...mGold Francois St-Pierre 158009 pCMV-mGold-Actin-C-18 Actin Filaments Actin mGold Francois St-Pierre 49149...mGold Francois St-Pierre 158002 pCMV-mGold-Rab4a-C-7 Endosomes Rab4a mGold Francois St-Pierre 14873 GolgiCFP...pCMV-mGold-CAF1-C-10 Nucleus CAF-1 mGold Francois St-Pierre 27705 CAV1-mCherry Caveolae Cav1 mCherry Ari...-mGold-Mito-N-7 Mitochondria Cox8A mGold Francois St-Pierre 49151 mito-BFP Mitochondria mitochondrial ...pCaggs-NLS-PAmCherry1-GSS-EGFP Nucleus NLS mGold Francois St-Pierre 14893 pEBFP2-Nuc Nucleus NLS EBFP2 Robert ...pCMV-mGold-MannII-N-10 Golgi apparatus MannII mGold Francois St-Pierre 85050 pmScarlet-i_Giantin_C1 Golgi apparatus...pCMV-mGold-Lysosome-N-20 Lysosome Lamp1 mGold Francois St-Pierre 98882 Lamp1-mNeonGreen Lysosomes Lamp1 mNeonGreen...

...-Voltron-ST Syn Voltron-ST none Cre dependent 1 Schreiter 172907 pGP-AAV-syn-FLEX-Volton2-ST-WPRE Syn ...dependent 1 St-Pierre 179460 pAAV-EF1a-DIO-JEDI-2P-WPRE EF1a JEDI-2P none Cre dependent 1 St-Pierre 179463...Constitutive 9 St-Pierre 179464 pAAV-hSyn-JEDI-2P-WPRE Syn JEDI-2P none Constitutive 9 St-Pierre 5-HT Sensors...GENIE 174007 pAAV-hSyn-DIO-jGCaMP8s-P2A-ChrimsonR-ST Syn jGCaMP8s none Cre-dependent 9 Histed 176749 AAV-Syn-H2B-jGCaMP8s-WPRE...Syn Voltron2-ST none Cre dependent 1 GENIE Voltage Reporter: JEDI-2P 179459 pAAV-EF1a-DIO-JEDI-2P-Kv-WPRE...

... Deisseroth 105448 pAAV-hSyn-DIO-ChrimsonR-mRuby2-ST Syn ChrimsonR (soma-targeted) mRuby2 Cre dependent...Cre dependent 9 Harvey 108912 pAAV-CAG-DIO-ChroME-ST-P2A-H2B-mRuby3 CAG ChroME (soma-targeted) mRuby3 ...Jensen 174007 pAAV-hSyn-DIO-jGCaMP8s-P2A-ChrimsonR-ST Syn ChrimsonR (soma-targeted) GCaMP8s Cre dependent...Yizhar 109048 pAAV-CAG-DIO-NLS-mRuby3-IRES-eGtACR1-ST CAG eGtACR1 (soma-targeted) mRuby3 Cre dependent ...

...Gradinaru 105448-AAV9 pAAV-hSyn-DIO-ChrimsonR-mRuby2-ST Cation channelrhodopsin ChrimsonR fused to mRuby2...expression Edward Boyden 108912-AAV9 pAAV-CAG-DIO-ChroME-ST-P2A-H2B-mRuby3 Cation channelrhodopsin ChroME targeted... 174007-AAV9 pAAV-hSyn-DIO-jGCaMP8s-P2A-ChrimsonR-ST Cre dependent co-expression of jGCaMP8s and soma-...expressed under the mammalian promoter (EF1a) Francois St-Pierre 179460-AAV1 pAAV-EF1a-DIO-JEDI-2P-WPRE Double...control of the mammalian promoter (EF1a) Francois St-Pierre Antibodies Addgene distributes ready-to-use...

...integration of pLC97 can be found in this article: St-Pierre et al., One-Step Cloning and Chromosomal Integration... CRISPR Protocols gRNA Design Tools CRISPR Blog Posts The David Bikard Lab at the Institute Pasteur has...

...21, 2018, 9 AM - 5 PM Location: Addgene, 75 Sidney St, Cambridge, MA, 02139 Organizing Committee Chair:... be a publicly shared practical guide for all scientists in the field and every attendee of the meeting...on this communication and you will be helping scientists successfully adopt these tools. Thank you for...

...whole series of blog posts in one pdf - download the Addgene Management for Scientists Ebook.  Fire, Snowball...the fifth in a 5 part series on Management for scientists.  We are inundated with articles and books on...coordination and can require technical mastery. Scientists need to be doers for a long time before they ...master the skills they need to be successful. Scientists are lifelong learners so we are all likely spending...about being an effective manager, read the other posts in this series. Listen to our podcast interview ... primary role of doer for your entire career. Scientists train as doers and often maintain an enjoyment...Richard Badham Leadership Insights for Engineers, Scientists and Computer Professionals as Leaders, by Ken...

...the fourth in a 5 part series on Management for scientists. Once you are responsible for managing others...Addgene Blog Read the Rest of the Management for Scientists Series Learn How Volunteering at a Professional...  management for scientists...

