... shown to be useful tools for light-mediated subcellular localization in mammalian cell culture and reversible...were used in a variety of experiments including subcellular visualization of 6 different organelles, a cell...dynamics. These fusion proteins have been used as subcellular markers to visualize the dynamics and interactions...proteins can act as markers for visualization of subcellular structure, for performing live-cell imaging experiments...

...secondary antibodies generated against isotype subclasses, such as mouse IgG2 or IgG4. This can be important...secondary antibodies which recognize specific isotype subclasses of primary antibodies can increase the possible...

... cells (e.g., a neuron subtype) and specific subcellular compartments for measurements. All of the sensors...Localization The calcium concentrations in different subcellular compartments can vary greatly. Additionally, ...

...lysosome-associated membrane protein, and following its subcellular localization overtime (Subach et al.) Monitor...particular biological event (trafficking to a subcellular location, start of gene expression, development...

... be included in a single PCR, for example for subcloning genes from different templates. All PCRs are ...can be included at the 5’ end of either primer. Subcloning - To move a gene from one vector to another simply...

...eukaryotic) proteins localize to chemically distinct subcellular environments, including the organelles that compose...focused on optimizing fluorescent proteins for subcellular environment. Currently, she is a SPIRE postdoctoral...

...microtubule-destabilizing drugs are impossible to target to specific subcellular regions, laser ablation can damage other cellular...reversibly control Rho GTPase activity on a global to subcellular scale, enabling precise control over vascular...

...electron microscopy (EM) tag providing superior subcellular localization of tagged proteins compared to traditional...proteins of interest for EM imaging, or targeted to subcellular regions or protein complexes for proteomic mapping...

...(CAG promoter) ‐ OR ‐ shRNA (H1 promoter when subcloned from pLVTHM) On Patrick Aebischer and Didier Trono...(CAG promoter) ‐ OR ‐ shRNA (H1 promoter when subcloned from pLVTHM) Off Patrick Aebischer and Didier ...1alpha promoter) ‐ OR ‐ shRNA (H1 promoter when subcloned from pLVTHM) On Patrick Aebischer and Didier Trono...hPGK promoter) ‐ OR ‐ shRNA (H1 promoter when subcloned from pLVTHM) On Patrick Aebischer and Didier Trono...hPGK promoter) ‐ OR ‐ shRNA (H1 promoter when subcloned from pLVTHM) Off Patrick Aebischer and Didier ...hUbiquitin promoter) ‐ OR ‐ shRNA (H1 promoter when subcloned from pLVTHM) On Patrick Aebischer and Didier Trono...hPrion promoter) ‐ OR ‐ shRNA (H1 promoter when subcloned from pLVTHM) On Patrick Aebischer and Didier Trono...

...2021). Enhancer viruses for combinatorial cell-subclass-specific labeling. Neuron,109(9), 1449–1464.e13... P. (2021). Functional enhancer elements drive subclass-selective expression from mouse to primate neocortex...

...Plasmids Find Plasmids for FRET Find Plasmids for Subcellular Localization ...

... used to measure kinase activity in specific subcellular compartments. Second, because KTRs shuttle between...

... are you’ve used a GFP derivative to conduct subcellular localization studies or make a reporter construct...

...doing some research, notably determining the subcellular localization and the number of disulfide bonds...

...antibodies can be isotype-specific or even isotype subclass-specific. This specificity can be used to ensure...Isotype Description Configuration and Valency Subclasses IgA Prevalent antibody in secretions (e.g. tears...should never be stored below 4 °C. Certain isotype subclasses, such as IgG3, aggregate after thawing and therefore...different species of origin, isotypes, and isotype subclasses of a primary antibody, which becomes the limiting...? You may need to buy isotype-specific or even subclass-specific secondary antibodies to allow for differentiation...

...Addgene Blog: Biosensors Luciferase Plasmids Subcellular Localization Optogenetics Plasmids Viral Service...blue/green) Imaging intraorganellar Ca(2+) at subcellular resolution using CEPIA. Nat Commun. 2014 Jun ...genetically encoded sensors for population and subcellular imaging of cAMP in vivo. Nat Methods. 2022 Nov...FRET-based cGMP biosensor SponGee: A Genetic Tool for Subcellular and Cell-Specific cGMP Manipulation. Cell Rep...for mitochondria or cytosol Hypoxia triggers subcellular compartmental redox signaling in vascular smooth... Ultrafast fluorescent voltage sensor ASAP2f Subcellular Imaging of Voltage and Calcium Signals Reveals...

...receptors are specific to isotype class or even subclass, and are part of the reason why different isotypes...

...targeted to specific tissues, cell types or even subcellular regions of a neuron. Optogenetic approaches excel...

...53190 pLV hUbC-Cas9-T2A-GFP : Co-expresses human optimized S. pyogenes Cas9 and GFP. 53191 pLV hUbC-dCas9-...human optimized S. pyogenes dCas9 and GFP. 53192 pLV hUbC-dCas9 VP64-T2A-GFP : Co-expresses human optimized...

...Engebrecht J, Trimmer JS (2019) A toolbox of IgG subclass-switched recombinant monoclonal antibodies for...