...overnight. For diagnostic digests, 1-2 hours is often sufficient. For digests with >1 µg of DNA used for...hundreds of different restriction enzymes, allowing scientists to target a wide variety of recognition sequences...

...transfection in individual harvests or a combined harvest where all the individual harvests are pooled. If pooling...pooling harvests, transfer the harvested media to a polypropylene storage tube and store at 4 °C between...

...catalyze chemical reactions such as restriction digests needed in molecular biology, and thaw cell lines... different types of water baths that exist as scientists work with tubes and bottles of different sizes...

...recognize, restriction digests have become the most widely used method scientists employ to selectively...

...Video Link Description Related Page MTA Guide - Requests Your guide to the MTA process when requesting ...international collaboration, and offers career advice for scientists looking to expand their international reach. ...

...the Whitehead Institute for Biomedical Research hosts an siRNA Selection Program that can be accessed ...reaction buffer selection when setting up double digests. Digest pLKO.1 TRC-cloning vector with AgeI. Mix...Information Addgene is committed to providing scientists with high-quality goods and services. Addgene...

...method for the assembly of plasmids, and molecular biologists now use this method extensively. Why Gibson Cloning... Assembly master mix (isothermal reaction mix) consists of three different enzymes within a single buffer...

... Procedure Digest your DNA: Set up restriction digests for your donor and recipient plasmids. Because ...each plasmid to help with troubleshooting if your digests don’t look as you expected. Once you have cut out...

...digestion. Digest your DNA: Set up restriction digests for your PCR product and recipient plasmid. Because...each vector to help with troubleshooting if your digests don’t look as you expected. When cloning by PCR...

...your feet. *Pro-Tip* If they are able, sometimes scientists work for a long time while standing. Wear comfortable...

...used by Addgene during quality control? Addgene lists the primers used to obtain each result above the...

...concentration with a spectrophotometer Restriction Digests Cut and analyze plasmid DNA with restriction enzymes...

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...that were generated by EcoRI digestion: Usually, scientists select two different enzymes for adding an insert...

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...gene for use as a selectable marker in bacteria. Scientists have made many genetic modifications to create...

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...encounter: the compound light microscope. As the name suggests, light microscopes take advantage of the physical...

...the proper way to pipette, it’s important for scientists to calibrate their pipettes to make sure that...