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We narrowed to 570 results for: RON

Showing: 361 - 400 of 570 results

  1. Transferable Skills Guide: Career Planning Resources

    Type
    Blog Post
    Published
    Nov. 14, 2017, 2 p.m.
    ...school and beyond is to live in your lab, right? Wrong! While it is clearly a good idea to be diligent ...them for work. Addgene’s very own Joanne Kamens, a strong advocate for career planning, has written a great...

  2. Tips for CRISPR Gene Editing in Mice

    Type
    Blog Post
    Published
    June 28, 2016, 10:59 a.m.
    ...however, if the microinjection was done as the pronuclei were fusing, you may have several mosaic mice....Daisuke, et al. "Generation of mutant mice by pronuclear injection of circular plasmid expressing Cas9...

  3. The Challenges of Cell Culture

    Type
    Blog Post
    Published
    May 5, 2016, 2:30 p.m.
    ...system could be as simple as a piece of paper on the front of the incubator containing the passage number, ...ensure the results are reliable. With this two-pronged approach, the many mistakes of the past can be ...

  4. Data Freedom: The Expansion of Data Sharing in Research Publications

    Type
    Blog Post
    Published
    Aug. 5, 2014, 6:51 p.m.
    ...onto data longer before publishing. But there are strong incentives to publish such as precedence, recognition...Lunenfeld-Tanenbaum Research Institute at Mount Sinai Hospital, Toronto, Canada where he studies protein-serine/threonine...

  5. Plasmids 101: Expressing shRNAs for RNA Interference

    Type
    Blog Post
    Published
    June 17, 2025, 1:15 p.m.
    ...common promoters are U6, H1, and 7SK. These are strong promoters with no tissue specificity, so in some...biogenesis, mechanisms of actions, and circulation. Frontiers in Endocrinology, 9. https://doi.org/10.3389/fendo...

  6. Antibodies 101: Introduction to Gating in Flow Cytometry

    Type
    Blog Post
    Published
    April 4, 2024, 1:15 p.m.
    ... Many cell types can be found in the tumor microenvironment (tumor cells, immune cells, blood cells and...recorded events) based on their expected size. The strong signals close to the graph’s origin mainly stem...

  7. New Optogenetic Tools for Cytoskeleton and Membrane Control

    Type
    Blog Post
    Published
    Sept. 28, 2023, 1:15 p.m.
    ...light-sensitive ion channels used to control activities like neuronal signaling — think of a mouse with an LED brain... — but there’s a lot more to optogenetics than neuronal activity. Researchers have designed a variety ...

  8. Hot Biosensors 2022: Year-End Roundup

    Type
    Blog Post
    Published
    Dec. 6, 2022, 2:15 p.m.
    ...MTRIA sensors will be useful for investigating neuronal signaling and for engineering improved sensors...Small Near-Infrared Fluorescent Calcium Indicator. Front Cell Dev Biol. 2022 Jun 29;10:880107. doi: 10.3389...

  10. 3 Tips to Improve HDR Efficiency for CRISPR Editing in Human Cells

    Type
    Blog Post
    Published
    Sept. 5, 2017, 1:58 p.m.
    ... cells affected by the disease and study what’s wrong with the cells. However, our excitement turned into...picking 100 versus 10,000 clones! We would therefore strongly encourage any researcher to incorporate CRISPR...

  11. Deep Mutational Scanning with One Pot Saturation Mutagenesis

    Type
    Blog Post
    Published
    Feb. 22, 2017, 3:30 p.m.
    ...compared to one pot saturation mutagenesis. Error-prone PCR: This method amplifies the wild-type template...Chaput, J. C. (2010). “Random Mutagenesis by Error-Prone PCR”. Methods in Molecular Biology In Vitro Mutagenesis...

  12. CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9

    Type
    Blog Post
    Published
    Oct. 11, 2016, 2:30 p.m.
    ...Yiping Qi lab and the MoClo Plant Parts Kit from the Patron Lab.  As with all plasmids in the repository, we...Genome-Editing Tool: Application in Improvement of Crops. Front Plant Sci 7: . https://doi.org/10.3389/fpls.2016.00506...

  13. 10 Steps to a Perfect Science Talk

    Type
    Blog Post
    Published
    Aug. 23, 2016, 2:30 p.m.
    ...Joanne's Reddit AMA 6. Practice, practice, practice in front of a real audience Good, even great, speaking skills...help you avoid giving only yes or no answers in front of a crowd. Having answer slides ready after your...

  14. Treating Muscular Dystrophy with CRISPR Gene Editing

    Type
    Blog Post
    Published
    Jan. 26, 2016, 3:30 p.m.
    ...restore the dystrophin reading frame. gRNAs bind in intronic regions, so low-frequency indels will not cause...exonic regions comes with much more risk than the intronic editing used in the exon skipping approaches here...

  15. How to Negotiate a Successful Lab Start Up Budget

    Type
    Blog Post
    Published
    Sept. 1, 2015, 2:30 p.m.
    ...excessive costs in your lab. That in itself is a strong negotiating tactic, and puts you ahead of most ... create productive and manageable laboratory environments. His business skills enhance his passion for...

