...mGold Francois St-Pierre 158009 pCMV-mGold-Actin-C-18 Actin Filaments Actin mGold Francois St-Pierre 49149...mGold Francois St-Pierre 158002 pCMV-mGold-Rab4a-C-7 Endosomes Rab4a mGold Francois St-Pierre 14873 GolgiCFP...pCMV-mGold-CAF1-C-10 Nucleus CAF-1 mGold Francois St-Pierre 27705 CAV1-mCherry Caveolae Cav1 mCherry Ari...-mGold-Mito-N-7 Mitochondria Cox8A mGold Francois St-Pierre 49151 mito-BFP Mitochondria mitochondrial ...pCaggs-NLS-PAmCherry1-GSS-EGFP Nucleus NLS mGold Francois St-Pierre 14893 pEBFP2-Nuc Nucleus NLS EBFP2 Robert ...pCMV-mGold-MannII-N-10 Golgi apparatus MannII mGold Francois St-Pierre 85050 pmScarlet-i_Giantin_C1 Golgi apparatus...pCMV-mGold-Lysosome-N-20 Lysosome Lamp1 mGold Francois St-Pierre 98882 Lamp1-mNeonGreen Lysosomes Lamp1 mNeonGreen...

...-Voltron-ST Syn Voltron-ST none Cre dependent 1 Schreiter 172907 pGP-AAV-syn-FLEX-Volton2-ST-WPRE Syn ...dependent 1 St-Pierre 179460 pAAV-EF1a-DIO-JEDI-2P-WPRE EF1a JEDI-2P none Cre dependent 1 St-Pierre 179463...Constitutive 9 St-Pierre 179464 pAAV-hSyn-JEDI-2P-WPRE Syn JEDI-2P none Constitutive 9 St-Pierre 5-HT Sensors...GENIE 174007 pAAV-hSyn-DIO-jGCaMP8s-P2A-ChrimsonR-ST Syn jGCaMP8s none Cre-dependent 9 Histed 176749 AAV-Syn-H2B-jGCaMP8s-WPRE...Syn Voltron2-ST none Cre dependent 1 GENIE Voltage Reporter: JEDI-2P 179459 pAAV-EF1a-DIO-JEDI-2P-Kv-WPRE...

... Deisseroth 105448 pAAV-hSyn-DIO-ChrimsonR-mRuby2-ST Syn ChrimsonR (soma-targeted) mRuby2 Cre dependent...Cre dependent 9 Harvey 108912 pAAV-CAG-DIO-ChroME-ST-P2A-H2B-mRuby3 CAG ChroME (soma-targeted) mRuby3 ...Jensen 174007 pAAV-hSyn-DIO-jGCaMP8s-P2A-ChrimsonR-ST Syn ChrimsonR (soma-targeted) GCaMP8s Cre dependent...Yizhar 109048 pAAV-CAG-DIO-NLS-mRuby3-IRES-eGtACR1-ST CAG eGtACR1 (soma-targeted) mRuby3 Cre dependent ...

...Gradinaru 105448-AAV9 pAAV-hSyn-DIO-ChrimsonR-mRuby2-ST Cation channelrhodopsin ChrimsonR fused to mRuby2...expression Edward Boyden 108912-AAV9 pAAV-CAG-DIO-ChroME-ST-P2A-H2B-mRuby3 Cation channelrhodopsin ChroME targeted... 174007-AAV9 pAAV-hSyn-DIO-jGCaMP8s-P2A-ChrimsonR-ST Cre dependent co-expression of jGCaMP8s and soma-...expressed under the mammalian promoter (EF1a) Francois St-Pierre 179460-AAV1 pAAV-EF1a-DIO-JEDI-2P-WPRE Double...control of the mammalian promoter (EF1a) Francois St-Pierre Antibodies Addgene distributes ready-to-use...

...integration of pLC97 can be found in this article: St-Pierre et al., One-Step Cloning and Chromosomal Integration... CRISPR Protocols gRNA Design Tools CRISPR Blog Posts The David Bikard Lab at the Institute Pasteur has...

...21, 2018, 9 AM - 5 PM Location: Addgene, 75 Sidney St, Cambridge, MA, 02139 Organizing Committee Chair:... be a publicly shared practical guide for all scientists in the field and every attendee of the meeting...on this communication and you will be helping scientists successfully adopt these tools. Thank you for...

...2022 Aug 16. pii: S0092-8674(22)00916-3. Francois St-Pierre Voltage Voltage Sensitive Fluorescent Protein...;75(5):779-85. Vincent Pieribone Voltage ArcLight-ST to detect subthreshold membrane potential changes...Photosynthesis-dependent H 2 O 2 transfer from chloroplasts to nuclei provides a high-light signalling mechanism...

...meningitidis (NM) 3' NNNNGATT Streptococcus thermophilus (ST) 3' NNAGAAW Treponema denticola (TD) 3' NAAAAC Additional...CRISPR-Display. 2015. Shechner DM, Hacisuleyman E, Younger ST, Rinn JL. Nat Methods . 12(7):664-70. PMID: 26030444...transcription-activator-like effector nucleases (TALENs) required scientists to design and generate a new nuclease pair for...sites throughout the genome where partial homology exists. These sites are called off-targets and need to...