...the third in a 5 part series on Management for scientists. Feedback is help and it should result in authentic...nonthreatening way. However, I have also found that scientists seem to find it easier to give feedback in formal...the third in a 5 part series on Management for Scientists. Subscribe to the Addgene Blog to follow the ... management for scientists...

... Management for scientists. This second installment in the Management for Scientists series will focus...series on Introduction to Managing People for Scientists. Subscribe to the Addgene Blog to follow the ... management for scientists...

... career pathways exist for scientists and engineers, a few brave scientists will choose to start their...gels ran.” Dr. Ellis tries to lead his team as a scientists and a friend. George Church runs his lab fairly... offers some great resources for Early Career Scientists, including their "Making the Right Moves: A Practical...taking the time to speak with us about adding new scientists to their labs. Further Reading & Resources ...Leadership Courses HHMI Resources for Early Career Scientists Science Careers: Running Your Lab (pdf) Advice...

...don’t say. Scientists that are having a good experience will be quick to say so, but scientists that are..., for this second entry in the “Mentoring for Scientists Series”, let’s focus on how to choose an advisor...standards and interact professionally with other scientists. The specific research area is not as important...Personality and style conflicts are a common reason for scientists to change labs – do as much as you can to ensure...ResearchGate and LinkedIn to find past lab members. Most scientists love to talk about their past lab experiences...concerns you, don’t just brush this away. Many scientists think, “Oh, but that won’t happen to me – I am...developing your career in science.  Read other posts in our Science Mentors series: Part 1: "What Makes...

...Breakdown of Addgene's top blog posts from 2015 Our most popular posts continue to be those from our Plasmids...year for the Addgene blog. We’ve released over 80 posts on a diverse array of topics pertinent to the research...research community, two eBooks (Management for Scientists and the Plasmids 101 2nd ed, the 3rd edition ...community, we’ve curated a list of our 10 top-viewed posts from 2015 and encourage you to give them a read ... Plasmids 101 and CRISPR 101 series. These posts aim to give researchers the background they need to get... incredibly happy to see that one of our career posts has made it to the top of the list this year. While... goals and believe the popularity of our career posts shows that we are doing just that. Do you like the...

...ability to, some communities are looking for bench scientists to assist in labs that are testing for COVID-...something like puzzling or crafts, while listening to podcasts made for kids. Plan out meals and snacks ahead...

...gratitude on the blog by highlighting some of the great posts written by Addgene depositors. Below you’ll find...find brief summaries of several excellent guest posts from our depositors. Interested in writing your own...

...classic parametric tests and one of the simplest statistical tests available. Tailed t-tests If you are solely...needs to be accounted for. ANOVA tests ANOVA tests are essentially t-tests with room for more variables and...distribution profiles.   Parametric tests  Statistical tests fall into two general categories: parametric... blog we will be discussing parametric tests. Parametric tests are suitable for data sets that “play by...which we will discuss more in another blog. T-tests T-tests compare the mean of two groups to determine ...statistical test   Post hoc tests Knowing that significance exists somewhere amongst multiple groups...performed first, and then post hoc tests can be run on these tests. All sample comparisons Do you really...

...700 members and hosts a range of scientific meetings for students to attend. It also hosts high level academic... students to network, find groups with common interests, and settle into university life. The fairs are...students. Freshers fairs are also where many future scientists are introduced to the biology and chemistry societies...public. The society organises talks by prominent scientists, including Nobel Laureates, who speak about their...networking meetups. The society hopes to help young scientists interested in this field excel and develop their...inside and outside academia. Inspiring future scientists At Addgene, we’re incredibly glad to see these...very important to develop a network of fellow scientists that you can grow with, learn from, and mentor...

...blog this year. Spoiler alert: many of the top 10 posts from this year were influenced by COVID-19 and our...new cloning grade DNA service. We've had 11 blog posts on COVID-19 featuring plasmid tools from depositors...transfer offices to make reagents available to scientists in both academia and industry. This year, we ...further ado, here are the top ten! Top 10 blog posts from 2020 10. What's the Best Way to Elute and Store...synthetic biology. 7. 25 Work From Home Tips for Scientists by Jennifer Tsang These tips were written early...

...during 2014. We were excited to have more and more scientists offering to share their stories – about their... I’ve compiled a list of the Top 10 Most Viewed Posts on our blog from the past year. A quick glance shows...technique has developed and improved. The CRISPR posts written by our guest bloggers, the experts in the...CRISPR/Cas field, have been extremely helpful for scientists that are looking for more detailed information...technology. Our Plasmids 101 series continues to grab scientists’ attention, and as we move into next year we’...many additional questions. We’re happy to see scientists making their own cells to save money and create.... If you haven’t yet read the following Top 10 posts of 2014, take some time and see what you’re missing...

... If you have been following the posts in this Mentoring for Scientists series, you have: realized the ...Mentoring for Scientists" ebook, which includes the entire series of mentoring blog posts, the "Forced ... final post in the Addgene Blog Mentoring for Scientists Series. The entire series and additional resources...example and you can find it in our Mentoring for Scientists Guide.  You can use it to clarify and discuss...