JL. Nat Methods . 12(7):664-70. PMID: 26030444...transcription-activator-like effector nucleases (TALENs) required scientists to design and generate a new nuclease pair for...sites throughout the genome where partial homology exists. These sites are called off-targets and need to...

...e7. Pereira Fibroblasts Sertoli Cells Lentiviral Mouse Direct Reprogramming of Fibroblasts into Embryonic.... Jaenisch Fibroblasts Hepatocytes Retroviral Mouse Direct conversion of mouse fibroblasts to hepatocyte-like...e28719. Gearhart Fibroblasts Neurons Lentiviral Mouse Direct conversion of fibroblasts to functional neurons...Wernig Fibroblasts Dopaminergic Neurons Lentiviral Human Direct conversion of human fibroblasts to dopaminergic... Parmar Fibroblasts Neurons Lentiviral Human MicroRNA-mediated conversion of human fibroblasts to neurons...Crabtree Fibroblasts Motor Neurons Retroviral Mouse/Human Conversion of mouse and human fibroblasts into ...Eggan Fibroblasts Neural Precursor Cells Lentiviral Mouse Direct conversion of mouse fibroblasts to self-renewing...

...biology professors at the University of Washington in St. Louis, offer guidance on how to make a low-cost ...about research. Videos can be shared by research scientists, faculty, students, private sector researchers... 6 Tools & Tips: Online Social Networking for Scientists Read about the Evolution of Scientific Impact...

...jejuni (Cj) NNNNRYAC Streptococcus thermophilus (St) NNAGAAW Treponema denticola (Td) NAAAAC ...

...-repressors or other transcriptional modifiers suggests the major function of MECP2 is to regulate gene... Material Publication PI N126I A377T N126I M Fibroblasts & iPSC (Link opens in a new window) PMID: 26944080...new window) Alysson Muotri Q83X C247T Q83X M Fibroblasts & iPSC (Link opens in a new window) PMID: 26944080...Alysson Muotri c.806delG 806delG G269Afs*20 M Fibroblasts & iPSC Unpublished (Link opens in a new window...window) Wendy Gold A140V C419T A140V M Fibroblasts Unpublished (Link opens in a new window) Wendy Gold AN_BU...AN_BU 808delC Arg270Glufs*19 F Fibroblasts & iPSC Unpublished (Link opens in a new window) Wendy Gold ...Gold BO_DI 806delG G269Afs*20 F Fibroblasts Unpublished (Link opens in a new window) Wendy Gold HO_AN G917A...

...elegans. WormBase - A consortium of biologists and computer scientists dedicated to providing the research...studies in cell biology, development, and aging. Scientists can knock down genes by feeding worms bacteria...transparent body of the C. elegans hermaphrodite consists of 959 somatic cells, which makes them attractive...Plasmids Expressing Worm Genes/Inserts The table below lists plasmids that contain a worm ( C. elegans, C. briggsae...elegans . Return to top Other Resources Addgene Blog Posts An “elegans” Approach to Better CRISPR/Cas9 Editing...

... CRISPR Protocols gRNA Design Tools CRISPR Blog Posts The CRISPR revolution shows no signs of slowing ...bacteriophages was not elucidated until 2007. Scientists hypothesized that prokaryotes used CRISPR as ...mammalian cell culture. Five years later, PubMed lists more than 6,300 CRISPR-related publications, many... it suitable for multiplexing and use in AAV. Scientists have also developed CRISPR editing technologies...it a very flexible system. Other Applications Scientists have also used the targeting capability of Cas9...editing risk must be made very low or nonexistent. Scientists continue to wrestle with the possibility of postnatal...check out Addgene’s CRISPR Guide and CRISPR blog posts , or find CRISPR plasmids for your research. Addgene...

...antibody binds tightly to a specific antigen, scientists can use it to pull out a protein of choice from... to improve binding and reproducibility. Some scientists choose to work with full length recombinant monoclonal...culture media and can be collected for use. Other scientists may choose to work with a smaller fragment of...antibodies (also known as nanobodies). For example, scientists often use plasmids encoding scFv fragments that...

... ). The Bogdanove group also hosts web-based software to help scientists find potential TAL effector targets...transcription activator-like (TAL) effectors, scientists in several biological disciplines are coming ...domains to a specific DNA sequence ( Figure 1A ). Scientists have identified a large number of zinc fingers...