  16. Is this the right place for me? 8 tactics for choosing a lab

    Type
    Blog Post
    Published
    Oct. 2, 2018, 12:56 p.m.
    ...reputation? If you are choosing a training lab, I strongly suggest you complete a work rotation in the lab...and non-academic career paths. I can’t say this strongly enough - don’t choose a mentor that sees you only...

  17. How to Design Your gRNA for CRISPR Genome Editing

    Type
    Blog Post
    Published
    Sept. 24, 2020, 1:15 p.m.
    ...mediated dsDNA breaks: following a cut, the error-prone nature of non-homologous end joining (NHEJ) often...optimized sequence will do little if it is in the wrong place, but because the target window is more-narrow...

  19. Generating Mouse Models Using CRISPR/Cas9

    Type
    Blog Post
    Published
    July 12, 2016, 2:30 p.m.
    ...brought together and microinjected into either the pronucleus or the cytoplasm of fertilized mouse eggs. Or...remember that you are working with RNAs, which are prone to degradation by the omnipresent RNase. You may...

  20. Live and Let Dye: Self-Labeling Protein Tags

    Type
    Blog Post
    Published
    April 16, 2024, 1:15 p.m.
    ...the labeling kinetics or the fluorophore’s microenvironment to enhance brightness, stability, or other...10.1021/ar200099f Keppler, A., Gendreizig, S., Gronemeyer, T., Pick, H., Vogel, H., & Johnsson, K. (2003...

  21. Finding Your Science Policy Path

    Type
    Blog Post
    Published
    Sept. 22, 2020, 1:15 p.m.
    ...backgrounds with technical expertise that ranges from astronomy to bioethics to chemical engineering and everything...policy training programs and fellowships have a strong focus on early career researchers who have decided...

  22. Typing CRISPR Systems

    Type
    Blog Post
    Published
    March 18, 2025, 1:15 p.m.
    ...extreme sequence divergence that requires a multi-pronged analysis to sort effectively. The current classification... functions within Class 1 CRISPR-CAS systems. Frontiers in Microbiology, 12. https://doi.org/10.3389/fmicb...

  23. Plasmids 101: Modular Cloning Applications and Kits

    Type
    Blog Post
    Published
    May 14, 2024, 1:15 p.m.
    ...Plants Mediated by a Cas9 Gene Containing Multiple Introns.” Plant Communications, vol. 2, no. 2, Elsevier...10.1021/acssynbio.9b00322. Kim, Matthew S., et al. Degron-Based bioPROTACs for Controlling Signaling in CAR...

  24. Prime Editing: Adding Precision and Flexibility to CRISPR Editing

    Type
    Blog Post
    Published
    Jan. 13, 2025, 9:49 p.m.
    ...found that the mutations that emerged depended strongly on the target edit (Doman et al., 2023). Therefore...editors with RT domains derived from the E. coli Ec48 retron RT and the S. pombe Tf1 retrotransposon RT, respectively...

  28. Lucky Thirteen: Guest Contributors of 2023

    Type
    Blog Post
    Published
    Dec. 5, 2023, 2:15 p.m.
    ...Arokiaraj; Viviana Gradinaru; Tim ShayMultifunctional Neuronal Fibers: Modulation from Gut to BrainResearchers...

  29. Expanded Access to Rabies Plasmids

    Type
    Blog Post
    Published
    Feb. 1, 2024, 2:15 p.m.
    ...are used in the lab? Check out our “Rabies and Neuronal Tracing” blog post! You can also browse tools ...

  31. New Podcast Segment: Hot Plasmids

    Type
    Blog Post
    Published
    May 17, 2017, 3:37 p.m.
    ... 2: Magneto 2.0 form Ali Guler's Lab - Control neuronal activity with a magnetic field. Listen to Our ...

  32. New Viral Vectors - Spring 2025

    Type
    Blog Post
    Published
    April 29, 2025, 1:30 p.m.
    ...regulatory element, which targets it to VIP cortical interneurons. Take a look to see if these new preps might...

  33. 2018 #DeckTheLab Contest Winners

    Type
    Blog Post
    Published
    Jan. 15, 2019, 2:19 p.m.
    ...string lights hung up in the shape of RNA, light-up neurons on the lab door, and lot of agar art. This collection...

  36. The Addgene Antibody Data Hub

    Type
    Blog Post
    Published
    Sept. 12, 2024, 1:15 p.m.
    ...antibody performed in an experiment.   Figure 1: The front page of the Addgene Data Hub.   Antibody data reports...

  38. What's New in CRISPR - Winter 2018

    Type
    Blog Post
    Published
    Dec. 18, 2018, 1:17 p.m.
    ...the performance of different nucleases. Nicola Patron’s lab compared the efficiency and specificity of...

  40. Fueled by Coffee at #SfN14

    Type
    Blog Post
    Published
    Nov. 18, 2014, 7:15 p.m.
    ...made in reprogramming astrocytes to functional neurons in vivo. And I’ve seen evidence of the infiltration...
Showing: 361 - 400 of 570 results