...e7. Pereira Fibroblasts Sertoli Cells Lentiviral Mouse Direct Reprogramming of Fibroblasts into Embryonic.... Jaenisch Fibroblasts Hepatocytes Retroviral Mouse Direct conversion of mouse fibroblasts to hepatocyte-like...e28719. Gearhart Fibroblasts Neurons Lentiviral Mouse Direct conversion of fibroblasts to functional neurons...Wernig Fibroblasts Dopaminergic Neurons Lentiviral Human Direct conversion of human fibroblasts to dopaminergic... Parmar Fibroblasts Neurons Lentiviral Human MicroRNA-mediated conversion of human fibroblasts to neurons...Crabtree Fibroblasts Motor Neurons Retroviral Mouse/Human Conversion of mouse and human fibroblasts into ...Eggan Fibroblasts Neural Precursor Cells Lentiviral Mouse Direct conversion of mouse fibroblasts to self-renewing...

...-repressors or other transcriptional modifiers suggests the major function of MECP2 is to regulate gene... Material Publication PI N126I A377T N126I M Fibroblasts & iPSC (Link opens in a new window) PMID: 26944080...new window) Alysson Muotri Q83X C247T Q83X M Fibroblasts & iPSC (Link opens in a new window) PMID: 26944080...Alysson Muotri c.806delG 806delG G269Afs*20 M Fibroblasts & iPSC Unpublished (Link opens in a new window...window) Wendy Gold A140V C419T A140V M Fibroblasts Unpublished (Link opens in a new window) Wendy Gold AN_BU...AN_BU 808delC Arg270Glufs*19 F Fibroblasts & iPSC Unpublished (Link opens in a new window) Wendy Gold ...Gold BO_DI 806delG G269Afs*20 F Fibroblasts Unpublished (Link opens in a new window) Wendy Gold HO_AN G917A...

...elegans. WormBase - A consortium of biologists and computer scientists dedicated to providing the research...studies in cell biology, development, and aging. Scientists can knock down genes by feeding worms bacteria...transparent body of the C. elegans hermaphrodite consists of 959 somatic cells, which makes them attractive...Plasmids Expressing Worm Genes/Inserts The table below lists plasmids that contain a worm ( C. elegans, C. briggsae...elegans . Return to top Other Resources Addgene Blog Posts An “elegans” Approach to Better CRISPR/Cas9 Editing...

... CRISPR Protocols gRNA Design Tools CRISPR Blog Posts The CRISPR revolution shows no signs of slowing ...bacteriophages was not elucidated until 2007. Scientists hypothesized that prokaryotes used CRISPR as ...mammalian cell culture. Five years later, PubMed lists more than 6,300 CRISPR-related publications, many... it suitable for multiplexing and use in AAV. Scientists have also developed CRISPR editing technologies...it a very flexible system. Other Applications Scientists have also used the targeting capability of Cas9...editing risk must be made very low or nonexistent. Scientists continue to wrestle with the possibility of postnatal...check out Addgene’s CRISPR Guide and CRISPR blog posts , or find CRISPR plasmids for your research. Addgene...

...antibody binds tightly to a specific antigen, scientists can use it to pull out a protein of choice from... to improve binding and reproducibility. Some scientists choose to work with full length recombinant monoclonal...culture media and can be collected for use. Other scientists may choose to work with a smaller fragment of...antibodies (also known as nanobodies). For example, scientists often use plasmids encoding scFv fragments that...

... ). The Bogdanove group also hosts web-based software to help scientists find potential TAL effector targets...transcription activator-like (TAL) effectors, scientists in several biological disciplines are coming ...domains to a specific DNA sequence ( Figure 1A ). Scientists have identified a large number of zinc fingers...

... a fluorescent protein as a scaffold to allow scientists to selectively modulate gene expression....modulate biological activity. Early tools allow scientists to regulate transcription, and additional tools...other activities. By using GFP as a scaffold, scientists can design de novo systems using modular components...

...depositing and recipient scientists to further develop and refine these application lists. The plasmids we use... Protocols Recipient Instructions Antibody Blog Posts Addgene Help Center...

...Protocols gRNA Design Tools CRISPR Blog Posts The table below lists gRNA plasmids designed to target various...gRNA Plasmids Please note that the following table lists both published and unpublished plasmids containing...

...Search to find plasmids available to industry scientists while also adding additional filters such as ...plasmids that are currently available to industry scientists. ID Plasmid Gene/Insert PI Antibodies Addgene...what you would like to see available to industry scientists. Please note this does not guarantee availability...

...Addgene have been produced in-house by Addgene scientists. They are generated using standard methods individually...recommend repeated freeze thaw of antibodies and suggests preparing single-use aliquots before storing at... Protocols Recipient Instructions Antibody Blog Posts Addgene Help Center...

...speed progress towards curing and treating them. Scientists are studying protein aggregation, learning about...or way to prevent progression of PD. Treatment consists mainly of managing symptoms through medication...Factors Find plasmids used to transform iPSCs and fibroblasts into neurons and more. New and Noteworthy: Study...